Standardization of Diploid Codonopsis laceolata Root Extract as an Anti-Hyperuricemic Source

Codonopsis lanceolate exerts various medicinal effects and has been used as a traditional medicine for inflammation, asthma, gastritis, and liver disease. Recently, we reported the xanthine oxidase inhibitory activity of C. lanceolata extract and that lobetyolin...

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Main Authors:	Seung-Yub Song, So-Hyeon Bok, Sung-Ho Lee, Min-Hee Kim, Hee-Ock Boo, Hak-Hyun Kim, Dae-Hun Park, Seung-Sik Cho
Format:	article
Language:	EN
Published:	MDPI AG 2021
Subjects:	<i>C. lanceolata</i> extraction optimization xanthine oxidase hyperuricemia Chemical technology TP1-1185 Chemistry QD1-999
Online Access:	https://doaj.org/article/cfa321f6200842aeb2d6540a9fc73fb6
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spelling	oai:doaj.org-article:cfa321f6200842aeb2d6540a9fc73fb62021-11-25T18:51:55ZStandardization of Diploid <i>Codonopsis laceolata</i> Root Extract as an Anti-Hyperuricemic Source10.3390/pr91120652227-9717https://doaj.org/article/cfa321f6200842aeb2d6540a9fc73fb62021-11-01T00:00:00Zhttps://www.mdpi.com/2227-9717/9/11/2065https://doaj.org/toc/2227-9717<i>Codonopsis lanceolate</i> exerts various medicinal effects and has been used as a traditional medicine for inflammation, asthma, gastritis, and liver disease. Recently, we reported the xanthine oxidase inhibitory activity of <i>C. lanceolata</i> extract and that lobetyolin, one of the key components, was a xanthine oxidase inhibitor. Lobetyolin showed anti-hyperuricemic activity in vitro and in vivo. In this study, we prepared various types of <i>C. lanceolata</i> extracts for the development of functional materials and natural drugs. We present the optimal analytical approach for the quality control and extraction optimization of <i>C. lanceolata</i> preparations. We established and validated a HPLC analysis for easy separation and quantification of the lobetyolin biomarker. Solvent extracts of <i>C. lanceolata</i> root were prepared and the profiles of the active marker and the optimal extraction methods were evaluated. The 100% ethanolic extract demonstrated the highest lobetyolin content. The validated HPLC method confirmed that lobetyolin was present in <i>C. lanceolata</i> root extracts. We suggest that the anti-hyperuricemic activities of <i>C. lanceolata</i> extract could be attributed to this marker compound. The results proposed that the 100% ethanolic extract could be used for the prevention of hyperurecemia, and that this analytical method and biomarker could be useful for the quality control of <i>C. lanceolata</i> preparations.Seung-Yub SongSo-Hyeon BokSung-Ho LeeMin-Hee KimHee-Ock BooHak-Hyun KimDae-Hun ParkSeung-Sik ChoMDPI AGarticle<i>C. lanceolata</i>extraction optimizationxanthine oxidasehyperuricemiaChemical technologyTP1-1185ChemistryQD1-999ENProcesses, Vol 9, Iss 2065, p 2065 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>C. lanceolata</i> extraction optimization xanthine oxidase hyperuricemia Chemical technology TP1-1185 Chemistry QD1-999
spellingShingle	<i>C. lanceolata</i> extraction optimization xanthine oxidase hyperuricemia Chemical technology TP1-1185 Chemistry QD1-999 Seung-Yub Song So-Hyeon Bok Sung-Ho Lee Min-Hee Kim Hee-Ock Boo Hak-Hyun Kim Dae-Hun Park Seung-Sik Cho Standardization of Diploid <i>Codonopsis laceolata</i> Root Extract as an Anti-Hyperuricemic Source
description	<i>Codonopsis lanceolate</i> exerts various medicinal effects and has been used as a traditional medicine for inflammation, asthma, gastritis, and liver disease. Recently, we reported the xanthine oxidase inhibitory activity of <i>C. lanceolata</i> extract and that lobetyolin, one of the key components, was a xanthine oxidase inhibitor. Lobetyolin showed anti-hyperuricemic activity in vitro and in vivo. In this study, we prepared various types of <i>C. lanceolata</i> extracts for the development of functional materials and natural drugs. We present the optimal analytical approach for the quality control and extraction optimization of <i>C. lanceolata</i> preparations. We established and validated a HPLC analysis for easy separation and quantification of the lobetyolin biomarker. Solvent extracts of <i>C. lanceolata</i> root were prepared and the profiles of the active marker and the optimal extraction methods were evaluated. The 100% ethanolic extract demonstrated the highest lobetyolin content. The validated HPLC method confirmed that lobetyolin was present in <i>C. lanceolata</i> root extracts. We suggest that the anti-hyperuricemic activities of <i>C. lanceolata</i> extract could be attributed to this marker compound. The results proposed that the 100% ethanolic extract could be used for the prevention of hyperurecemia, and that this analytical method and biomarker could be useful for the quality control of <i>C. lanceolata</i> preparations.
format	article
author	Seung-Yub Song So-Hyeon Bok Sung-Ho Lee Min-Hee Kim Hee-Ock Boo Hak-Hyun Kim Dae-Hun Park Seung-Sik Cho
author_facet	Seung-Yub Song So-Hyeon Bok Sung-Ho Lee Min-Hee Kim Hee-Ock Boo Hak-Hyun Kim Dae-Hun Park Seung-Sik Cho
author_sort	Seung-Yub Song
title	Standardization of Diploid <i>Codonopsis laceolata</i> Root Extract as an Anti-Hyperuricemic Source
title_short	Standardization of Diploid <i>Codonopsis laceolata</i> Root Extract as an Anti-Hyperuricemic Source
title_full	Standardization of Diploid <i>Codonopsis laceolata</i> Root Extract as an Anti-Hyperuricemic Source
title_fullStr	Standardization of Diploid <i>Codonopsis laceolata</i> Root Extract as an Anti-Hyperuricemic Source
title_full_unstemmed	Standardization of Diploid <i>Codonopsis laceolata</i> Root Extract as an Anti-Hyperuricemic Source
title_sort	standardization of diploid <i>codonopsis laceolata</i> root extract as an anti-hyperuricemic source
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cfa321f6200842aeb2d6540a9fc73fb6
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_version_	1718410619742322688

Standardization of Diploid <i>Codonopsis laceolata</i> Root Extract as an Anti-Hyperuricemic Source

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