Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering

The heterochromatic knob (hk4S) on Arabidopsis chromosome 4 prevents the recombination between accessions with and without hk4S. Here, via egg-cell specific expression of the Cas9 nuclease, the authors demonstrate targeted reversal of the 1.1 Mb long hk4S-inversion in Col-0 and restore the crossover...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Carla Schmidt, Paul Fransz, Michelle Rönspies, Steven Dreissig, Jörg Fuchs, Stefan Heckmann, Andreas Houben, Holger Puchta
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/cf83736280954c2b9ce44781961ab796
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cf83736280954c2b9ce44781961ab796
record_format	dspace
spelling	oai:doaj.org-article:cf83736280954c2b9ce44781961ab7962021-12-02T15:27:35ZChanging local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering10.1038/s41467-020-18277-z2041-1723https://doaj.org/article/cf83736280954c2b9ce44781961ab7962020-09-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-18277-zhttps://doaj.org/toc/2041-1723The heterochromatic knob (hk4S) on Arabidopsis chromosome 4 prevents the recombination between accessions with and without hk4S. Here, via egg-cell specific expression of the Cas9 nuclease, the authors demonstrate targeted reversal of the 1.1 Mb long hk4S-inversion in Col-0 and restore the crossovers with Ler-1.Carla SchmidtPaul FranszMichelle RönspiesSteven DreissigJörg FuchsStefan HeckmannAndreas HoubenHolger PuchtaNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-8 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Carla Schmidt Paul Fransz Michelle Rönspies Steven Dreissig Jörg Fuchs Stefan Heckmann Andreas Houben Holger Puchta Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering
description	The heterochromatic knob (hk4S) on Arabidopsis chromosome 4 prevents the recombination between accessions with and without hk4S. Here, via egg-cell specific expression of the Cas9 nuclease, the authors demonstrate targeted reversal of the 1.1 Mb long hk4S-inversion in Col-0 and restore the crossovers with Ler-1.
format	article
author	Carla Schmidt Paul Fransz Michelle Rönspies Steven Dreissig Jörg Fuchs Stefan Heckmann Andreas Houben Holger Puchta
author_facet	Carla Schmidt Paul Fransz Michelle Rönspies Steven Dreissig Jörg Fuchs Stefan Heckmann Andreas Houben Holger Puchta
author_sort	Carla Schmidt
title	Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering
title_short	Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering
title_full	Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering
title_fullStr	Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering
title_full_unstemmed	Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering
title_sort	changing local recombination patterns in arabidopsis by crispr/cas mediated chromosome engineering
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/cf83736280954c2b9ce44781961ab796
work_keys_str_mv	AT carlaschmidt changinglocalrecombinationpatternsinarabidopsisbycrisprcasmediatedchromosomeengineering AT paulfransz changinglocalrecombinationpatternsinarabidopsisbycrisprcasmediatedchromosomeengineering AT michelleronspies changinglocalrecombinationpatternsinarabidopsisbycrisprcasmediatedchromosomeengineering AT stevendreissig changinglocalrecombinationpatternsinarabidopsisbycrisprcasmediatedchromosomeengineering AT jorgfuchs changinglocalrecombinationpatternsinarabidopsisbycrisprcasmediatedchromosomeengineering AT stefanheckmann changinglocalrecombinationpatternsinarabidopsisbycrisprcasmediatedchromosomeengineering AT andreashouben changinglocalrecombinationpatternsinarabidopsisbycrisprcasmediatedchromosomeengineering AT holgerpuchta changinglocalrecombinationpatternsinarabidopsisbycrisprcasmediatedchromosomeengineering
_version_	1718387230656954368

Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering

Ejemplares similares