Evaluating the biological risk of functionalized multiwalled carbon nanotubes and functionalized oxygen-doped multiwalled carbon nanotubes as possible toxic, carcinogenic, and embryotoxic agents

Luis A Lara-Martínez,1 Felipe Massó,2 Eduardo Palacios González,3 Isabel García-Peláez,4 Alejandra Contreras–Ramos,5 Mahara Valverde,6 Emilio Rojas,6 Felipe Cervantes-Sodi,7 Salomón Hernández-Gutiérrez1...

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Autores principales:	Lara-Martínez LA, Massó F, Palacios González E, García-Pelaéz I, Contreras-Ramos A, Valverde M, Rojas E, Cervantes-Sodi F, Hernández-Gutiérrez S
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Carbon Nanotubes Oxigen Doping Functionalization Mesenchymal Stem Cells Cytotoxicity Cellular Transformation Embryotoxicity Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/cf59004ac3d54533adfe8bf684a76ff3
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spelling	oai:doaj.org-article:cf59004ac3d54533adfe8bf684a76ff32021-12-02T02:42:32ZEvaluating the biological risk of functionalized multiwalled carbon nanotubes and functionalized oxygen-doped multiwalled carbon nanotubes as possible toxic, carcinogenic, and embryotoxic agents1178-2013https://doaj.org/article/cf59004ac3d54533adfe8bf684a76ff32017-10-01T00:00:00Zhttps://www.dovepress.com/evaluating-the-biological--risk-of-functionalized-multiwalled-carbon-n-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Luis A Lara-Martínez,1 Felipe Massó,2 Eduardo Palacios González,3 Isabel García-Peláez,4 Alejandra Contreras–Ramos,5 Mahara Valverde,6 Emilio Rojas,6 Felipe Cervantes-Sodi,7 Salomón Hernández-Gutiérrez1 1Department of Molecular Biology, School of Medicine, Universidad Panamericana, Mexico City, Mexico; 2Department of Physiology, National Institute of Cardiology Ignacio Chavez, Mexico City, Mexico; 3Department of Microscopy, Ultra High Resolution Electron Microscopy Laboratory, Instituto Mexicano del Petróleo, Mexico City, Mexico; 4Department of Embryology, Medicine Faculty, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico; 5Department of Developmental Biology Research and Experimental Teratogenicity, Children’s Hospital of Mexico, Federico Gomez, Mexico City, Mexico; 6Department of Genomic Medicine, Institute of Biomedical Research, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico; 7Department of Physics and Mathematics, Nanoscience and Nanotechnology Laboratory, Universidad Iberoamericana, Mexico City, Mexico Abstract: Carbon nanotubes (CNTs) have been a focus of attention due to their possible applications in medicine, by serving as scaffolds for cell growth and proliferation and improving mesenchymal cell transplantation and engraftment. The emphasis on the benefits of CNTs has been offset by the ample debate on the safety of nanotechnologies. In this study, we determine whether functionalized multiwalled CNTs (fMWCNTs) and functionalized oxygen-doped multiwalled CNTs (fCOxs) have toxic effects on rat mesenchymal stem cells (MSCs) in vitro by analyzing morphology and cell proliferation and, using in vivo models, whether they are able to transform MSCs in cancer cells or induce embryotoxicity. Our results demonstrate that there are statistically significant differences in cell proliferation and the cell cycle of MSCs in culture. We identified dramatic changes in cells that were treated with fMWCNTs. Our evaluation of the transformation to cancer cells and cytotoxicity process showed little effect. However, we found a severe embryotoxicity in chicken embryos that were treated with fMWCNTs, while fCOxs seem to exert cardioembryotoxicity and a discrete teratogenicity. Furthermore, it seems that the time of contact plays an important role during cell transformation and embryotoxicity. A single contact with fMWCNTs is not sufficient to transform cells in a short time; an exposure of fMWCNTs for 2 weeks led to cell transformation risk and cardioembryotoxicity effects. Keywords: nanostructure, biocompatibility, scaffold, cell proliferation, cell cycle, carcinogenicLara-Martínez LAMassó FPalacios González EGarcía-Pelaéz IContreras-Ramos AValverde MRojas ECervantes-Sodi FHernández-Gutiérrez SDove Medical PressarticleCarbon NanotubesOxigen DopingFunctionalizationMesenchymal Stem CellsCytotoxicityCellular TransformationEmbryotoxicityMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 7695-7707 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Carbon Nanotubes Oxigen Doping Functionalization Mesenchymal Stem Cells Cytotoxicity Cellular Transformation Embryotoxicity Medicine (General) R5-920
spellingShingle	Carbon Nanotubes Oxigen Doping Functionalization Mesenchymal Stem Cells Cytotoxicity Cellular Transformation Embryotoxicity Medicine (General) R5-920 Lara-Martínez LA Massó F Palacios González E García-Pelaéz I Contreras-Ramos A Valverde M Rojas E Cervantes-Sodi F Hernández-Gutiérrez S Evaluating the biological risk of functionalized multiwalled carbon nanotubes and functionalized oxygen-doped multiwalled carbon nanotubes as possible toxic, carcinogenic, and embryotoxic agents
description	Luis A Lara-Martínez,1 Felipe Massó,2 Eduardo Palacios González,3 Isabel García-Peláez,4 Alejandra Contreras–Ramos,5 Mahara Valverde,6 Emilio Rojas,6 Felipe Cervantes-Sodi,7 Salomón Hernández-Gutiérrez1 1Department of Molecular Biology, School of Medicine, Universidad Panamericana, Mexico City, Mexico; 2Department of Physiology, National Institute of Cardiology Ignacio Chavez, Mexico City, Mexico; 3Department of Microscopy, Ultra High Resolution Electron Microscopy Laboratory, Instituto Mexicano del Petróleo, Mexico City, Mexico; 4Department of Embryology, Medicine Faculty, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico; 5Department of Developmental Biology Research and Experimental Teratogenicity, Children’s Hospital of Mexico, Federico Gomez, Mexico City, Mexico; 6Department of Genomic Medicine, Institute of Biomedical Research, Universidad Nacional Autonoma de Mexico, Mexico City, Mexico; 7Department of Physics and Mathematics, Nanoscience and Nanotechnology Laboratory, Universidad Iberoamericana, Mexico City, Mexico Abstract: Carbon nanotubes (CNTs) have been a focus of attention due to their possible applications in medicine, by serving as scaffolds for cell growth and proliferation and improving mesenchymal cell transplantation and engraftment. The emphasis on the benefits of CNTs has been offset by the ample debate on the safety of nanotechnologies. In this study, we determine whether functionalized multiwalled CNTs (fMWCNTs) and functionalized oxygen-doped multiwalled CNTs (fCOxs) have toxic effects on rat mesenchymal stem cells (MSCs) in vitro by analyzing morphology and cell proliferation and, using in vivo models, whether they are able to transform MSCs in cancer cells or induce embryotoxicity. Our results demonstrate that there are statistically significant differences in cell proliferation and the cell cycle of MSCs in culture. We identified dramatic changes in cells that were treated with fMWCNTs. Our evaluation of the transformation to cancer cells and cytotoxicity process showed little effect. However, we found a severe embryotoxicity in chicken embryos that were treated with fMWCNTs, while fCOxs seem to exert cardioembryotoxicity and a discrete teratogenicity. Furthermore, it seems that the time of contact plays an important role during cell transformation and embryotoxicity. A single contact with fMWCNTs is not sufficient to transform cells in a short time; an exposure of fMWCNTs for 2 weeks led to cell transformation risk and cardioembryotoxicity effects. Keywords: nanostructure, biocompatibility, scaffold, cell proliferation, cell cycle, carcinogenic
format	article
author	Lara-Martínez LA Massó F Palacios González E García-Pelaéz I Contreras-Ramos A Valverde M Rojas E Cervantes-Sodi F Hernández-Gutiérrez S
author_facet	Lara-Martínez LA Massó F Palacios González E García-Pelaéz I Contreras-Ramos A Valverde M Rojas E Cervantes-Sodi F Hernández-Gutiérrez S
author_sort	Lara-Martínez LA
title	Evaluating the biological risk of functionalized multiwalled carbon nanotubes and functionalized oxygen-doped multiwalled carbon nanotubes as possible toxic, carcinogenic, and embryotoxic agents
title_short	Evaluating the biological risk of functionalized multiwalled carbon nanotubes and functionalized oxygen-doped multiwalled carbon nanotubes as possible toxic, carcinogenic, and embryotoxic agents
title_full	Evaluating the biological risk of functionalized multiwalled carbon nanotubes and functionalized oxygen-doped multiwalled carbon nanotubes as possible toxic, carcinogenic, and embryotoxic agents
title_fullStr	Evaluating the biological risk of functionalized multiwalled carbon nanotubes and functionalized oxygen-doped multiwalled carbon nanotubes as possible toxic, carcinogenic, and embryotoxic agents
title_full_unstemmed	Evaluating the biological risk of functionalized multiwalled carbon nanotubes and functionalized oxygen-doped multiwalled carbon nanotubes as possible toxic, carcinogenic, and embryotoxic agents
title_sort	evaluating the biological risk of functionalized multiwalled carbon nanotubes and functionalized oxygen-doped multiwalled carbon nanotubes as possible toxic, carcinogenic, and embryotoxic agents
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/cf59004ac3d54533adfe8bf684a76ff3
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Evaluating the biological risk of functionalized multiwalled carbon nanotubes and functionalized oxygen-doped multiwalled carbon nanotubes as possible toxic, carcinogenic, and embryotoxic agents

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