Probiotic Lactobacillus fermentum strain JDFM216 stimulates the longevity and immune response of Caenorhabditis elegans through a nuclear hormone receptor

Abstract Here, we examined the functionality of Lactobacillus fermentum strain JDFM216, a newly isolated probiotic bacterium, using a Caenorhabditis elegans model. We determined bacterial colonization in the intestinal tract of C. elegans by plate counting and transmission electron microscopy and ex...

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Autores principales:	Mi Ri Park, Sangdon Ryu, Brighton E. Maburutse, Nam Su Oh, Sae Hun Kim, Sejong Oh, Seong-Yeop Jeong, Do-Youn Jeong, Sangnam Oh, Younghoon Kim
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cfb3e7c1885d43c1ba8d200bb991b424
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spelling	oai:doaj.org-article:cfb3e7c1885d43c1ba8d200bb991b4242021-12-02T12:32:48ZProbiotic Lactobacillus fermentum strain JDFM216 stimulates the longevity and immune response of Caenorhabditis elegans through a nuclear hormone receptor10.1038/s41598-018-25333-82045-2322https://doaj.org/article/cfb3e7c1885d43c1ba8d200bb991b4242018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25333-8https://doaj.org/toc/2045-2322Abstract Here, we examined the functionality of Lactobacillus fermentum strain JDFM216, a newly isolated probiotic bacterium, using a Caenorhabditis elegans model. We determined bacterial colonization in the intestinal tract of C. elegans by plate counting and transmission electron microscopy and examined the survival of C. elegans using a solid killing assay. In addition, we employed DNA microarray analysis, quantitative real time-polymerase chain reaction, and immunoblotting assays to explore health-promoting pathways induced by probiotic bacteria in C. elegans. Initially, we found that the probiotic bacterium L. fermentum strain JDFM216 was not harmful to the C. elegans host. Conditioning with JDFM216 led to its colonization in the nematode intestine and enhanced resistance in nematodes exposed to food-borne pathogens, including Staphylococcus aureus and Escherichia coli O157:H7. Interestingly, this probiotic strain significantly prolonged the life span of C. elegans. Whole-transcriptome analysis and transgenic worm assays revealed that the health-promoting effects of JDFM216 were mediated by a nuclear hormone receptor (NHR) family and PMK-1 signaling. Taken together, we described a new C. elegans-based system to screen novel probiotic activity and demonstrated that preconditioning with the probiotic L. fermentum strain JDFM216 may positively stimulate the longevity of the C. elegans host via specific pathway.Mi Ri ParkSangdon RyuBrighton E. MaburutseNam Su OhSae Hun KimSejong OhSeong-Yeop JeongDo-Youn JeongSangnam OhYounghoon KimNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-10 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Mi Ri Park Sangdon Ryu Brighton E. Maburutse Nam Su Oh Sae Hun Kim Sejong Oh Seong-Yeop Jeong Do-Youn Jeong Sangnam Oh Younghoon Kim Probiotic Lactobacillus fermentum strain JDFM216 stimulates the longevity and immune response of Caenorhabditis elegans through a nuclear hormone receptor
description	Abstract Here, we examined the functionality of Lactobacillus fermentum strain JDFM216, a newly isolated probiotic bacterium, using a Caenorhabditis elegans model. We determined bacterial colonization in the intestinal tract of C. elegans by plate counting and transmission electron microscopy and examined the survival of C. elegans using a solid killing assay. In addition, we employed DNA microarray analysis, quantitative real time-polymerase chain reaction, and immunoblotting assays to explore health-promoting pathways induced by probiotic bacteria in C. elegans. Initially, we found that the probiotic bacterium L. fermentum strain JDFM216 was not harmful to the C. elegans host. Conditioning with JDFM216 led to its colonization in the nematode intestine and enhanced resistance in nematodes exposed to food-borne pathogens, including Staphylococcus aureus and Escherichia coli O157:H7. Interestingly, this probiotic strain significantly prolonged the life span of C. elegans. Whole-transcriptome analysis and transgenic worm assays revealed that the health-promoting effects of JDFM216 were mediated by a nuclear hormone receptor (NHR) family and PMK-1 signaling. Taken together, we described a new C. elegans-based system to screen novel probiotic activity and demonstrated that preconditioning with the probiotic L. fermentum strain JDFM216 may positively stimulate the longevity of the C. elegans host via specific pathway.
format	article
author	Mi Ri Park Sangdon Ryu Brighton E. Maburutse Nam Su Oh Sae Hun Kim Sejong Oh Seong-Yeop Jeong Do-Youn Jeong Sangnam Oh Younghoon Kim
author_facet	Mi Ri Park Sangdon Ryu Brighton E. Maburutse Nam Su Oh Sae Hun Kim Sejong Oh Seong-Yeop Jeong Do-Youn Jeong Sangnam Oh Younghoon Kim
author_sort	Mi Ri Park
title	Probiotic Lactobacillus fermentum strain JDFM216 stimulates the longevity and immune response of Caenorhabditis elegans through a nuclear hormone receptor
title_short	Probiotic Lactobacillus fermentum strain JDFM216 stimulates the longevity and immune response of Caenorhabditis elegans through a nuclear hormone receptor
title_full	Probiotic Lactobacillus fermentum strain JDFM216 stimulates the longevity and immune response of Caenorhabditis elegans through a nuclear hormone receptor
title_fullStr	Probiotic Lactobacillus fermentum strain JDFM216 stimulates the longevity and immune response of Caenorhabditis elegans through a nuclear hormone receptor
title_full_unstemmed	Probiotic Lactobacillus fermentum strain JDFM216 stimulates the longevity and immune response of Caenorhabditis elegans through a nuclear hormone receptor
title_sort	probiotic lactobacillus fermentum strain jdfm216 stimulates the longevity and immune response of caenorhabditis elegans through a nuclear hormone receptor
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/cfb3e7c1885d43c1ba8d200bb991b424
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Probiotic Lactobacillus fermentum strain JDFM216 stimulates the longevity and immune response of Caenorhabditis elegans through a nuclear hormone receptor

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