Long non-coding RNA (lncRNA) five prime to Xist (FTX) promotes retinoblastoma progression by regulating the microRNA-320a/with-nolysine kinases 1 (WNK1) axis: Running Head: LncRNA FTX promotes retinoblastoma

Long non-coding RNA (lncRNA) five prime to Xist (FTX) exerts important functions in human cancer, while its role in retinoblastoma (RB) remains unclear. This study aimed to investigate the role of FTX in RB. The expression levels of FTX were assessed by quantitative real-time polymerase chain reacti...

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Autores principales:	Xiaolei Wang, Yu Su, Chuangao Yin
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	retinoblastoma ftx mir-320a wnk1 Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/cf663bf1365646128fe1d44c9f7d498e
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spelling	oai:doaj.org-article:cf663bf1365646128fe1d44c9f7d498e2021-11-04T15:51:54ZLong non-coding RNA (lncRNA) five prime to Xist (FTX) promotes retinoblastoma progression by regulating the microRNA-320a/with-nolysine kinases 1 (WNK1) axis: Running Head: LncRNA FTX promotes retinoblastoma2165-59792165-598710.1080/21655979.2021.1994718https://doaj.org/article/cf663bf1365646128fe1d44c9f7d498e2021-11-01T00:00:00Zhttp://dx.doi.org/10.1080/21655979.2021.1994718https://doaj.org/toc/2165-5979https://doaj.org/toc/2165-5987Long non-coding RNA (lncRNA) five prime to Xist (FTX) exerts important functions in human cancer, while its role in retinoblastoma (RB) remains unclear. This study aimed to investigate the role of FTX in RB. The expression levels of FTX were assessed by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation was evaluated by cell counting kit-8 (CCK-8), 5‐ethynyl‐2′‐deoxyuridine (EdU) staining and colony formation assays. Cell migration and invasion were detected by Transwell assay. The relationship among FTX, microRNA-320a (miR-320a) and with-no lysine kinase 1 (WNK1) was also investigated. In the present study, we found that the expression levels of FTX were notably elevated in RB tissues and cancer cell lines. Overexpression of FTX exacerbated the aggressive phenotypes (cell proliferation, migration and invasion) of RB cells. Downregulation of miR-320a obviously attenuated the inhibitory effects of knockdown of FTX in RB malignant phenotypes, and knockdown of WNK1 also reversed the impacts of miR-320a inhibitor on malignant phenotypes. In vivo experiments further confirmed that knockdown of FTX efficiently prevent tumor growth in vivo. Our results revealed that FTX promoted RB progression by targeting the miR-320a/WNK1 axis (graphical abstract), suggesting that FTX might be a novel therapeutic target for RB.Xiaolei WangYu SuChuangao YinTaylor & Francis Grouparticleretinoblastomaftxmir-320awnk1BiotechnologyTP248.13-248.65ENBioengineered, Vol 0, Iss 0 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	retinoblastoma ftx mir-320a wnk1 Biotechnology TP248.13-248.65
spellingShingle	retinoblastoma ftx mir-320a wnk1 Biotechnology TP248.13-248.65 Xiaolei Wang Yu Su Chuangao Yin Long non-coding RNA (lncRNA) five prime to Xist (FTX) promotes retinoblastoma progression by regulating the microRNA-320a/with-nolysine kinases 1 (WNK1) axis: Running Head: LncRNA FTX promotes retinoblastoma
description	Long non-coding RNA (lncRNA) five prime to Xist (FTX) exerts important functions in human cancer, while its role in retinoblastoma (RB) remains unclear. This study aimed to investigate the role of FTX in RB. The expression levels of FTX were assessed by quantitative real-time polymerase chain reaction (qRT-PCR). Cell proliferation was evaluated by cell counting kit-8 (CCK-8), 5‐ethynyl‐2′‐deoxyuridine (EdU) staining and colony formation assays. Cell migration and invasion were detected by Transwell assay. The relationship among FTX, microRNA-320a (miR-320a) and with-no lysine kinase 1 (WNK1) was also investigated. In the present study, we found that the expression levels of FTX were notably elevated in RB tissues and cancer cell lines. Overexpression of FTX exacerbated the aggressive phenotypes (cell proliferation, migration and invasion) of RB cells. Downregulation of miR-320a obviously attenuated the inhibitory effects of knockdown of FTX in RB malignant phenotypes, and knockdown of WNK1 also reversed the impacts of miR-320a inhibitor on malignant phenotypes. In vivo experiments further confirmed that knockdown of FTX efficiently prevent tumor growth in vivo. Our results revealed that FTX promoted RB progression by targeting the miR-320a/WNK1 axis (graphical abstract), suggesting that FTX might be a novel therapeutic target for RB.
format	article
author	Xiaolei Wang Yu Su Chuangao Yin
author_facet	Xiaolei Wang Yu Su Chuangao Yin
author_sort	Xiaolei Wang
title	Long non-coding RNA (lncRNA) five prime to Xist (FTX) promotes retinoblastoma progression by regulating the microRNA-320a/with-nolysine kinases 1 (WNK1) axis: Running Head: LncRNA FTX promotes retinoblastoma
title_short	Long non-coding RNA (lncRNA) five prime to Xist (FTX) promotes retinoblastoma progression by regulating the microRNA-320a/with-nolysine kinases 1 (WNK1) axis: Running Head: LncRNA FTX promotes retinoblastoma
title_full	Long non-coding RNA (lncRNA) five prime to Xist (FTX) promotes retinoblastoma progression by regulating the microRNA-320a/with-nolysine kinases 1 (WNK1) axis: Running Head: LncRNA FTX promotes retinoblastoma
title_fullStr	Long non-coding RNA (lncRNA) five prime to Xist (FTX) promotes retinoblastoma progression by regulating the microRNA-320a/with-nolysine kinases 1 (WNK1) axis: Running Head: LncRNA FTX promotes retinoblastoma
title_full_unstemmed	Long non-coding RNA (lncRNA) five prime to Xist (FTX) promotes retinoblastoma progression by regulating the microRNA-320a/with-nolysine kinases 1 (WNK1) axis: Running Head: LncRNA FTX promotes retinoblastoma
title_sort	long non-coding rna (lncrna) five prime to xist (ftx) promotes retinoblastoma progression by regulating the microrna-320a/with-nolysine kinases 1 (wnk1) axis: running head: lncrna ftx promotes retinoblastoma
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/cf663bf1365646128fe1d44c9f7d498e
work_keys_str_mv	AT xiaoleiwang longnoncodingrnalncrnafiveprimetoxistftxpromotesretinoblastomaprogressionbyregulatingthemicrorna320awithnolysinekinases1wnk1axisrunningheadlncrnaftxpromotesretinoblastoma AT yusu longnoncodingrnalncrnafiveprimetoxistftxpromotesretinoblastomaprogressionbyregulatingthemicrorna320awithnolysinekinases1wnk1axisrunningheadlncrnaftxpromotesretinoblastoma AT chuangaoyin longnoncodingrnalncrnafiveprimetoxistftxpromotesretinoblastomaprogressionbyregulatingthemicrorna320awithnolysinekinases1wnk1axisrunningheadlncrnaftxpromotesretinoblastoma
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Long non-coding RNA (lncRNA) five prime to Xist (FTX) promotes retinoblastoma progression by regulating the microRNA-320a/with-nolysine kinases 1 (WNK1) axis: Running Head: LncRNA FTX promotes retinoblastoma

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