Albumin binding and anticancer effect of magnesium oxide nanoparticles

Elham Behzadi,1,* Rozhin Sarsharzadeh,1,* Mina Nouri,1 Farnoosh Attar,2 Keivan Akhtari,3 Koorosh Shahpasand,4 Mojtaba Falahati5 1Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran; 2Department of B...

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Autores principales:	Behzadi E, Sarsharzadeh R, Nouri M, Attar F, Akhtari K, Shahpasand K, Falahati M
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Spectroscopy anticancer magnesium oxide nanoparticles albumin K562 cells Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/cfcea4ef444a45b28e829829ec459653
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spelling	oai:doaj.org-article:cfcea4ef444a45b28e829829ec4596532021-12-02T02:44:29ZAlbumin binding and anticancer effect of magnesium oxide nanoparticles1178-2013https://doaj.org/article/cfcea4ef444a45b28e829829ec4596532018-12-01T00:00:00Zhttps://www.dovepress.com/albumin-binding-and-anticancer-effect-of-magnesium-oxide-nanoparticles-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Elham Behzadi,1,* Rozhin Sarsharzadeh,1,* Mina Nouri,1 Farnoosh Attar,2 Keivan Akhtari,3 Koorosh Shahpasand,4 Mojtaba Falahati5 1Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran; 2Department of Biology, Faculty of Food Industry and Agriculture, Standard Research Institute (SRI), Karaj, Iran; 3Department of Physics, University of Kurdistan, Sanandaj, Iran; 4Department of Brain and Cognitive Sciences, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran; 5Department of Nanotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran *These authors contributed equally to this work Background: Recently, nanomaterials have moved into biological and medicinal implementations like cancer therapy. Therefore, before clinical trials, their binding to plasma proteins like human serum albumin (HSA) and their cytotoxic effects against normal and cancer cell lines should be addressed. Methods: Herein, the interaction of magnesium oxide nanoparticles (MgO NPs) with HSA was studied by means of fluorescence spectroscopy, circular dichroism (CD) spectroscopy, and docking studies. Afterwards, the cytotoxic impacts of MgO NPs on human leukemia cell line (K562) and peripheral blood mononucleated cells (PBMCs) were evaluated by MTT and flow cytometry assays to quantify reactive oxygen species (ROS) generation and apoptosis. Results: It was demonstrated that MgO NPs spontaneously form a static complex with HSA molecules through hydrophobic interactions. Docking study based on the size of NPs demonstrated that different linkages can be established between MgO NPs and HSA. The CD investigation explored that MgO NPs did not alter the secondary structure of HSA. Cellular studies revealed that MgO NPs induced cytotoxicity against K562 cell lines, whereas no adverse effects were detected on PBMCs up to optimum applied concentration of MgO NPs. It was exhibited that ROS production mediated by IC50 concentrations of MgO NPs caused apoptosis-associated cell death. The pre-incubation of K562 with ROS scavenger (curcumin) inhibited the impact of MgO NPs -based apoptosis on cell fate, revealing the upstream effect of ROS in our system. Conclusion: In summary, MgO NPs may exhibit strong plasma distribution and mediate apoptosis by ROS induction in the cancer cell lines. These data demonstrate a safe aspect of MgO NPs on the proteins and normal cells and their application as a distinctive therapeutic approach in the cancer treatment. Keywords: spectroscopy, anticancer, magnesium oxide, nanoparticles, albumin, K562 cellsBehzadi ESarsharzadeh RNouri MAttar FAkhtari KShahpasand KFalahati MDove Medical PressarticleSpectroscopyanticancermagnesium oxidenanoparticlesalbuminK562 cellsMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 14, Pp 257-270 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Spectroscopy anticancer magnesium oxide nanoparticles albumin K562 cells Medicine (General) R5-920
spellingShingle	Spectroscopy anticancer magnesium oxide nanoparticles albumin K562 cells Medicine (General) R5-920 Behzadi E Sarsharzadeh R Nouri M Attar F Akhtari K Shahpasand K Falahati M Albumin binding and anticancer effect of magnesium oxide nanoparticles
description	Elham Behzadi,1,* Rozhin Sarsharzadeh,1,* Mina Nouri,1 Farnoosh Attar,2 Keivan Akhtari,3 Koorosh Shahpasand,4 Mojtaba Falahati5 1Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran; 2Department of Biology, Faculty of Food Industry and Agriculture, Standard Research Institute (SRI), Karaj, Iran; 3Department of Physics, University of Kurdistan, Sanandaj, Iran; 4Department of Brain and Cognitive Sciences, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran; 5Department of Nanotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran *These authors contributed equally to this work Background: Recently, nanomaterials have moved into biological and medicinal implementations like cancer therapy. Therefore, before clinical trials, their binding to plasma proteins like human serum albumin (HSA) and their cytotoxic effects against normal and cancer cell lines should be addressed. Methods: Herein, the interaction of magnesium oxide nanoparticles (MgO NPs) with HSA was studied by means of fluorescence spectroscopy, circular dichroism (CD) spectroscopy, and docking studies. Afterwards, the cytotoxic impacts of MgO NPs on human leukemia cell line (K562) and peripheral blood mononucleated cells (PBMCs) were evaluated by MTT and flow cytometry assays to quantify reactive oxygen species (ROS) generation and apoptosis. Results: It was demonstrated that MgO NPs spontaneously form a static complex with HSA molecules through hydrophobic interactions. Docking study based on the size of NPs demonstrated that different linkages can be established between MgO NPs and HSA. The CD investigation explored that MgO NPs did not alter the secondary structure of HSA. Cellular studies revealed that MgO NPs induced cytotoxicity against K562 cell lines, whereas no adverse effects were detected on PBMCs up to optimum applied concentration of MgO NPs. It was exhibited that ROS production mediated by IC50 concentrations of MgO NPs caused apoptosis-associated cell death. The pre-incubation of K562 with ROS scavenger (curcumin) inhibited the impact of MgO NPs -based apoptosis on cell fate, revealing the upstream effect of ROS in our system. Conclusion: In summary, MgO NPs may exhibit strong plasma distribution and mediate apoptosis by ROS induction in the cancer cell lines. These data demonstrate a safe aspect of MgO NPs on the proteins and normal cells and their application as a distinctive therapeutic approach in the cancer treatment. Keywords: spectroscopy, anticancer, magnesium oxide, nanoparticles, albumin, K562 cells
format	article
author	Behzadi E Sarsharzadeh R Nouri M Attar F Akhtari K Shahpasand K Falahati M
author_facet	Behzadi E Sarsharzadeh R Nouri M Attar F Akhtari K Shahpasand K Falahati M
author_sort	Behzadi E
title	Albumin binding and anticancer effect of magnesium oxide nanoparticles
title_short	Albumin binding and anticancer effect of magnesium oxide nanoparticles
title_full	Albumin binding and anticancer effect of magnesium oxide nanoparticles
title_fullStr	Albumin binding and anticancer effect of magnesium oxide nanoparticles
title_full_unstemmed	Albumin binding and anticancer effect of magnesium oxide nanoparticles
title_sort	albumin binding and anticancer effect of magnesium oxide nanoparticles
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/cfcea4ef444a45b28e829829ec459653
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_version_	1718402189246857216

Albumin binding and anticancer effect of magnesium oxide nanoparticles

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