Identification and phylogenetic analysis of two canine coronavirus strains

Abstract Canine coronavirus (CCoV), a member of the genus Alphacoronavirus, is an enveloped, single-stranded positive-sense RNA virus that responsible for gastroenteritis in dogs. In this study, two CCoV isolates were successfully propagated from 53 CCoV-positive clinical specimens by serial passagi...

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Autores principales:	Junji Gan, Ye Tang, Haifeng Lv, Wenbin Xiong, Xiaoyan Tian
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Canine coronavirus Characterization IIa subtype Isolation Phylogenetic analysis Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270
Acceso en línea:	https://doaj.org/article/cf77e2c6f3964f51976abbfce93aed15
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spelling	oai:doaj.org-article:cf77e2c6f3964f51976abbfce93aed152021-11-14T12:06:07ZIdentification and phylogenetic analysis of two canine coronavirus strains10.1186/s44149-021-00013-92731-0442https://doaj.org/article/cf77e2c6f3964f51976abbfce93aed152021-07-01T00:00:00Zhttps://doi.org/10.1186/s44149-021-00013-9https://doaj.org/toc/2731-0442Abstract Canine coronavirus (CCoV), a member of the genus Alphacoronavirus, is an enveloped, single-stranded positive-sense RNA virus that responsible for gastroenteritis in dogs. In this study, two CCoV isolates were successfully propagated from 53 CCoV-positive clinical specimens by serial passaging in A-72 cells. These two strains, CCoV JS1706 and CCoV JS1712, caused cytopathic effects in A-72 cells. The sizes of virus plaque formed by them differed in early passages. Electron microscopy revealed a large quantity of typical coronavirus particles with 80–120 nm in diameter in cell culture media and cytoplasm of infected cells, in which they appeared as inclusion bodies. RT-PCR analysis of S gene indicated that these two isolates were belonged to CCoV IIa subtype. Homology of RdRp, S, M and N proteins between the two strains were 100, 99.6, 99.2 and 100.0%, respectively, whereas they were 99.4–100%, 83.1–95.2%, 88.5–99.2% and 91.9–99.7% identity compared to CCoV II reference strains. Phylogenetic analysis of RdRp, S, M and N protein showed that they were closely related to CCoV II strains. These two subtype IIa isolates will be useful for evaluating the pathogenesis and evolution of CCoV and for developing diagnostic reagents and vaccines.Junji GanYe TangHaifeng LvWenbin XiongXiaoyan TianBMCarticleCanine coronavirusCharacterizationIIa subtypeIsolationPhylogenetic analysisVeterinary medicineSF600-1100Public aspects of medicineRA1-1270ENAnimal Diseases, Vol 1, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Canine coronavirus Characterization IIa subtype Isolation Phylogenetic analysis Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270
spellingShingle	Canine coronavirus Characterization IIa subtype Isolation Phylogenetic analysis Veterinary medicine SF600-1100 Public aspects of medicine RA1-1270 Junji Gan Ye Tang Haifeng Lv Wenbin Xiong Xiaoyan Tian Identification and phylogenetic analysis of two canine coronavirus strains
description	Abstract Canine coronavirus (CCoV), a member of the genus Alphacoronavirus, is an enveloped, single-stranded positive-sense RNA virus that responsible for gastroenteritis in dogs. In this study, two CCoV isolates were successfully propagated from 53 CCoV-positive clinical specimens by serial passaging in A-72 cells. These two strains, CCoV JS1706 and CCoV JS1712, caused cytopathic effects in A-72 cells. The sizes of virus plaque formed by them differed in early passages. Electron microscopy revealed a large quantity of typical coronavirus particles with 80–120 nm in diameter in cell culture media and cytoplasm of infected cells, in which they appeared as inclusion bodies. RT-PCR analysis of S gene indicated that these two isolates were belonged to CCoV IIa subtype. Homology of RdRp, S, M and N proteins between the two strains were 100, 99.6, 99.2 and 100.0%, respectively, whereas they were 99.4–100%, 83.1–95.2%, 88.5–99.2% and 91.9–99.7% identity compared to CCoV II reference strains. Phylogenetic analysis of RdRp, S, M and N protein showed that they were closely related to CCoV II strains. These two subtype IIa isolates will be useful for evaluating the pathogenesis and evolution of CCoV and for developing diagnostic reagents and vaccines.
format	article
author	Junji Gan Ye Tang Haifeng Lv Wenbin Xiong Xiaoyan Tian
author_facet	Junji Gan Ye Tang Haifeng Lv Wenbin Xiong Xiaoyan Tian
author_sort	Junji Gan
title	Identification and phylogenetic analysis of two canine coronavirus strains
title_short	Identification and phylogenetic analysis of two canine coronavirus strains
title_full	Identification and phylogenetic analysis of two canine coronavirus strains
title_fullStr	Identification and phylogenetic analysis of two canine coronavirus strains
title_full_unstemmed	Identification and phylogenetic analysis of two canine coronavirus strains
title_sort	identification and phylogenetic analysis of two canine coronavirus strains
publisher	BMC
publishDate	2021
url	https://doaj.org/article/cf77e2c6f3964f51976abbfce93aed15
work_keys_str_mv	AT junjigan identificationandphylogeneticanalysisoftwocaninecoronavirusstrains AT yetang identificationandphylogeneticanalysisoftwocaninecoronavirusstrains AT haifenglv identificationandphylogeneticanalysisoftwocaninecoronavirusstrains AT wenbinxiong identificationandphylogeneticanalysisoftwocaninecoronavirusstrains AT xiaoyantian identificationandphylogeneticanalysisoftwocaninecoronavirusstrains
_version_	1718429492201914368

Identification and phylogenetic analysis of two canine coronavirus strains

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