A regulatory network involving Rpo, Gac and Rsm for nitrogen-fixing biofilm formation by Pseudomonas stutzeri
Abstract Biofilm and nitrogen fixation are two competitive strategies used by many plant-associated bacteria; however, the mechanisms underlying the formation of nitrogen-fixing biofilms remain largely unknown. Here, we examined the roles of multiple signalling systems in the regulation of biofilm f...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Nature Portfolio
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/cf5e1c088c564c7c96130fbc8045d8cd |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:cf5e1c088c564c7c96130fbc8045d8cd |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:cf5e1c088c564c7c96130fbc8045d8cd2021-12-02T14:34:03ZA regulatory network involving Rpo, Gac and Rsm for nitrogen-fixing biofilm formation by Pseudomonas stutzeri10.1038/s41522-021-00230-72055-5008https://doaj.org/article/cf5e1c088c564c7c96130fbc8045d8cd2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00230-7https://doaj.org/toc/2055-5008Abstract Biofilm and nitrogen fixation are two competitive strategies used by many plant-associated bacteria; however, the mechanisms underlying the formation of nitrogen-fixing biofilms remain largely unknown. Here, we examined the roles of multiple signalling systems in the regulation of biofilm formation by root-associated diazotrophic P. stutzeri A1501. Physiological analysis, construction of mutant strains and microscale thermophoresis experiments showed that RpoN is a regulatory hub coupling nitrogen fixation and biofilm formation by directly activating the transcription of pslA, a major gene involved in the synthesis of the Psl exopolysaccharide component of the biofilm matrix and nifA, the transcriptional activator of nif gene expression. Genetic complementation studies and determination of the copy number of transcripts by droplet digital PCR confirmed that the regulatory ncRNA RsmZ serves as a signal amplifier to trigger biofilm formation by sequestering the translational repressor protein RsmA away from pslA and sadC mRNAs, the latter of which encodes a diguanylate cyclase that synthesises c-di-GMP. Moreover, RpoS exerts a braking effect on biofilm formation by transcriptionally downregulating RsmZ expression, while RpoS expression is repressed posttranscriptionally by RsmA. These findings provide mechanistic insights into how the Rpo/Gac/Rsm regulatory networks fine-tune nitrogen-fixing biofilm formation in response to the availability of nutrients.Liguo ShangYongliang YanYuhua ZhanXiubin KeYahui ShaoYaqun LiuHua YangShanshan WangShuling DaiJiasi LuNing YanZhimin YangWei LuZhu LiuShanchun ChenClaudine ElmerichMin LinNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-15 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
Microbial ecology QR100-130 |
| spellingShingle |
Microbial ecology QR100-130 Liguo Shang Yongliang Yan Yuhua Zhan Xiubin Ke Yahui Shao Yaqun Liu Hua Yang Shanshan Wang Shuling Dai Jiasi Lu Ning Yan Zhimin Yang Wei Lu Zhu Liu Shanchun Chen Claudine Elmerich Min Lin A regulatory network involving Rpo, Gac and Rsm for nitrogen-fixing biofilm formation by Pseudomonas stutzeri |
| description |
Abstract Biofilm and nitrogen fixation are two competitive strategies used by many plant-associated bacteria; however, the mechanisms underlying the formation of nitrogen-fixing biofilms remain largely unknown. Here, we examined the roles of multiple signalling systems in the regulation of biofilm formation by root-associated diazotrophic P. stutzeri A1501. Physiological analysis, construction of mutant strains and microscale thermophoresis experiments showed that RpoN is a regulatory hub coupling nitrogen fixation and biofilm formation by directly activating the transcription of pslA, a major gene involved in the synthesis of the Psl exopolysaccharide component of the biofilm matrix and nifA, the transcriptional activator of nif gene expression. Genetic complementation studies and determination of the copy number of transcripts by droplet digital PCR confirmed that the regulatory ncRNA RsmZ serves as a signal amplifier to trigger biofilm formation by sequestering the translational repressor protein RsmA away from pslA and sadC mRNAs, the latter of which encodes a diguanylate cyclase that synthesises c-di-GMP. Moreover, RpoS exerts a braking effect on biofilm formation by transcriptionally downregulating RsmZ expression, while RpoS expression is repressed posttranscriptionally by RsmA. These findings provide mechanistic insights into how the Rpo/Gac/Rsm regulatory networks fine-tune nitrogen-fixing biofilm formation in response to the availability of nutrients. |
| format |
article |
| author |
Liguo Shang Yongliang Yan Yuhua Zhan Xiubin Ke Yahui Shao Yaqun Liu Hua Yang Shanshan Wang Shuling Dai Jiasi Lu Ning Yan Zhimin Yang Wei Lu Zhu Liu Shanchun Chen Claudine Elmerich Min Lin |
| author_facet |
Liguo Shang Yongliang Yan Yuhua Zhan Xiubin Ke Yahui Shao Yaqun Liu Hua Yang Shanshan Wang Shuling Dai Jiasi Lu Ning Yan Zhimin Yang Wei Lu Zhu Liu Shanchun Chen Claudine Elmerich Min Lin |
| author_sort |
Liguo Shang |
| title |
A regulatory network involving Rpo, Gac and Rsm for nitrogen-fixing biofilm formation by Pseudomonas stutzeri |
| title_short |
A regulatory network involving Rpo, Gac and Rsm for nitrogen-fixing biofilm formation by Pseudomonas stutzeri |
| title_full |
A regulatory network involving Rpo, Gac and Rsm for nitrogen-fixing biofilm formation by Pseudomonas stutzeri |
| title_fullStr |
A regulatory network involving Rpo, Gac and Rsm for nitrogen-fixing biofilm formation by Pseudomonas stutzeri |
| title_full_unstemmed |
A regulatory network involving Rpo, Gac and Rsm for nitrogen-fixing biofilm formation by Pseudomonas stutzeri |
| title_sort |
regulatory network involving rpo, gac and rsm for nitrogen-fixing biofilm formation by pseudomonas stutzeri |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/cf5e1c088c564c7c96130fbc8045d8cd |
| work_keys_str_mv |
AT liguoshang aregulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT yongliangyan aregulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT yuhuazhan aregulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT xiubinke aregulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT yahuishao aregulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT yaqunliu aregulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT huayang aregulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT shanshanwang aregulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT shulingdai aregulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT jiasilu aregulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT ningyan aregulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT zhiminyang aregulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT weilu aregulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT zhuliu aregulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT shanchunchen aregulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT claudineelmerich aregulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT minlin aregulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT liguoshang regulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT yongliangyan regulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT yuhuazhan regulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT xiubinke regulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT yahuishao regulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT yaqunliu regulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT huayang regulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT shanshanwang regulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT shulingdai regulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT jiasilu regulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT ningyan regulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT zhiminyang regulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT weilu regulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT zhuliu regulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT shanchunchen regulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT claudineelmerich regulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri AT minlin regulatorynetworkinvolvingrpogacandrsmfornitrogenfixingbiofilmformationbypseudomonasstutzeri |
| _version_ |
1718391160136794112 |