Changing local recombination patterns in Arabidopsis by CRISPR/Cas mediated chromosome engineering

The heterochromatic knob (hk4S) on Arabidopsis chromosome 4 prevents the recombination between accessions with and without hk4S. Here, via egg-cell specific expression of the Cas9 nuclease, the authors demonstrate targeted reversal of the 1.1 Mb long hk4S-inversion in Col-0 and restore the crossover...

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Autores principales: Carla Schmidt, Paul Fransz, Michelle Rönspies, Steven Dreissig, Jörg Fuchs, Stefan Heckmann, Andreas Houben, Holger Puchta
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2020
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Acceso en línea:https://doaj.org/article/cf83736280954c2b9ce44781961ab796
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