Sequence-Related Amplified Polymorphism (SRAP) analysis for studying genetic characterization of Bouea macrophylla

Kaewpongumpai S, Poeaim S, Vanijajiva O. 2016. Sequence-Related Amplified Polymorphism (SRAP) analysis for studying genetic characterization of Bouea macrophylla. Biodiversitas 17: 539-543. Bouea macrophylla Griff. is well-known as one of native typical fruits in Southeast Asia which needs to be pre...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	SOMBHAT KAEWPONGUMPAI, SUPATTRA POEAIM, ONGKARN VANIJAJIVA
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2016
Materias:	bouea macrophylla, srap, genetic characterization Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/cf6a44f54a1f4b79b341e0332244a8e7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cf6a44f54a1f4b79b341e0332244a8e7
record_format	dspace
spelling	oai:doaj.org-article:cf6a44f54a1f4b79b341e0332244a8e72021-11-14T02:27:09ZSequence-Related Amplified Polymorphism (SRAP) analysis for studying genetic characterization of Bouea macrophylla1412-033X2085-472210.13057/biodiv/d170224https://doaj.org/article/cf6a44f54a1f4b79b341e0332244a8e72016-06-01T00:00:00Zhttps://smujo.id/biodiv/article/view/328https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Kaewpongumpai S, Poeaim S, Vanijajiva O. 2016. Sequence-Related Amplified Polymorphism (SRAP) analysis for studying genetic characterization of Bouea macrophylla. Biodiversitas 17: 539-543. Bouea macrophylla Griff. is well-known as one of native typical fruits in Southeast Asia which needs to be preserved and continuously cultivated because of economical and ecological significances. More recently, sequence-related amplified polymorphism (SRAP) markers have been developed, which are used to amplify coding regions of DNA with primers targeting open reading frames. This technique has proven to be robust and highly variable and is attained through a significantly less technically demanding process. In this research, SRAP method was preliminary applied to assess genetic characterization of B. macrophylla. Genomic DNA was extracted from fresh leaf samples. The result clearly showed that at 100 ng template DNA and MgCl2 5 mM concentration are suitable for further PCR analysis. Thirty SRAP primer combinations were initially screened for analysis and 26 primer combinations were chosen for further analysis. A total of 222 DNA fragments, varying from 90-2500 bp, were amplified. The produced band number for each optimal primer set ranged from 3 to 12 with a percentage of polymorphic bands spanning from 33.33 to 80.00%. Therefore, SRAP analysis is suitable for further analysis method on genetic study of Bouea species and related genera.SOMBHAT KAEWPONGUMPAISUPATTRA POEAIMONGKARN VANIJAJIVAMBI & UNS Soloarticlebouea macrophylla, srap, genetic characterizationBiology (General)QH301-705.5ENBiodiversitas, Vol 17, Iss 2 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	bouea macrophylla, srap, genetic characterization Biology (General) QH301-705.5
spellingShingle	bouea macrophylla, srap, genetic characterization Biology (General) QH301-705.5 SOMBHAT KAEWPONGUMPAI SUPATTRA POEAIM ONGKARN VANIJAJIVA Sequence-Related Amplified Polymorphism (SRAP) analysis for studying genetic characterization of Bouea macrophylla
description	Kaewpongumpai S, Poeaim S, Vanijajiva O. 2016. Sequence-Related Amplified Polymorphism (SRAP) analysis for studying genetic characterization of Bouea macrophylla. Biodiversitas 17: 539-543. Bouea macrophylla Griff. is well-known as one of native typical fruits in Southeast Asia which needs to be preserved and continuously cultivated because of economical and ecological significances. More recently, sequence-related amplified polymorphism (SRAP) markers have been developed, which are used to amplify coding regions of DNA with primers targeting open reading frames. This technique has proven to be robust and highly variable and is attained through a significantly less technically demanding process. In this research, SRAP method was preliminary applied to assess genetic characterization of B. macrophylla. Genomic DNA was extracted from fresh leaf samples. The result clearly showed that at 100 ng template DNA and MgCl2 5 mM concentration are suitable for further PCR analysis. Thirty SRAP primer combinations were initially screened for analysis and 26 primer combinations were chosen for further analysis. A total of 222 DNA fragments, varying from 90-2500 bp, were amplified. The produced band number for each optimal primer set ranged from 3 to 12 with a percentage of polymorphic bands spanning from 33.33 to 80.00%. Therefore, SRAP analysis is suitable for further analysis method on genetic study of Bouea species and related genera.
format	article
author	SOMBHAT KAEWPONGUMPAI SUPATTRA POEAIM ONGKARN VANIJAJIVA
author_facet	SOMBHAT KAEWPONGUMPAI SUPATTRA POEAIM ONGKARN VANIJAJIVA
author_sort	SOMBHAT KAEWPONGUMPAI
title	Sequence-Related Amplified Polymorphism (SRAP) analysis for studying genetic characterization of Bouea macrophylla
title_short	Sequence-Related Amplified Polymorphism (SRAP) analysis for studying genetic characterization of Bouea macrophylla
title_full	Sequence-Related Amplified Polymorphism (SRAP) analysis for studying genetic characterization of Bouea macrophylla
title_fullStr	Sequence-Related Amplified Polymorphism (SRAP) analysis for studying genetic characterization of Bouea macrophylla
title_full_unstemmed	Sequence-Related Amplified Polymorphism (SRAP) analysis for studying genetic characterization of Bouea macrophylla
title_sort	sequence-related amplified polymorphism (srap) analysis for studying genetic characterization of bouea macrophylla
publisher	MBI & UNS Solo
publishDate	2016
url	https://doaj.org/article/cf6a44f54a1f4b79b341e0332244a8e7
work_keys_str_mv	AT sombhatkaewpongumpai sequencerelatedamplifiedpolymorphismsrapanalysisforstudyinggeneticcharacterizationofboueamacrophylla AT supattrapoeaim sequencerelatedamplifiedpolymorphismsrapanalysisforstudyinggeneticcharacterizationofboueamacrophylla AT ongkarnvanijajiva sequencerelatedamplifiedpolymorphismsrapanalysisforstudyinggeneticcharacterizationofboueamacrophylla
_version_	1718430043738210304

Sequence-Related Amplified Polymorphism (SRAP) analysis for studying genetic characterization of Bouea macrophylla

Ejemplares similares