An effective molecular approach for assessing cereal aphid-parasitoid-endosymbiont networks

Abstract Molecular approaches are increasingly being used to analyse host-parasitoid food webs as they overcome several hurdles inherent to conventional approaches. However, such studies have focused primarily on the detection and identification of aphids and their aphidiid primary parasitoids, larg...

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Autores principales:	Zhengpei Ye, Ines M. G. Vollhardt, Susanne Girtler, Corinna Wallinger, Zeljko Tomanovic, Michael Traugott
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cfc5d20e06d64072a21490657de5e1fa
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spelling	oai:doaj.org-article:cfc5d20e06d64072a21490657de5e1fa2021-12-02T15:04:57ZAn effective molecular approach for assessing cereal aphid-parasitoid-endosymbiont networks10.1038/s41598-017-02226-w2045-2322https://doaj.org/article/cfc5d20e06d64072a21490657de5e1fa2017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-02226-whttps://doaj.org/toc/2045-2322Abstract Molecular approaches are increasingly being used to analyse host-parasitoid food webs as they overcome several hurdles inherent to conventional approaches. However, such studies have focused primarily on the detection and identification of aphids and their aphidiid primary parasitoids, largely ignoring primary parasitoid-hyperparasitoid interactions or limiting these to a few common species within a small geographical area. Furthermore, the detection of bacterial secondary endosymbionts has not been considered in such assays despite the fact that endosymbionts may alter aphid-parasitoid interactions, as they can confer protection against parasitoids. Here we present a novel two-step multiplex PCR (MP-PCR) protocol to assess cereal aphid-primary parasitoid-hyperparasitoid-endosymbiont interactions. The first step of the assay allows detection of parasitoid DNA at a general level (24 primary and 16 hyperparasitoid species) as well as the species-specific detection of endosymbionts (3 species) and cereal aphids (3 species). The second step of the MP-PCR assay targets seven primary and six hyperparasitoid species that commonly occur in Central Europe. Additional parasitoid species not covered by the second-step of the assay can be identified via sequencing 16S rRNA amplicons generated in the first step of the assay. The approach presented here provides an efficient, highly sensitive, and cost-effective (~consumable costs of 1.3 € per sample) tool for assessing cereal aphid-parasitoid-endosymbiont interactions.Zhengpei YeInes M. G. VollhardtSusanne GirtlerCorinna WallingerZeljko TomanovicMichael TraugottNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhengpei Ye Ines M. G. Vollhardt Susanne Girtler Corinna Wallinger Zeljko Tomanovic Michael Traugott An effective molecular approach for assessing cereal aphid-parasitoid-endosymbiont networks
description	Abstract Molecular approaches are increasingly being used to analyse host-parasitoid food webs as they overcome several hurdles inherent to conventional approaches. However, such studies have focused primarily on the detection and identification of aphids and their aphidiid primary parasitoids, largely ignoring primary parasitoid-hyperparasitoid interactions or limiting these to a few common species within a small geographical area. Furthermore, the detection of bacterial secondary endosymbionts has not been considered in such assays despite the fact that endosymbionts may alter aphid-parasitoid interactions, as they can confer protection against parasitoids. Here we present a novel two-step multiplex PCR (MP-PCR) protocol to assess cereal aphid-primary parasitoid-hyperparasitoid-endosymbiont interactions. The first step of the assay allows detection of parasitoid DNA at a general level (24 primary and 16 hyperparasitoid species) as well as the species-specific detection of endosymbionts (3 species) and cereal aphids (3 species). The second step of the MP-PCR assay targets seven primary and six hyperparasitoid species that commonly occur in Central Europe. Additional parasitoid species not covered by the second-step of the assay can be identified via sequencing 16S rRNA amplicons generated in the first step of the assay. The approach presented here provides an efficient, highly sensitive, and cost-effective (~consumable costs of 1.3 € per sample) tool for assessing cereal aphid-parasitoid-endosymbiont interactions.
format	article
author	Zhengpei Ye Ines M. G. Vollhardt Susanne Girtler Corinna Wallinger Zeljko Tomanovic Michael Traugott
author_facet	Zhengpei Ye Ines M. G. Vollhardt Susanne Girtler Corinna Wallinger Zeljko Tomanovic Michael Traugott
author_sort	Zhengpei Ye
title	An effective molecular approach for assessing cereal aphid-parasitoid-endosymbiont networks
title_short	An effective molecular approach for assessing cereal aphid-parasitoid-endosymbiont networks
title_full	An effective molecular approach for assessing cereal aphid-parasitoid-endosymbiont networks
title_fullStr	An effective molecular approach for assessing cereal aphid-parasitoid-endosymbiont networks
title_full_unstemmed	An effective molecular approach for assessing cereal aphid-parasitoid-endosymbiont networks
title_sort	effective molecular approach for assessing cereal aphid-parasitoid-endosymbiont networks
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/cfc5d20e06d64072a21490657de5e1fa
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An effective molecular approach for assessing cereal aphid-parasitoid-endosymbiont networks

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