Automated Segmentation of Light-Sheet Fluorescent Imaging to Characterize Experimental Doxorubicin-Induced Cardiac Injury and Repair
Abstract This study sought to develop an automated segmentation approach based on histogram analysis of raw axial images acquired by light-sheet fluorescent imaging (LSFI) to establish rapid reconstruction of the 3-D zebrafish cardiac architecture in response to doxorubicin-induced injury and repair...
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Nature Portfolio
2017
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oai:doaj.org-article:cf8a7b60f3f1442096de0430ac596c002021-12-02T11:40:59ZAutomated Segmentation of Light-Sheet Fluorescent Imaging to Characterize Experimental Doxorubicin-Induced Cardiac Injury and Repair10.1038/s41598-017-09152-x2045-2322https://doaj.org/article/cf8a7b60f3f1442096de0430ac596c002017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09152-xhttps://doaj.org/toc/2045-2322Abstract This study sought to develop an automated segmentation approach based on histogram analysis of raw axial images acquired by light-sheet fluorescent imaging (LSFI) to establish rapid reconstruction of the 3-D zebrafish cardiac architecture in response to doxorubicin-induced injury and repair. Input images underwent a 4-step automated image segmentation process consisting of stationary noise removal, histogram equalization, adaptive thresholding, and image fusion followed by 3-D reconstruction. We applied this method to 3-month old zebrafish injected intraperitoneally with doxorubicin followed by LSFI at 3, 30, and 60 days post-injection. We observed an initial decrease in myocardial and endocardial cavity volumes at day 3, followed by ventricular remodeling at day 30, and recovery at day 60 (P < 0.05, n = 7–19). Doxorubicin-injected fish developed ventricular diastolic dysfunction and worsening global cardiac function evidenced by elevated E/A ratios and myocardial performance indexes quantified by pulsed-wave Doppler ultrasound at day 30, followed by normalization at day 60 (P < 0.05, n = 9–20). Treatment with the γ-secretase inhibitor, DAPT, to inhibit cleavage and release of Notch Intracellular Domain (NICD) blocked cardiac architectural regeneration and restoration of ventricular function at day 60 (P < 0.05, n = 6–14). Our approach provides a high-throughput model with translational implications for drug discovery and genetic modifiers of chemotherapy-induced cardiomyopathy.René R. Sevag PackardKyung In BaekTyler BeebeNelson JenYichen DingFeng ShiPeng FeiBong Jin KangPo-Heng ChenJonathan GauMichael ChenJonathan Y. TangYu-Huan ShihYonghe DingDebiao LiXiaolei XuTzung K. HsiaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017) |
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Medicine R Science Q |
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Medicine R Science Q René R. Sevag Packard Kyung In Baek Tyler Beebe Nelson Jen Yichen Ding Feng Shi Peng Fei Bong Jin Kang Po-Heng Chen Jonathan Gau Michael Chen Jonathan Y. Tang Yu-Huan Shih Yonghe Ding Debiao Li Xiaolei Xu Tzung K. Hsiai Automated Segmentation of Light-Sheet Fluorescent Imaging to Characterize Experimental Doxorubicin-Induced Cardiac Injury and Repair |
| description |
Abstract This study sought to develop an automated segmentation approach based on histogram analysis of raw axial images acquired by light-sheet fluorescent imaging (LSFI) to establish rapid reconstruction of the 3-D zebrafish cardiac architecture in response to doxorubicin-induced injury and repair. Input images underwent a 4-step automated image segmentation process consisting of stationary noise removal, histogram equalization, adaptive thresholding, and image fusion followed by 3-D reconstruction. We applied this method to 3-month old zebrafish injected intraperitoneally with doxorubicin followed by LSFI at 3, 30, and 60 days post-injection. We observed an initial decrease in myocardial and endocardial cavity volumes at day 3, followed by ventricular remodeling at day 30, and recovery at day 60 (P < 0.05, n = 7–19). Doxorubicin-injected fish developed ventricular diastolic dysfunction and worsening global cardiac function evidenced by elevated E/A ratios and myocardial performance indexes quantified by pulsed-wave Doppler ultrasound at day 30, followed by normalization at day 60 (P < 0.05, n = 9–20). Treatment with the γ-secretase inhibitor, DAPT, to inhibit cleavage and release of Notch Intracellular Domain (NICD) blocked cardiac architectural regeneration and restoration of ventricular function at day 60 (P < 0.05, n = 6–14). Our approach provides a high-throughput model with translational implications for drug discovery and genetic modifiers of chemotherapy-induced cardiomyopathy. |
| format |
article |
| author |
René R. Sevag Packard Kyung In Baek Tyler Beebe Nelson Jen Yichen Ding Feng Shi Peng Fei Bong Jin Kang Po-Heng Chen Jonathan Gau Michael Chen Jonathan Y. Tang Yu-Huan Shih Yonghe Ding Debiao Li Xiaolei Xu Tzung K. Hsiai |
| author_facet |
René R. Sevag Packard Kyung In Baek Tyler Beebe Nelson Jen Yichen Ding Feng Shi Peng Fei Bong Jin Kang Po-Heng Chen Jonathan Gau Michael Chen Jonathan Y. Tang Yu-Huan Shih Yonghe Ding Debiao Li Xiaolei Xu Tzung K. Hsiai |
| author_sort |
René R. Sevag Packard |
| title |
Automated Segmentation of Light-Sheet Fluorescent Imaging to Characterize Experimental Doxorubicin-Induced Cardiac Injury and Repair |
| title_short |
Automated Segmentation of Light-Sheet Fluorescent Imaging to Characterize Experimental Doxorubicin-Induced Cardiac Injury and Repair |
| title_full |
Automated Segmentation of Light-Sheet Fluorescent Imaging to Characterize Experimental Doxorubicin-Induced Cardiac Injury and Repair |
| title_fullStr |
Automated Segmentation of Light-Sheet Fluorescent Imaging to Characterize Experimental Doxorubicin-Induced Cardiac Injury and Repair |
| title_full_unstemmed |
Automated Segmentation of Light-Sheet Fluorescent Imaging to Characterize Experimental Doxorubicin-Induced Cardiac Injury and Repair |
| title_sort |
automated segmentation of light-sheet fluorescent imaging to characterize experimental doxorubicin-induced cardiac injury and repair |
| publisher |
Nature Portfolio |
| publishDate |
2017 |
| url |
https://doaj.org/article/cf8a7b60f3f1442096de0430ac596c00 |
| work_keys_str_mv |
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| _version_ |
1718395485869309952 |