In Vitro and In Vivo Toxicity Evaluation of Natural Products with Potential Applications as Biopesticides

The use of natural products in agriculture as pesticides has been strongly advocated. However, it is necessary to assess their toxicity to ensure their safe use. In the present study, mammalian cell lines and fish models of the zebrafish (<i>Danio rerio)</i> and medaka (<i>Oryzias...

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Autores principales:	Felicia Sangermano, Marco Masi, Amrish Kumar, Ravindra Peravali, Angela Tuzi, Alessio Cimmino, Daniela Vallone, Giuliana Giamundo, Ivan Conte, Antonio Evidente, Viola Calabrò
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	plant and microbial metabolites cyclopaldic and α-costic acids agricultural application cytotoxicity DNA damage developmental toxicity Medicine R
Acceso en línea:	https://doaj.org/article/cf80545af7f34ffb9133ccd7dd50c254
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spelling	oai:doaj.org-article:cf80545af7f34ffb9133ccd7dd50c2542021-11-25T19:08:58ZIn Vitro and In Vivo Toxicity Evaluation of Natural Products with Potential Applications as Biopesticides10.3390/toxins131108052072-6651https://doaj.org/article/cf80545af7f34ffb9133ccd7dd50c2542021-11-01T00:00:00Zhttps://www.mdpi.com/2072-6651/13/11/805https://doaj.org/toc/2072-6651The use of natural products in agriculture as pesticides has been strongly advocated. However, it is necessary to assess their toxicity to ensure their safe use. In the present study, mammalian cell lines and fish models of the zebrafish (<i>Danio rerio)</i> and medaka (<i>Oryzias latipes</i>) have been used to investigate the toxic effects of ten natural products which have potential applications as biopesticides. The fungal metabolites cavoxin, <i>epi</i>-epoformin, papyracillic acid, seiridin and sphaeropsidone, together with the plant compounds inuloxins A and C and ungeremine, showed no toxic effects in mammalian cells and zebrafish embryos. Conversely, cyclopaldic and α-costic acids, produced by <i>Seiridium cupressi</i> and <i>Dittrichia viscosa,</i> respectively, caused significant mortality in zebrafish and medaka embryos as a result of yolk coagulation. However, both compounds showed little effect in zebrafish or mammalian cell lines in culture, thus highlighting the importance of the fish embryotoxicity test in the assessment of environmental impact. Given the embryotoxicity of α-costic acid and cyclopaldic acid, their use as biopesticides is not recommended. Further ecotoxicological studies are needed to evaluate the potential applications of the other compounds.Felicia SangermanoMarco MasiAmrish KumarRavindra PeravaliAngela TuziAlessio CimminoDaniela ValloneGiuliana GiamundoIvan ConteAntonio EvidenteViola CalabròMDPI AGarticleplant and microbial metabolitescyclopaldic and α-costic acidsagricultural applicationcytotoxicityDNA damagedevelopmental toxicityMedicineRENToxins, Vol 13, Iss 805, p 805 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	plant and microbial metabolites cyclopaldic and α-costic acids agricultural application cytotoxicity DNA damage developmental toxicity Medicine R
spellingShingle	plant and microbial metabolites cyclopaldic and α-costic acids agricultural application cytotoxicity DNA damage developmental toxicity Medicine R Felicia Sangermano Marco Masi Amrish Kumar Ravindra Peravali Angela Tuzi Alessio Cimmino Daniela Vallone Giuliana Giamundo Ivan Conte Antonio Evidente Viola Calabrò In Vitro and In Vivo Toxicity Evaluation of Natural Products with Potential Applications as Biopesticides
description	The use of natural products in agriculture as pesticides has been strongly advocated. However, it is necessary to assess their toxicity to ensure their safe use. In the present study, mammalian cell lines and fish models of the zebrafish (<i>Danio rerio)</i> and medaka (<i>Oryzias latipes</i>) have been used to investigate the toxic effects of ten natural products which have potential applications as biopesticides. The fungal metabolites cavoxin, <i>epi</i>-epoformin, papyracillic acid, seiridin and sphaeropsidone, together with the plant compounds inuloxins A and C and ungeremine, showed no toxic effects in mammalian cells and zebrafish embryos. Conversely, cyclopaldic and α-costic acids, produced by <i>Seiridium cupressi</i> and <i>Dittrichia viscosa,</i> respectively, caused significant mortality in zebrafish and medaka embryos as a result of yolk coagulation. However, both compounds showed little effect in zebrafish or mammalian cell lines in culture, thus highlighting the importance of the fish embryotoxicity test in the assessment of environmental impact. Given the embryotoxicity of α-costic acid and cyclopaldic acid, their use as biopesticides is not recommended. Further ecotoxicological studies are needed to evaluate the potential applications of the other compounds.
format	article
author	Felicia Sangermano Marco Masi Amrish Kumar Ravindra Peravali Angela Tuzi Alessio Cimmino Daniela Vallone Giuliana Giamundo Ivan Conte Antonio Evidente Viola Calabrò
author_facet	Felicia Sangermano Marco Masi Amrish Kumar Ravindra Peravali Angela Tuzi Alessio Cimmino Daniela Vallone Giuliana Giamundo Ivan Conte Antonio Evidente Viola Calabrò
author_sort	Felicia Sangermano
title	In Vitro and In Vivo Toxicity Evaluation of Natural Products with Potential Applications as Biopesticides
title_short	In Vitro and In Vivo Toxicity Evaluation of Natural Products with Potential Applications as Biopesticides
title_full	In Vitro and In Vivo Toxicity Evaluation of Natural Products with Potential Applications as Biopesticides
title_fullStr	In Vitro and In Vivo Toxicity Evaluation of Natural Products with Potential Applications as Biopesticides
title_full_unstemmed	In Vitro and In Vivo Toxicity Evaluation of Natural Products with Potential Applications as Biopesticides
title_sort	in vitro and in vivo toxicity evaluation of natural products with potential applications as biopesticides
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cf80545af7f34ffb9133ccd7dd50c254
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In Vitro and In Vivo Toxicity Evaluation of Natural Products with Potential Applications as Biopesticides

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