Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas.

GRF genes have been confirmed to have important regulatory functions in plant growth, development and response to abiotic stress. Although the genome of Jatropha curcas is sequenced, knowledge about the identification of the species' GRF genes and their expression patterns is still lacking. In...

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Autores principales:	Yuehui Tang, Wei Cheng, Shen Li, Ying Li, Xiang Wang, Jiatong Xie, Yingying He, Yaoyu Wang, Yiru Niu, Xinxin Bao, Qian Wu
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cf5cef8da4604b8bb5e04b5f41b0f217
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spelling	oai:doaj.org-article:cf5cef8da4604b8bb5e04b5f41b0f2172021-12-02T20:09:11ZGenome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas.1932-620310.1371/journal.pone.0254711https://doaj.org/article/cf5cef8da4604b8bb5e04b5f41b0f2172021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0254711https://doaj.org/toc/1932-6203GRF genes have been confirmed to have important regulatory functions in plant growth, development and response to abiotic stress. Although the genome of Jatropha curcas is sequenced, knowledge about the identification of the species' GRF genes and their expression patterns is still lacking. In this study, we characterized the 10 JcGRF genes. A detailed investigation into the physic nut GRF gene family is performed, including analysis of the exon-intron structure, conserved domains, conserved motifs, phylogeny, chromosomal locations, potential small RNA targets and expression profiles under both normal growth and abiotic stress conditions. Phylogenetic analysis indicated that the 10 JcGRF genes were classified into five groups corresponding to group I, II, III, IV and V. The analysis of conserved domains showed that the motifs of JcGRF genes were highly conserved in Jatropha curcas. Expression analysis based on RNA-seq and qRT-PCR showed that almost all JcGRF genes had the highest expression in seeds, but very low expression was detected in the non-seed tissues tested, and four JcGRF genes responded to at least one abiotic stress at at least one treatment point. Our research will provide an important scientific basis for further research on the potential functions of JcGRF genes in Jatropha curcas growth and development, and response to abiotic stress, and will eventually provide candidate genes for the breeding of Jatropha curcas.Yuehui TangWei ChengShen LiYing LiXiang WangJiatong XieYingying HeYaoyu WangYiru NiuXinxin BaoQian WuPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 7, p e0254711 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yuehui Tang Wei Cheng Shen Li Ying Li Xiang Wang Jiatong Xie Yingying He Yaoyu Wang Yiru Niu Xinxin Bao Qian Wu Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas.
description	GRF genes have been confirmed to have important regulatory functions in plant growth, development and response to abiotic stress. Although the genome of Jatropha curcas is sequenced, knowledge about the identification of the species' GRF genes and their expression patterns is still lacking. In this study, we characterized the 10 JcGRF genes. A detailed investigation into the physic nut GRF gene family is performed, including analysis of the exon-intron structure, conserved domains, conserved motifs, phylogeny, chromosomal locations, potential small RNA targets and expression profiles under both normal growth and abiotic stress conditions. Phylogenetic analysis indicated that the 10 JcGRF genes were classified into five groups corresponding to group I, II, III, IV and V. The analysis of conserved domains showed that the motifs of JcGRF genes were highly conserved in Jatropha curcas. Expression analysis based on RNA-seq and qRT-PCR showed that almost all JcGRF genes had the highest expression in seeds, but very low expression was detected in the non-seed tissues tested, and four JcGRF genes responded to at least one abiotic stress at at least one treatment point. Our research will provide an important scientific basis for further research on the potential functions of JcGRF genes in Jatropha curcas growth and development, and response to abiotic stress, and will eventually provide candidate genes for the breeding of Jatropha curcas.
format	article
author	Yuehui Tang Wei Cheng Shen Li Ying Li Xiang Wang Jiatong Xie Yingying He Yaoyu Wang Yiru Niu Xinxin Bao Qian Wu
author_facet	Yuehui Tang Wei Cheng Shen Li Ying Li Xiang Wang Jiatong Xie Yingying He Yaoyu Wang Yiru Niu Xinxin Bao Qian Wu
author_sort	Yuehui Tang
title	Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas.
title_short	Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas.
title_full	Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas.
title_fullStr	Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas.
title_full_unstemmed	Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas.
title_sort	genome-wide identification and expression analysis of the growth regulating factor (grf) family in jatropha curcas.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/cf5cef8da4604b8bb5e04b5f41b0f217
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Genome-wide identification and expression analysis of the growth regulating factor (GRF) family in Jatropha curcas.

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