Proteomic footprint of myocardial ischemia/reperfusion injury: Longitudinal study of the at-risk and remote regions in the pig model

Abstract Reperfusion alters post-myocardial infarction (MI) healing; however, very few systematic studies report the early molecular changes following ischemia/reperfusion (I/R). Alterations in the remote myocardium have also been neglected, disregarding its contribution to post-MI heart failure (HF...

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Autores principales:	Aleksandra Binek, Rodrigo Fernández-Jiménez, Inmaculada Jorge, Emilio Camafeita, Juan Antonio López, Navratan Bagwan, Carlos Galán-Arriola, Andres Pun, Jaume Agüero, Valentin Fuster, Borja Ibanez, Jesús Vázquez
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cf9700c0ebdd447889bd706bbb11a550
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spelling	oai:doaj.org-article:cf9700c0ebdd447889bd706bbb11a5502021-12-02T11:52:22ZProteomic footprint of myocardial ischemia/reperfusion injury: Longitudinal study of the at-risk and remote regions in the pig model10.1038/s41598-017-11985-52045-2322https://doaj.org/article/cf9700c0ebdd447889bd706bbb11a5502017-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-11985-5https://doaj.org/toc/2045-2322Abstract Reperfusion alters post-myocardial infarction (MI) healing; however, very few systematic studies report the early molecular changes following ischemia/reperfusion (I/R). Alterations in the remote myocardium have also been neglected, disregarding its contribution to post-MI heart failure (HF) development. This study characterizes protein dynamics and contractile abnormalities in the ischemic and remote myocardium during one week after MI. Closed-chest 40 min I/R was performed in 20 pigs sacrificed at 120 min, 24 hours, 4days, and 7days after reperfusion (n = 5 per group). Myocardial contractility was followed up by cardiac magnetic resonance (CMR) and tissue samples were analyzed by multiplexed quantitative proteomics. At early reperfusion (120 min), the ischemic area showed a coordinated upregulation of inflammatory processes, whereas interstitial proteins, angiogenesis and cardio-renal signaling processes increased at later reperfusion (day 4 and 7). Remote myocardium showed decreased contractility at 120 min- and 24 h-CMR accompanied by transient alterations in contractile and mitochondrial proteins. Subsequent recovery of regional contractility was associated with edema formation on CMR and increases in inflammation and wound healing proteins on post-MI day 7. Our results establish for the first time the altered protein signatures in the ischemic and remote myocardium early after I/R and might have implications for new therapeutic targets to improve early post-MI remodeling.Aleksandra BinekRodrigo Fernández-JiménezInmaculada JorgeEmilio CamafeitaJuan Antonio LópezNavratan BagwanCarlos Galán-ArriolaAndres PunJaume AgüeroValentin FusterBorja IbanezJesús VázquezNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-16 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aleksandra Binek Rodrigo Fernández-Jiménez Inmaculada Jorge Emilio Camafeita Juan Antonio López Navratan Bagwan Carlos Galán-Arriola Andres Pun Jaume Agüero Valentin Fuster Borja Ibanez Jesús Vázquez Proteomic footprint of myocardial ischemia/reperfusion injury: Longitudinal study of the at-risk and remote regions in the pig model
description	Abstract Reperfusion alters post-myocardial infarction (MI) healing; however, very few systematic studies report the early molecular changes following ischemia/reperfusion (I/R). Alterations in the remote myocardium have also been neglected, disregarding its contribution to post-MI heart failure (HF) development. This study characterizes protein dynamics and contractile abnormalities in the ischemic and remote myocardium during one week after MI. Closed-chest 40 min I/R was performed in 20 pigs sacrificed at 120 min, 24 hours, 4days, and 7days after reperfusion (n = 5 per group). Myocardial contractility was followed up by cardiac magnetic resonance (CMR) and tissue samples were analyzed by multiplexed quantitative proteomics. At early reperfusion (120 min), the ischemic area showed a coordinated upregulation of inflammatory processes, whereas interstitial proteins, angiogenesis and cardio-renal signaling processes increased at later reperfusion (day 4 and 7). Remote myocardium showed decreased contractility at 120 min- and 24 h-CMR accompanied by transient alterations in contractile and mitochondrial proteins. Subsequent recovery of regional contractility was associated with edema formation on CMR and increases in inflammation and wound healing proteins on post-MI day 7. Our results establish for the first time the altered protein signatures in the ischemic and remote myocardium early after I/R and might have implications for new therapeutic targets to improve early post-MI remodeling.
format	article
author	Aleksandra Binek Rodrigo Fernández-Jiménez Inmaculada Jorge Emilio Camafeita Juan Antonio López Navratan Bagwan Carlos Galán-Arriola Andres Pun Jaume Agüero Valentin Fuster Borja Ibanez Jesús Vázquez
author_facet	Aleksandra Binek Rodrigo Fernández-Jiménez Inmaculada Jorge Emilio Camafeita Juan Antonio López Navratan Bagwan Carlos Galán-Arriola Andres Pun Jaume Agüero Valentin Fuster Borja Ibanez Jesús Vázquez
author_sort	Aleksandra Binek
title	Proteomic footprint of myocardial ischemia/reperfusion injury: Longitudinal study of the at-risk and remote regions in the pig model
title_short	Proteomic footprint of myocardial ischemia/reperfusion injury: Longitudinal study of the at-risk and remote regions in the pig model
title_full	Proteomic footprint of myocardial ischemia/reperfusion injury: Longitudinal study of the at-risk and remote regions in the pig model
title_fullStr	Proteomic footprint of myocardial ischemia/reperfusion injury: Longitudinal study of the at-risk and remote regions in the pig model
title_full_unstemmed	Proteomic footprint of myocardial ischemia/reperfusion injury: Longitudinal study of the at-risk and remote regions in the pig model
title_sort	proteomic footprint of myocardial ischemia/reperfusion injury: longitudinal study of the at-risk and remote regions in the pig model
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cf9700c0ebdd447889bd706bbb11a550
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Proteomic footprint of myocardial ischemia/reperfusion injury: Longitudinal study of the at-risk and remote regions in the pig model

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