Extraction and Purification of R-Phycoerythrin Alpha Subunit from the Marine Red Algae <i>Pyropia Yezoensis</i> and Its Biological Activities

Phycoerythrin is a major light-harvesting pigment of red algae and cyanobacteria that is widely used as a fluorescent probe or as a colorant in the food and cosmetic industries. In this study, phycoerythrin was extracted from the red algae <i>Pyropia yezoensis</i> and purified by ammoniu...

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Autores principales:	Selvakumari Ulagesan, Taek-Jeong Nam, Youn-Hee Choi
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Pyropia yezoensis</i> marine algae phycoerythrin antioxidant activity cytotoxicity anticancer activity Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/cfa64139523249c682ea921ad111ff3c
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spelling	oai:doaj.org-article:cfa64139523249c682ea921ad111ff3c2021-11-11T18:29:18ZExtraction and Purification of R-Phycoerythrin Alpha Subunit from the Marine Red Algae <i>Pyropia Yezoensis</i> and Its Biological Activities10.3390/molecules262164791420-3049https://doaj.org/article/cfa64139523249c682ea921ad111ff3c2021-10-01T00:00:00Zhttps://www.mdpi.com/1420-3049/26/21/6479https://doaj.org/toc/1420-3049Phycoerythrin is a major light-harvesting pigment of red algae and cyanobacteria that is widely used as a fluorescent probe or as a colorant in the food and cosmetic industries. In this study, phycoerythrin was extracted from the red algae <i>Pyropia yezoensis</i> and purified by ammonium sulfate precipitation and various chromatography methods. The purified phycoerythrin was analyzed by UV-visible and fluorescence spectroscopy. The isolated pigment had the typical spectrum of R-phycoerythrin, with a trimmer state with absorbance maxima at 497, 536, and 565 nm. It was further purified and identified by LC-MS/MS and Mascot search. It showed a 100% sequence similarity with the R-phycoerythrin alpha subunit of <i>Pyropia yezoensis</i>. The molecular mass was 17.97 kDa. The antioxidant activity of the purified R-phycoerythrin alpha subunit was analyzed. It showed significant antioxidant activity in ABTS and FRAP assays and had significant cytotoxicity against HepG2 cells.Selvakumari UlagesanTaek-Jeong NamYoun-Hee ChoiMDPI AGarticle<i>Pyropia yezoensis</i>marine algaephycoerythrinantioxidant activitycytotoxicityanticancer activityOrganic chemistryQD241-441ENMolecules, Vol 26, Iss 6479, p 6479 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Pyropia yezoensis</i> marine algae phycoerythrin antioxidant activity cytotoxicity anticancer activity Organic chemistry QD241-441
spellingShingle	<i>Pyropia yezoensis</i> marine algae phycoerythrin antioxidant activity cytotoxicity anticancer activity Organic chemistry QD241-441 Selvakumari Ulagesan Taek-Jeong Nam Youn-Hee Choi Extraction and Purification of R-Phycoerythrin Alpha Subunit from the Marine Red Algae <i>Pyropia Yezoensis</i> and Its Biological Activities
description	Phycoerythrin is a major light-harvesting pigment of red algae and cyanobacteria that is widely used as a fluorescent probe or as a colorant in the food and cosmetic industries. In this study, phycoerythrin was extracted from the red algae <i>Pyropia yezoensis</i> and purified by ammonium sulfate precipitation and various chromatography methods. The purified phycoerythrin was analyzed by UV-visible and fluorescence spectroscopy. The isolated pigment had the typical spectrum of R-phycoerythrin, with a trimmer state with absorbance maxima at 497, 536, and 565 nm. It was further purified and identified by LC-MS/MS and Mascot search. It showed a 100% sequence similarity with the R-phycoerythrin alpha subunit of <i>Pyropia yezoensis</i>. The molecular mass was 17.97 kDa. The antioxidant activity of the purified R-phycoerythrin alpha subunit was analyzed. It showed significant antioxidant activity in ABTS and FRAP assays and had significant cytotoxicity against HepG2 cells.
format	article
author	Selvakumari Ulagesan Taek-Jeong Nam Youn-Hee Choi
author_facet	Selvakumari Ulagesan Taek-Jeong Nam Youn-Hee Choi
author_sort	Selvakumari Ulagesan
title	Extraction and Purification of R-Phycoerythrin Alpha Subunit from the Marine Red Algae <i>Pyropia Yezoensis</i> and Its Biological Activities
title_short	Extraction and Purification of R-Phycoerythrin Alpha Subunit from the Marine Red Algae <i>Pyropia Yezoensis</i> and Its Biological Activities
title_full	Extraction and Purification of R-Phycoerythrin Alpha Subunit from the Marine Red Algae <i>Pyropia Yezoensis</i> and Its Biological Activities
title_fullStr	Extraction and Purification of R-Phycoerythrin Alpha Subunit from the Marine Red Algae <i>Pyropia Yezoensis</i> and Its Biological Activities
title_full_unstemmed	Extraction and Purification of R-Phycoerythrin Alpha Subunit from the Marine Red Algae <i>Pyropia Yezoensis</i> and Its Biological Activities
title_sort	extraction and purification of r-phycoerythrin alpha subunit from the marine red algae <i>pyropia yezoensis</i> and its biological activities
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cfa64139523249c682ea921ad111ff3c
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Extraction and Purification of R-Phycoerythrin Alpha Subunit from the Marine Red Algae <i>Pyropia Yezoensis</i> and Its Biological Activities

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