The FtcR-Like Protein ActR in Azorhizobium caulinodans ORS571 Is Involved in Bacterial Motility and Symbiosis With the Host Plant

Bacterial signal transduction pathways are important for a variety of adaptive responses to environment, such as two-component systems (TCSs). In this paper, we reported the characterization of a transcriptional regulator in Azorhizobium caulinodans ORS571, ActR, with an N-terminal receiver domain a...

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Autores principales:	Li Sun, Dandan Wang, Zhiqiu Yin, Chengsheng Zhang, Amber Bible, Zhihong Xie
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Azorhizobium caulinodans ORS571 transcriptional regulator flagella exopolysaccharide biofilm symbiosis Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/cfa0ddb66eae4733874024a7fb5a53b6
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spelling	oai:doaj.org-article:cfa0ddb66eae4733874024a7fb5a53b62021-11-19T04:54:02ZThe FtcR-Like Protein ActR in Azorhizobium caulinodans ORS571 Is Involved in Bacterial Motility and Symbiosis With the Host Plant1664-302X10.3389/fmicb.2021.744268https://doaj.org/article/cfa0ddb66eae4733874024a7fb5a53b62021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.744268/fullhttps://doaj.org/toc/1664-302XBacterial signal transduction pathways are important for a variety of adaptive responses to environment, such as two-component systems (TCSs). In this paper, we reported the characterization of a transcriptional regulator in Azorhizobium caulinodans ORS571, ActR, with an N-terminal receiver domain and one C-terminal OmpR/PhoB-type DNA binding domain. Sequence analysis showed that ActR shared a high similarity with FtcR regulator of Brucella melitensis 16M known to be involved in flagellar regulation. The structural gene of this regulator was largely distributed in Alphaproteobacteria, in particular in Rhizobiales and Rhodobacterales, and was located within clusters of genes related to motility functions. Furthermore, we studied the biological function of ActR in A. caulinodans grown at the free-living state or in association with Sesbania rostrata by constructing actR gene deletion mutant. In the free-living state, the bacterial flagellum and motility ability were entirely deleted, the expression of flagellar genes was downregulated; and the exopolysaccharide production, biofilm formation, and cell flocculation decreased significantly compared with those of the wild-type strain. In the symbiotic state, ΔactR mutant strain showed weakly competitive colonization and nodulation on the host plant. These results illustrated that FtcR-like regulator in A. caulinodans is involved in flagellar biosynthesis and provide bacteria with an effective competitive nodulation for symbiosis. These findings improved our knowledge of FtcR-like transcriptional regulator in A. caulinodans.Li SunDandan WangZhiqiu YinChengsheng ZhangAmber BibleZhihong XieZhihong XieFrontiers Media S.A.articleAzorhizobium caulinodans ORS571transcriptional regulatorflagellaexopolysaccharidebiofilmsymbiosisMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Azorhizobium caulinodans ORS571 transcriptional regulator flagella exopolysaccharide biofilm symbiosis Microbiology QR1-502
spellingShingle	Azorhizobium caulinodans ORS571 transcriptional regulator flagella exopolysaccharide biofilm symbiosis Microbiology QR1-502 Li Sun Dandan Wang Zhiqiu Yin Chengsheng Zhang Amber Bible Zhihong Xie Zhihong Xie The FtcR-Like Protein ActR in Azorhizobium caulinodans ORS571 Is Involved in Bacterial Motility and Symbiosis With the Host Plant
description	Bacterial signal transduction pathways are important for a variety of adaptive responses to environment, such as two-component systems (TCSs). In this paper, we reported the characterization of a transcriptional regulator in Azorhizobium caulinodans ORS571, ActR, with an N-terminal receiver domain and one C-terminal OmpR/PhoB-type DNA binding domain. Sequence analysis showed that ActR shared a high similarity with FtcR regulator of Brucella melitensis 16M known to be involved in flagellar regulation. The structural gene of this regulator was largely distributed in Alphaproteobacteria, in particular in Rhizobiales and Rhodobacterales, and was located within clusters of genes related to motility functions. Furthermore, we studied the biological function of ActR in A. caulinodans grown at the free-living state or in association with Sesbania rostrata by constructing actR gene deletion mutant. In the free-living state, the bacterial flagellum and motility ability were entirely deleted, the expression of flagellar genes was downregulated; and the exopolysaccharide production, biofilm formation, and cell flocculation decreased significantly compared with those of the wild-type strain. In the symbiotic state, ΔactR mutant strain showed weakly competitive colonization and nodulation on the host plant. These results illustrated that FtcR-like regulator in A. caulinodans is involved in flagellar biosynthesis and provide bacteria with an effective competitive nodulation for symbiosis. These findings improved our knowledge of FtcR-like transcriptional regulator in A. caulinodans.
format	article
author	Li Sun Dandan Wang Zhiqiu Yin Chengsheng Zhang Amber Bible Zhihong Xie Zhihong Xie
author_facet	Li Sun Dandan Wang Zhiqiu Yin Chengsheng Zhang Amber Bible Zhihong Xie Zhihong Xie
author_sort	Li Sun
title	The FtcR-Like Protein ActR in Azorhizobium caulinodans ORS571 Is Involved in Bacterial Motility and Symbiosis With the Host Plant
title_short	The FtcR-Like Protein ActR in Azorhizobium caulinodans ORS571 Is Involved in Bacterial Motility and Symbiosis With the Host Plant
title_full	The FtcR-Like Protein ActR in Azorhizobium caulinodans ORS571 Is Involved in Bacterial Motility and Symbiosis With the Host Plant
title_fullStr	The FtcR-Like Protein ActR in Azorhizobium caulinodans ORS571 Is Involved in Bacterial Motility and Symbiosis With the Host Plant
title_full_unstemmed	The FtcR-Like Protein ActR in Azorhizobium caulinodans ORS571 Is Involved in Bacterial Motility and Symbiosis With the Host Plant
title_sort	ftcr-like protein actr in azorhizobium caulinodans ors571 is involved in bacterial motility and symbiosis with the host plant
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/cfa0ddb66eae4733874024a7fb5a53b6
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The FtcR-Like Protein ActR in Azorhizobium caulinodans ORS571 Is Involved in Bacterial Motility and Symbiosis With the Host Plant

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