Full-length minor ampullate spidroin gene sequence.

Spider silk includes seven protein based fibers and glue-like substances produced by glands in the spider's abdomen. Minor ampullate silk is used to make the auxiliary spiral of the orb-web and also for wrapping prey, has a high tensile strength and does not supercontract in water. So far, only...

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Autores principales:	Gefei Chen, Xiangqin Liu, Yunlong Zhang, Senzhu Lin, Zijiang Yang, Jan Johansson, Anna Rising, Qing Meng
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cfa5029df4dc47c183083390307d9aec
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spelling	oai:doaj.org-article:cfa5029df4dc47c183083390307d9aec2021-11-18T08:05:00ZFull-length minor ampullate spidroin gene sequence.1932-620310.1371/journal.pone.0052293https://doaj.org/article/cfa5029df4dc47c183083390307d9aec2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23251707/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Spider silk includes seven protein based fibers and glue-like substances produced by glands in the spider's abdomen. Minor ampullate silk is used to make the auxiliary spiral of the orb-web and also for wrapping prey, has a high tensile strength and does not supercontract in water. So far, only partial cDNA sequences have been obtained for minor ampullate spidroins (MiSps). Here we describe the first MiSp full-length gene sequence from the spider species Araneus ventricosus, using a multidimensional PCR approach. Comparative analysis of the sequence reveals regulatory elements, as well as unique spidroin gene and protein architecture including the presence of an unusually large intron. The spliced full-length transcript of MiSp gene is 5440 bp in size and encodes 1766 amino acid residues organized into conserved nonrepetitive N- and C-terminal domains and a central predominantly repetitive region composed of four units that are iterated in a non regular manner. The repeats are more conserved within A. ventricosus MiSp than compared to repeats from homologous proteins, and are interrupted by two nonrepetitive spacer regions, which have 100% identity even at the nucleotide level.Gefei ChenXiangqin LiuYunlong ZhangSenzhu LinZijiang YangJan JohanssonAnna RisingQing MengPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e52293 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Gefei Chen Xiangqin Liu Yunlong Zhang Senzhu Lin Zijiang Yang Jan Johansson Anna Rising Qing Meng Full-length minor ampullate spidroin gene sequence.
description	Spider silk includes seven protein based fibers and glue-like substances produced by glands in the spider's abdomen. Minor ampullate silk is used to make the auxiliary spiral of the orb-web and also for wrapping prey, has a high tensile strength and does not supercontract in water. So far, only partial cDNA sequences have been obtained for minor ampullate spidroins (MiSps). Here we describe the first MiSp full-length gene sequence from the spider species Araneus ventricosus, using a multidimensional PCR approach. Comparative analysis of the sequence reveals regulatory elements, as well as unique spidroin gene and protein architecture including the presence of an unusually large intron. The spliced full-length transcript of MiSp gene is 5440 bp in size and encodes 1766 amino acid residues organized into conserved nonrepetitive N- and C-terminal domains and a central predominantly repetitive region composed of four units that are iterated in a non regular manner. The repeats are more conserved within A. ventricosus MiSp than compared to repeats from homologous proteins, and are interrupted by two nonrepetitive spacer regions, which have 100% identity even at the nucleotide level.
format	article
author	Gefei Chen Xiangqin Liu Yunlong Zhang Senzhu Lin Zijiang Yang Jan Johansson Anna Rising Qing Meng
author_facet	Gefei Chen Xiangqin Liu Yunlong Zhang Senzhu Lin Zijiang Yang Jan Johansson Anna Rising Qing Meng
author_sort	Gefei Chen
title	Full-length minor ampullate spidroin gene sequence.
title_short	Full-length minor ampullate spidroin gene sequence.
title_full	Full-length minor ampullate spidroin gene sequence.
title_fullStr	Full-length minor ampullate spidroin gene sequence.
title_full_unstemmed	Full-length minor ampullate spidroin gene sequence.
title_sort	full-length minor ampullate spidroin gene sequence.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/cfa5029df4dc47c183083390307d9aec
work_keys_str_mv	AT gefeichen fulllengthminorampullatespidroingenesequence AT xiangqinliu fulllengthminorampullatespidroingenesequence AT yunlongzhang fulllengthminorampullatespidroingenesequence AT senzhulin fulllengthminorampullatespidroingenesequence AT zijiangyang fulllengthminorampullatespidroingenesequence AT janjohansson fulllengthminorampullatespidroingenesequence AT annarising fulllengthminorampullatespidroingenesequence AT qingmeng fulllengthminorampullatespidroingenesequence
_version_	1718422258365497344

Full-length minor ampullate spidroin gene sequence.

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