$Improved functionality of the vasculature during conventionally fractionated radiation therapy of prostate cancer.$
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Improved functionality of the vasculature during conventionally fractionated radiation therapy of prostate cancer.

Although endothelial cell apoptosis participates in the tumor shrinkage after single high-dose radiotherapy, little is known regarding the vascular response after conventionally fractionated radiation therapy. Therefore, we evaluated hypoxia, perfusion and vascular microenvironment changes in an ort...

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Autores principales:	Vincent A Potiron, Rym Abderrahmani, Karen Clément-Colmou, Séverine Marionneau-Lambot, Thibauld Oullier, François Paris, Stéphane Supiot
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cf58225fd9bc418090e3e5ee62a3bc34
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spelling	oai:doaj.org-article:cf58225fd9bc418090e3e5ee62a3bc342021-11-18T08:39:25ZImproved functionality of the vasculature during conventionally fractionated radiation therapy of prostate cancer.1932-620310.1371/journal.pone.0084076https://doaj.org/article/cf58225fd9bc418090e3e5ee62a3bc342013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24391887/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Although endothelial cell apoptosis participates in the tumor shrinkage after single high-dose radiotherapy, little is known regarding the vascular response after conventionally fractionated radiation therapy. Therefore, we evaluated hypoxia, perfusion and vascular microenvironment changes in an orthotopic prostate cancer model of conventionally fractionated radiation therapy at clinically relevant doses (2 Gy fractions, 5 fractions/week). First, conventionally fractionated radiation therapy decreased tumor cell proliferation and increased cell death with kinetics comparable to human prostate cancer radiotherapy. Secondly, the injection of Hoechst 33342 or fluorescent-dextrans showed an increased tumor perfusion within 14 days in irradiated tumors, which was correlated with a clear reduction of hypoxia. Improved perfusion and decreased hypoxia were not explained by increased blood vessel density, size or network morphology. However, a tumor vascular maturation defined by perivascular desmin+/SMA+ cells coverage was clearly observed along with an increase in endothelial, zonula occludens (ZO)-1 positive, intercellular junctions. Our results show that, in addition to tumor cell killing, vascular maturation plays an uncovered role in tumor reoxygenation during fractionated radiation therapy.Vincent A PotironRym AbderrahmaniKaren Clément-ColmouSéverine Marionneau-LambotThibauld OullierFrançois ParisStéphane SupiotPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e84076 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vincent A Potiron Rym Abderrahmani Karen Clément-Colmou Séverine Marionneau-Lambot Thibauld Oullier François Paris Stéphane Supiot Improved functionality of the vasculature during conventionally fractionated radiation therapy of prostate cancer.
description	Although endothelial cell apoptosis participates in the tumor shrinkage after single high-dose radiotherapy, little is known regarding the vascular response after conventionally fractionated radiation therapy. Therefore, we evaluated hypoxia, perfusion and vascular microenvironment changes in an orthotopic prostate cancer model of conventionally fractionated radiation therapy at clinically relevant doses (2 Gy fractions, 5 fractions/week). First, conventionally fractionated radiation therapy decreased tumor cell proliferation and increased cell death with kinetics comparable to human prostate cancer radiotherapy. Secondly, the injection of Hoechst 33342 or fluorescent-dextrans showed an increased tumor perfusion within 14 days in irradiated tumors, which was correlated with a clear reduction of hypoxia. Improved perfusion and decreased hypoxia were not explained by increased blood vessel density, size or network morphology. However, a tumor vascular maturation defined by perivascular desmin+/SMA+ cells coverage was clearly observed along with an increase in endothelial, zonula occludens (ZO)-1 positive, intercellular junctions. Our results show that, in addition to tumor cell killing, vascular maturation plays an uncovered role in tumor reoxygenation during fractionated radiation therapy.
format	article
author	Vincent A Potiron Rym Abderrahmani Karen Clément-Colmou Séverine Marionneau-Lambot Thibauld Oullier François Paris Stéphane Supiot
author_facet	Vincent A Potiron Rym Abderrahmani Karen Clément-Colmou Séverine Marionneau-Lambot Thibauld Oullier François Paris Stéphane Supiot
author_sort	Vincent A Potiron
title	Improved functionality of the vasculature during conventionally fractionated radiation therapy of prostate cancer.
title_short	Improved functionality of the vasculature during conventionally fractionated radiation therapy of prostate cancer.
title_full	Improved functionality of the vasculature during conventionally fractionated radiation therapy of prostate cancer.
title_fullStr	Improved functionality of the vasculature during conventionally fractionated radiation therapy of prostate cancer.
title_full_unstemmed	Improved functionality of the vasculature during conventionally fractionated radiation therapy of prostate cancer.
title_sort	improved functionality of the vasculature during conventionally fractionated radiation therapy of prostate cancer.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/cf58225fd9bc418090e3e5ee62a3bc34
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Improved functionality of the vasculature during conventionally fractionated radiation therapy of prostate cancer.

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