Effect of Phytopreparations Based on Bioreactor-Grown Cell Biomass of <i>Dioscorea deltoidea</i>, <i>Tribulus terrestris</i> and <i>Panax japonicus</i> on Carbohydrate and Lipid Metabolism in Type 2 Diabetes Mellitus
In the present study, we explored the therapeutic potential of bioreactor-grown cell cultures of the medicinal plant species <i>Dioscorea deltoidea</i>, <i>Tribulus terrestris</i> and <i>Panax japonicus</i> to treat carbohydrate metabolism disorders (CMDs) in labo...
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2021
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oai:doaj.org-article:cf9345744b0e420bbd9daf7937d9e6c82021-11-25T18:34:20ZEffect of Phytopreparations Based on Bioreactor-Grown Cell Biomass of <i>Dioscorea deltoidea</i>, <i>Tribulus terrestris</i> and <i>Panax japonicus</i> on Carbohydrate and Lipid Metabolism in Type 2 Diabetes Mellitus10.3390/nu131138112072-6643https://doaj.org/article/cf9345744b0e420bbd9daf7937d9e6c82021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6643/13/11/3811https://doaj.org/toc/2072-6643In the present study, we explored the therapeutic potential of bioreactor-grown cell cultures of the medicinal plant species <i>Dioscorea deltoidea</i>, <i>Tribulus terrestris</i> and <i>Panax japonicus</i> to treat carbohydrate metabolism disorders (CMDs) in laboratory rats. In the adrenaline model of hyperglycemia, aqueous suspensions of cell biomass pre-administered at a dose of 100 mg dry biomass/kg significantly reduced glucose level in animal blood 1–2.5 h (<i>D. deltoidea</i> and <i>T. terrestris</i>) or 1 h (<i>P. japonicus</i>) after adrenaline hydrochloride administration. In a streptozotocin-induced model of type 2 diabetes mellitus, the cell biomass of <i>D. deltoidea</i> and <i>T. terrestris</i> acted towards normalization of carbohydrate and lipid metabolism, as evidenced by a significant reduction of daily diuresis (by 39–57%), blood-glucose level (by 46–51%), blood content in urine (by 78–80%) and total cholesterol (25–36%) compared to animals without treatment. Bioactive secondary metabolites identified in the cell cultures and potentially responsible for their actions were deltoside, 25(S)-protodioscin and protodioscin in <i>D. deltoidea</i>; furostanol-type steroidal glycosides and quinic acid derivatives in <i>T. terrestris</i>; and ginsenosides and malonyl-ginsenosides in <i>P. japonicus.</i> These results evidenced for high potential of bioreactor-grown cell suspensions of these species for prevention and treatment of CMD, which requires further investigation.Maria N. PovydyshMaria V. TitovaIgor M. IvanovAndrey G. KlushinDmitry V. KochkinBoris A. GalishevElena V. PopovaDmitry Yu. IvkinVladimir G. LuzhaninMarina V. KrasnovaNatalia V. DemakovaAlexander M. NosovMDPI AGarticleadrenaline modelbioreactorsfurostanol-type glycosideshyperglycemiaplant cell culturestreptozotocin-induced type 2 diabetes mellitusNutrition. Foods and food supplyTX341-641ENNutrients, Vol 13, Iss 3811, p 3811 (2021) |
| institution |
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| collection |
DOAJ |
| language |
EN |
| topic |
adrenaline model bioreactors furostanol-type glycosides hyperglycemia plant cell culture streptozotocin-induced type 2 diabetes mellitus Nutrition. Foods and food supply TX341-641 |
| spellingShingle |
adrenaline model bioreactors furostanol-type glycosides hyperglycemia plant cell culture streptozotocin-induced type 2 diabetes mellitus Nutrition. Foods and food supply TX341-641 Maria N. Povydysh Maria V. Titova Igor M. Ivanov Andrey G. Klushin Dmitry V. Kochkin Boris A. Galishev Elena V. Popova Dmitry Yu. Ivkin Vladimir G. Luzhanin Marina V. Krasnova Natalia V. Demakova Alexander M. Nosov Effect of Phytopreparations Based on Bioreactor-Grown Cell Biomass of <i>Dioscorea deltoidea</i>, <i>Tribulus terrestris</i> and <i>Panax japonicus</i> on Carbohydrate and Lipid Metabolism in Type 2 Diabetes Mellitus |
| description |
In the present study, we explored the therapeutic potential of bioreactor-grown cell cultures of the medicinal plant species <i>Dioscorea deltoidea</i>, <i>Tribulus terrestris</i> and <i>Panax japonicus</i> to treat carbohydrate metabolism disorders (CMDs) in laboratory rats. In the adrenaline model of hyperglycemia, aqueous suspensions of cell biomass pre-administered at a dose of 100 mg dry biomass/kg significantly reduced glucose level in animal blood 1–2.5 h (<i>D. deltoidea</i> and <i>T. terrestris</i>) or 1 h (<i>P. japonicus</i>) after adrenaline hydrochloride administration. In a streptozotocin-induced model of type 2 diabetes mellitus, the cell biomass of <i>D. deltoidea</i> and <i>T. terrestris</i> acted towards normalization of carbohydrate and lipid metabolism, as evidenced by a significant reduction of daily diuresis (by 39–57%), blood-glucose level (by 46–51%), blood content in urine (by 78–80%) and total cholesterol (25–36%) compared to animals without treatment. Bioactive secondary metabolites identified in the cell cultures and potentially responsible for their actions were deltoside, 25(S)-protodioscin and protodioscin in <i>D. deltoidea</i>; furostanol-type steroidal glycosides and quinic acid derivatives in <i>T. terrestris</i>; and ginsenosides and malonyl-ginsenosides in <i>P. japonicus.</i> These results evidenced for high potential of bioreactor-grown cell suspensions of these species for prevention and treatment of CMD, which requires further investigation. |
| format |
article |
| author |
Maria N. Povydysh Maria V. Titova Igor M. Ivanov Andrey G. Klushin Dmitry V. Kochkin Boris A. Galishev Elena V. Popova Dmitry Yu. Ivkin Vladimir G. Luzhanin Marina V. Krasnova Natalia V. Demakova Alexander M. Nosov |
| author_facet |
Maria N. Povydysh Maria V. Titova Igor M. Ivanov Andrey G. Klushin Dmitry V. Kochkin Boris A. Galishev Elena V. Popova Dmitry Yu. Ivkin Vladimir G. Luzhanin Marina V. Krasnova Natalia V. Demakova Alexander M. Nosov |
| author_sort |
Maria N. Povydysh |
| title |
Effect of Phytopreparations Based on Bioreactor-Grown Cell Biomass of <i>Dioscorea deltoidea</i>, <i>Tribulus terrestris</i> and <i>Panax japonicus</i> on Carbohydrate and Lipid Metabolism in Type 2 Diabetes Mellitus |
| title_short |
Effect of Phytopreparations Based on Bioreactor-Grown Cell Biomass of <i>Dioscorea deltoidea</i>, <i>Tribulus terrestris</i> and <i>Panax japonicus</i> on Carbohydrate and Lipid Metabolism in Type 2 Diabetes Mellitus |
| title_full |
Effect of Phytopreparations Based on Bioreactor-Grown Cell Biomass of <i>Dioscorea deltoidea</i>, <i>Tribulus terrestris</i> and <i>Panax japonicus</i> on Carbohydrate and Lipid Metabolism in Type 2 Diabetes Mellitus |
| title_fullStr |
Effect of Phytopreparations Based on Bioreactor-Grown Cell Biomass of <i>Dioscorea deltoidea</i>, <i>Tribulus terrestris</i> and <i>Panax japonicus</i> on Carbohydrate and Lipid Metabolism in Type 2 Diabetes Mellitus |
| title_full_unstemmed |
Effect of Phytopreparations Based on Bioreactor-Grown Cell Biomass of <i>Dioscorea deltoidea</i>, <i>Tribulus terrestris</i> and <i>Panax japonicus</i> on Carbohydrate and Lipid Metabolism in Type 2 Diabetes Mellitus |
| title_sort |
effect of phytopreparations based on bioreactor-grown cell biomass of <i>dioscorea deltoidea</i>, <i>tribulus terrestris</i> and <i>panax japonicus</i> on carbohydrate and lipid metabolism in type 2 diabetes mellitus |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/cf9345744b0e420bbd9daf7937d9e6c8 |
| work_keys_str_mv |
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