Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions

Abstract Magnesium is a metal used in the composition of titanium alloys and imparts porosity. Due to its osteoconductive, biocompatible and biodegradable characteristics, its application in the development of biomedical materials has become attractive. This study aimed to evaluate the influence of...

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Autores principales: Carolina da Silva Dias, Mariana Correa Rossi, Emanuel V. P. Apolonio, Gustavo dos Santos Rosa, João Pedro Hübbe Pfeifer, Carlos Alberto Hussni, Marcos Jun Watanabe, Ana Liz Garcia Alves
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Publicado: Springer 2021
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spelling oai:doaj.org-article:cfaa649e4b644e75b90cd77c099e2ba62021-12-05T12:19:16ZLow Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions10.1007/s10856-021-06620-90957-45301573-4838https://doaj.org/article/cfaa649e4b644e75b90cd77c099e2ba62021-12-01T00:00:00Zhttps://doi.org/10.1007/s10856-021-06620-9https://doaj.org/toc/0957-4530https://doaj.org/toc/1573-4838Abstract Magnesium is a metal used in the composition of titanium alloys and imparts porosity. Due to its osteoconductive, biocompatible and biodegradable characteristics, its application in the development of biomedical materials has become attractive. This study aimed to evaluate the influence of magnesium present in porous Ti-Nb-Sn alloys, which have a low elastic modulus in adhesive, osteogenic properties and the amount of reactive intracellular oxygen species released in mesenchymal stem cells derived from bone marrow equine bone (eBMMSCs). Mechanical properties of the alloy, such as hardness, compressive strength and elastic modulus, were analyzed, as well as surface morphological characteristics through scanning electron microscopy. The evaluation of magnesium ion release was performed by atomic force spectroscopy. The biological characteristics of the alloy, when in contact with the alloy surface and with the culture medium conditioned with the alloy, were studied by SEM and optical microscopy. Confirmation of osteogenic differentiation by alizarin red and detection of ROS using a Muse® Oxidative Stress Kit based on dihydroetide (DHE). The alloy showed an elastic modulus close to cortical bone values. The hardness was close to commercial Ti grade 2, and the compressive strength was greater than the value of cortical bone. The eBMMSCs adhered to the surface of the alloy during the experimental time. Osteogenic differentiation was observed with the treatment of eBMMMSCs with conditioned medium. The eBMMSCs treated with conditioned medium decreased ROS production, indicating a possible antioxidant defense potential of magnesium release.Carolina da Silva DiasMariana Correa RossiEmanuel V. P. ApolonioGustavo dos Santos RosaJoão Pedro Hübbe PfeiferCarlos Alberto HussniMarcos Jun WatanabeAna Liz Garcia AlvesSpringerarticleMaterials of engineering and construction. Mechanics of materialsTA401-492Medical technologyR855-855.5ENJournal of Materials Science: Materials in Medicine, Vol 32, Iss 12, Pp 1-11 (2021)
institution DOAJ
collection DOAJ
language EN
topic Materials of engineering and construction. Mechanics of materials
TA401-492
Medical technology
R855-855.5
spellingShingle Materials of engineering and construction. Mechanics of materials
TA401-492
Medical technology
R855-855.5
Carolina da Silva Dias
Mariana Correa Rossi
Emanuel V. P. Apolonio
Gustavo dos Santos Rosa
João Pedro Hübbe Pfeifer
Carlos Alberto Hussni
Marcos Jun Watanabe
Ana Liz Garcia Alves
Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions
description Abstract Magnesium is a metal used in the composition of titanium alloys and imparts porosity. Due to its osteoconductive, biocompatible and biodegradable characteristics, its application in the development of biomedical materials has become attractive. This study aimed to evaluate the influence of magnesium present in porous Ti-Nb-Sn alloys, which have a low elastic modulus in adhesive, osteogenic properties and the amount of reactive intracellular oxygen species released in mesenchymal stem cells derived from bone marrow equine bone (eBMMSCs). Mechanical properties of the alloy, such as hardness, compressive strength and elastic modulus, were analyzed, as well as surface morphological characteristics through scanning electron microscopy. The evaluation of magnesium ion release was performed by atomic force spectroscopy. The biological characteristics of the alloy, when in contact with the alloy surface and with the culture medium conditioned with the alloy, were studied by SEM and optical microscopy. Confirmation of osteogenic differentiation by alizarin red and detection of ROS using a Muse® Oxidative Stress Kit based on dihydroetide (DHE). The alloy showed an elastic modulus close to cortical bone values. The hardness was close to commercial Ti grade 2, and the compressive strength was greater than the value of cortical bone. The eBMMSCs adhered to the surface of the alloy during the experimental time. Osteogenic differentiation was observed with the treatment of eBMMMSCs with conditioned medium. The eBMMSCs treated with conditioned medium decreased ROS production, indicating a possible antioxidant defense potential of magnesium release.
format article
author Carolina da Silva Dias
Mariana Correa Rossi
Emanuel V. P. Apolonio
Gustavo dos Santos Rosa
João Pedro Hübbe Pfeifer
Carlos Alberto Hussni
Marcos Jun Watanabe
Ana Liz Garcia Alves
author_facet Carolina da Silva Dias
Mariana Correa Rossi
Emanuel V. P. Apolonio
Gustavo dos Santos Rosa
João Pedro Hübbe Pfeifer
Carlos Alberto Hussni
Marcos Jun Watanabe
Ana Liz Garcia Alves
author_sort Carolina da Silva Dias
title Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions
title_short Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions
title_full Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions
title_fullStr Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions
title_full_unstemmed Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions
title_sort low mg content on ti-nb-sn alloy when in contact with ebmmscs promotes improvement of its biological functions
publisher Springer
publishDate 2021
url https://doaj.org/article/cfaa649e4b644e75b90cd77c099e2ba6
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