Molecular Characterization of a Tetraspanin TSP11 Gene in Echinococcus granulosus and Evaluation Its Immunoprotection in Model Dogs

Cystic echinococcosis (CE) is a cosmopolitan zoonosis caused by the larval stage of Echinococcus granulosus, which affects humans and a wide range of mammalian intermediate hosts. Parasite tetraspanin proteins are crucial for host-parasite interactions, and therefore they may be useful for vaccine d...

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Autores principales:	Jinwen Xian, Pengpeng Zhao, Ning Wang, Weiye Wang, Yanyan Zhang, Jimeng Meng, Xun Ma, Zhengrong Wang, Xinwen Bo
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Echinococcus granulosus cystic echinococcosis Eg-TSP11 immunogenicity dog vaccine Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/cfb4f68183b445cfa2d4d8580bdc6202
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spelling	oai:doaj.org-article:cfb4f68183b445cfa2d4d8580bdc62022021-11-17T05:37:28ZMolecular Characterization of a Tetraspanin TSP11 Gene in Echinococcus granulosus and Evaluation Its Immunoprotection in Model Dogs2297-176910.3389/fvets.2021.759283https://doaj.org/article/cfb4f68183b445cfa2d4d8580bdc62022021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.759283/fullhttps://doaj.org/toc/2297-1769Cystic echinococcosis (CE) is a cosmopolitan zoonosis caused by the larval stage of Echinococcus granulosus, which affects humans and a wide range of mammalian intermediate hosts. Parasite tetraspanin proteins are crucial for host-parasite interactions, and therefore they may be useful for vaccine development or disease diagnosis. In the present study, the major antigen coding sequence of tetraspanin 11 (Eg-TSP11) from E. granulosus was determined. The results of immunolocalization showed that Eg-TSP11 was mainly located in the tegument of adult worms and protoscoleces. Western blotting analysis showed that the serum from dogs injected with recombinant Eg-TSP11 (rEg-TSP11) could recognize Eg-TSP11 among natural protoscolex proteins. Moreover, the serum from dogs with E. granulosus infection also recognized rEg-TSP11. Serum indirect enzyme-linked immunosorbent assays demonstrated that IgG levels gradually increased after the first immunization with rEg-TSP11 compared with those in the control group. Furthermore, the serum levels of interleukin 4, interleukin 5, and interferon gamma were significantly altered in the rEg-TSP11 group. Importantly, we found that vaccination with rEg-TSP11 significantly decreased worm burden and inhibited segment development in a dog model of E. granulosus infection. Based on these findings, we speculated that rEg-TSP11 might be a potential candidate vaccine antigen against E. granulosus infection in dogs.Jinwen XianJinwen XianPengpeng ZhaoPengpeng ZhaoNing WangNing WangWeiye WangWeiye WangYanyan ZhangJimeng MengXun MaZhengrong WangXinwen BoFrontiers Media S.A.articleEchinococcus granulosuscystic echinococcosisEg-TSP11immunogenicitydog vaccineVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Echinococcus granulosus cystic echinococcosis Eg-TSP11 immunogenicity dog vaccine Veterinary medicine SF600-1100
spellingShingle	Echinococcus granulosus cystic echinococcosis Eg-TSP11 immunogenicity dog vaccine Veterinary medicine SF600-1100 Jinwen Xian Jinwen Xian Pengpeng Zhao Pengpeng Zhao Ning Wang Ning Wang Weiye Wang Weiye Wang Yanyan Zhang Jimeng Meng Xun Ma Zhengrong Wang Xinwen Bo Molecular Characterization of a Tetraspanin TSP11 Gene in Echinococcus granulosus and Evaluation Its Immunoprotection in Model Dogs
description	Cystic echinococcosis (CE) is a cosmopolitan zoonosis caused by the larval stage of Echinococcus granulosus, which affects humans and a wide range of mammalian intermediate hosts. Parasite tetraspanin proteins are crucial for host-parasite interactions, and therefore they may be useful for vaccine development or disease diagnosis. In the present study, the major antigen coding sequence of tetraspanin 11 (Eg-TSP11) from E. granulosus was determined. The results of immunolocalization showed that Eg-TSP11 was mainly located in the tegument of adult worms and protoscoleces. Western blotting analysis showed that the serum from dogs injected with recombinant Eg-TSP11 (rEg-TSP11) could recognize Eg-TSP11 among natural protoscolex proteins. Moreover, the serum from dogs with E. granulosus infection also recognized rEg-TSP11. Serum indirect enzyme-linked immunosorbent assays demonstrated that IgG levels gradually increased after the first immunization with rEg-TSP11 compared with those in the control group. Furthermore, the serum levels of interleukin 4, interleukin 5, and interferon gamma were significantly altered in the rEg-TSP11 group. Importantly, we found that vaccination with rEg-TSP11 significantly decreased worm burden and inhibited segment development in a dog model of E. granulosus infection. Based on these findings, we speculated that rEg-TSP11 might be a potential candidate vaccine antigen against E. granulosus infection in dogs.
format	article
author	Jinwen Xian Jinwen Xian Pengpeng Zhao Pengpeng Zhao Ning Wang Ning Wang Weiye Wang Weiye Wang Yanyan Zhang Jimeng Meng Xun Ma Zhengrong Wang Xinwen Bo
author_facet	Jinwen Xian Jinwen Xian Pengpeng Zhao Pengpeng Zhao Ning Wang Ning Wang Weiye Wang Weiye Wang Yanyan Zhang Jimeng Meng Xun Ma Zhengrong Wang Xinwen Bo
author_sort	Jinwen Xian
title	Molecular Characterization of a Tetraspanin TSP11 Gene in Echinococcus granulosus and Evaluation Its Immunoprotection in Model Dogs
title_short	Molecular Characterization of a Tetraspanin TSP11 Gene in Echinococcus granulosus and Evaluation Its Immunoprotection in Model Dogs
title_full	Molecular Characterization of a Tetraspanin TSP11 Gene in Echinococcus granulosus and Evaluation Its Immunoprotection in Model Dogs
title_fullStr	Molecular Characterization of a Tetraspanin TSP11 Gene in Echinococcus granulosus and Evaluation Its Immunoprotection in Model Dogs
title_full_unstemmed	Molecular Characterization of a Tetraspanin TSP11 Gene in Echinococcus granulosus and Evaluation Its Immunoprotection in Model Dogs
title_sort	molecular characterization of a tetraspanin tsp11 gene in echinococcus granulosus and evaluation its immunoprotection in model dogs
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/cfb4f68183b445cfa2d4d8580bdc6202
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Molecular Characterization of a Tetraspanin TSP11 Gene in Echinococcus granulosus and Evaluation Its Immunoprotection in Model Dogs

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