An Optimized Method for Extraction and Characterization of Phenolic Compounds in Dendranthema indicum var. aromaticum Flower

Abstract Dendranthema indicum var. aromaticum plant has been widely used as herbal medicine in China, however, the material basis responsible for the therapeutic benefits remains largely unclear. This study aimed to provide an optimized method for extracting and characterizing phenolic compounds in...

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Autores principales: Lijie Zhong, Zhiyang Yuan, Lin Rong, Yaohua Zhang, Guoxi Xiong, Yi Liu, Chao Li
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Publicado: Nature Portfolio 2019
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spelling oai:doaj.org-article:cf86ecaf06544bed8c8961add886ef1e2021-12-02T15:08:07ZAn Optimized Method for Extraction and Characterization of Phenolic Compounds in Dendranthema indicum var. aromaticum Flower10.1038/s41598-019-44102-92045-2322https://doaj.org/article/cf86ecaf06544bed8c8961add886ef1e2019-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-44102-9https://doaj.org/toc/2045-2322Abstract Dendranthema indicum var. aromaticum plant has been widely used as herbal medicine in China, however, the material basis responsible for the therapeutic benefits remains largely unclear. This study aimed to provide an optimized method for extracting and characterizing phenolic compounds in D. indicum var. aromaticum flower. Firstly, an ultrasound-assisted method combined with central composite circumscribed (CCC) design was applied to optimize phenolic compound extraction. Ethanol-acetic acid (70%:2%, v/v) was selected as solvent, and the optimal extraction condition was: extraction temperature, 57 °C; solid/liquid ratio, 1:30 g/mL; extraction time, 20 min. Secondly, an effective and economic HPLC-PDA-ESI-MSn method was established and validated for phenolic compound characterization and quantification. As a result, 14 phenolic compounds were identified, including 8 phenolic acids and 6 flavonoids, and for the first time, oleuropein derivatives, chrysoeriol, and tricin are reported in D. indicum var. aromaticum flower. The content of phenolics identified by HPLC-MSn was 6.42 ± 0.32 mg/g DW. The optimized method for extraction and characterization of phenolic compounds has significant meaning to future pharmaceutical and medicinal research on D. indicum var. aromaticum, and the results in this study can provide references for herbal research.Lijie ZhongZhiyang YuanLin RongYaohua ZhangGuoxi XiongYi LiuChao LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-12 (2019)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Lijie Zhong
Zhiyang Yuan
Lin Rong
Yaohua Zhang
Guoxi Xiong
Yi Liu
Chao Li
An Optimized Method for Extraction and Characterization of Phenolic Compounds in Dendranthema indicum var. aromaticum Flower
description Abstract Dendranthema indicum var. aromaticum plant has been widely used as herbal medicine in China, however, the material basis responsible for the therapeutic benefits remains largely unclear. This study aimed to provide an optimized method for extracting and characterizing phenolic compounds in D. indicum var. aromaticum flower. Firstly, an ultrasound-assisted method combined with central composite circumscribed (CCC) design was applied to optimize phenolic compound extraction. Ethanol-acetic acid (70%:2%, v/v) was selected as solvent, and the optimal extraction condition was: extraction temperature, 57 °C; solid/liquid ratio, 1:30 g/mL; extraction time, 20 min. Secondly, an effective and economic HPLC-PDA-ESI-MSn method was established and validated for phenolic compound characterization and quantification. As a result, 14 phenolic compounds were identified, including 8 phenolic acids and 6 flavonoids, and for the first time, oleuropein derivatives, chrysoeriol, and tricin are reported in D. indicum var. aromaticum flower. The content of phenolics identified by HPLC-MSn was 6.42 ± 0.32 mg/g DW. The optimized method for extraction and characterization of phenolic compounds has significant meaning to future pharmaceutical and medicinal research on D. indicum var. aromaticum, and the results in this study can provide references for herbal research.
format article
author Lijie Zhong
Zhiyang Yuan
Lin Rong
Yaohua Zhang
Guoxi Xiong
Yi Liu
Chao Li
author_facet Lijie Zhong
Zhiyang Yuan
Lin Rong
Yaohua Zhang
Guoxi Xiong
Yi Liu
Chao Li
author_sort Lijie Zhong
title An Optimized Method for Extraction and Characterization of Phenolic Compounds in Dendranthema indicum var. aromaticum Flower
title_short An Optimized Method for Extraction and Characterization of Phenolic Compounds in Dendranthema indicum var. aromaticum Flower
title_full An Optimized Method for Extraction and Characterization of Phenolic Compounds in Dendranthema indicum var. aromaticum Flower
title_fullStr An Optimized Method for Extraction and Characterization of Phenolic Compounds in Dendranthema indicum var. aromaticum Flower
title_full_unstemmed An Optimized Method for Extraction and Characterization of Phenolic Compounds in Dendranthema indicum var. aromaticum Flower
title_sort optimized method for extraction and characterization of phenolic compounds in dendranthema indicum var. aromaticum flower
publisher Nature Portfolio
publishDate 2019
url https://doaj.org/article/cf86ecaf06544bed8c8961add886ef1e
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