Developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos

Abstract During preimplantation development, mammalian embryo cells (blastomeres) cleave, gradually losing their potencies and differentiating into three primary cell lineages: epiblast (EPI), trophectoderm (TE), and primitive endoderm (PE). The exact moment at which cells begin to vary in their pot...

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Autores principales:	Katarzyna Krawczyk, Ewa Kosyl, Karolina Częścik-Łysyszyn, Tomasz Wyszomirski, Marek Maleszewski
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cf9fab2c21564696a47c4911b4a7e51b
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spelling	oai:doaj.org-article:cf9fab2c21564696a47c4911b4a7e51b2021-11-08T10:55:59ZDevelopmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos10.1038/s41598-021-00834-12045-2322https://doaj.org/article/cf9fab2c21564696a47c4911b4a7e51b2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-00834-1https://doaj.org/toc/2045-2322Abstract During preimplantation development, mammalian embryo cells (blastomeres) cleave, gradually losing their potencies and differentiating into three primary cell lineages: epiblast (EPI), trophectoderm (TE), and primitive endoderm (PE). The exact moment at which cells begin to vary in their potency for multilineage differentiation still remains unknown. We sought to answer the question of whether single cells isolated from 2- and 4-cell embryos differ in their ability to generate the progenitors and cells of blastocyst lineages. We revealed that twins were often able to develop into blastocysts containing inner cell masses (ICMs) with PE and EPI cells. Despite their capacity to create a blastocyst, the twins differed in their ability to produce EPI, PE, and TE cell lineages. In contrast, quadruplets rarely formed normal blastocysts, but instead developed into blastocysts with ICMs composed of only one cell lineage or completely devoid of an ICM altogether. We also showed that quadruplets have unequal capacities to differentiate into TE, PE, and EPI lineages. These findings could explain the difficulty of creating monozygotic twins and quadruplets from 2- and 4-cell stage mouse embryos.Katarzyna KrawczykEwa KosylKarolina Częścik-ŁysyszynTomasz WyszomirskiMarek MaleszewskiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Katarzyna Krawczyk Ewa Kosyl Karolina Częścik-Łysyszyn Tomasz Wyszomirski Marek Maleszewski Developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos
description	Abstract During preimplantation development, mammalian embryo cells (blastomeres) cleave, gradually losing their potencies and differentiating into three primary cell lineages: epiblast (EPI), trophectoderm (TE), and primitive endoderm (PE). The exact moment at which cells begin to vary in their potency for multilineage differentiation still remains unknown. We sought to answer the question of whether single cells isolated from 2- and 4-cell embryos differ in their ability to generate the progenitors and cells of blastocyst lineages. We revealed that twins were often able to develop into blastocysts containing inner cell masses (ICMs) with PE and EPI cells. Despite their capacity to create a blastocyst, the twins differed in their ability to produce EPI, PE, and TE cell lineages. In contrast, quadruplets rarely formed normal blastocysts, but instead developed into blastocysts with ICMs composed of only one cell lineage or completely devoid of an ICM altogether. We also showed that quadruplets have unequal capacities to differentiate into TE, PE, and EPI lineages. These findings could explain the difficulty of creating monozygotic twins and quadruplets from 2- and 4-cell stage mouse embryos.
format	article
author	Katarzyna Krawczyk Ewa Kosyl Karolina Częścik-Łysyszyn Tomasz Wyszomirski Marek Maleszewski
author_facet	Katarzyna Krawczyk Ewa Kosyl Karolina Częścik-Łysyszyn Tomasz Wyszomirski Marek Maleszewski
author_sort	Katarzyna Krawczyk
title	Developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos
title_short	Developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos
title_full	Developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos
title_fullStr	Developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos
title_full_unstemmed	Developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos
title_sort	developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cf9fab2c21564696a47c4911b4a7e51b
work_keys_str_mv	AT katarzynakrawczyk developmentalcapacityisunevenlydistributedamongsingleblastomeresof2celland4cellstagemouseembryos AT ewakosyl developmentalcapacityisunevenlydistributedamongsingleblastomeresof2celland4cellstagemouseembryos AT karolinaczescikłysyszyn developmentalcapacityisunevenlydistributedamongsingleblastomeresof2celland4cellstagemouseembryos AT tomaszwyszomirski developmentalcapacityisunevenlydistributedamongsingleblastomeresof2celland4cellstagemouseembryos AT marekmaleszewski developmentalcapacityisunevenlydistributedamongsingleblastomeresof2celland4cellstagemouseembryos
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Developmental capacity is unevenly distributed among single blastomeres of 2-cell and 4-cell stage mouse embryos

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