Enhancing esophageal repair with bioactive bilayer mesh containing FGF

Abstract We aimed to prepare a bioactive and biodegradable bilayer mesh formed by fibroblast growth factor (FGF) loaded gelatin film layer, and poly ε-caprolactone (PCL) film layer, and to investigate its treatment efficacy on esophageal anastomosis. It is envisaged that the bioactive mesh in in viv...

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Autores principales:	Ozkan Cesur, Tugba Endogan Tanir, Pinar Celepli, Fatma Ozarslan, Sema Hucumenoglu, Adnan Karaibrahimoglu, Nesrin Hasirci
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cfcfd8a60c52425a8c40b992682f936b
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spelling	oai:doaj.org-article:cfcfd8a60c52425a8c40b992682f936b2021-12-02T17:37:40ZEnhancing esophageal repair with bioactive bilayer mesh containing FGF10.1038/s41598-021-98840-w2045-2322https://doaj.org/article/cfcfd8a60c52425a8c40b992682f936b2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98840-whttps://doaj.org/toc/2045-2322Abstract We aimed to prepare a bioactive and biodegradable bilayer mesh formed by fibroblast growth factor (FGF) loaded gelatin film layer, and poly ε-caprolactone (PCL) film layer, and to investigate its treatment efficacy on esophageal anastomosis. It is envisaged that the bioactive mesh in in vivo model would improve tissue healing in rats. The full thickness semicircular defects of 0.5 × 0.5 cm2 were created in anterior walls of abdominal esophagus. The control group had abdominal esophagus isolated with distal esophageal blunt dissection, and sham group had primary anastomosis. In the test groups, the defects were covered with bilayer polymeric meshes containing FGF (5 μg/2 cm2), or not. All rats were sacrificed for histopathology investigation after 7 or 28 days of operation. The groups are coded as FGF(−)-7th day, FGF(+)-7th day, and FGF(+)-28th day, based on their content and operation day. Highest burst pressures were obtained for FGF(+)-7th day, and FGF(+)-28th day groups (p < 0.005) and decreased inflammation grades were observed. Submucosal and muscular collagen deposition scores were markedly increased in these groups compared to sham and FGF(−)-7th day groups having no FGF (p = 0.002, p = 0.001, respectively). It was proved that FGF loaded bioactive bilayer mesh provided effective repair, reinforcement and tissue healing of esophageal defects.Ozkan CesurTugba Endogan TanirPinar CelepliFatma OzarslanSema HucumenogluAdnan KaraibrahimogluNesrin HasirciNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ozkan Cesur Tugba Endogan Tanir Pinar Celepli Fatma Ozarslan Sema Hucumenoglu Adnan Karaibrahimoglu Nesrin Hasirci Enhancing esophageal repair with bioactive bilayer mesh containing FGF
description	Abstract We aimed to prepare a bioactive and biodegradable bilayer mesh formed by fibroblast growth factor (FGF) loaded gelatin film layer, and poly ε-caprolactone (PCL) film layer, and to investigate its treatment efficacy on esophageal anastomosis. It is envisaged that the bioactive mesh in in vivo model would improve tissue healing in rats. The full thickness semicircular defects of 0.5 × 0.5 cm2 were created in anterior walls of abdominal esophagus. The control group had abdominal esophagus isolated with distal esophageal blunt dissection, and sham group had primary anastomosis. In the test groups, the defects were covered with bilayer polymeric meshes containing FGF (5 μg/2 cm2), or not. All rats were sacrificed for histopathology investigation after 7 or 28 days of operation. The groups are coded as FGF(−)-7th day, FGF(+)-7th day, and FGF(+)-28th day, based on their content and operation day. Highest burst pressures were obtained for FGF(+)-7th day, and FGF(+)-28th day groups (p < 0.005) and decreased inflammation grades were observed. Submucosal and muscular collagen deposition scores were markedly increased in these groups compared to sham and FGF(−)-7th day groups having no FGF (p = 0.002, p = 0.001, respectively). It was proved that FGF loaded bioactive bilayer mesh provided effective repair, reinforcement and tissue healing of esophageal defects.
format	article
author	Ozkan Cesur Tugba Endogan Tanir Pinar Celepli Fatma Ozarslan Sema Hucumenoglu Adnan Karaibrahimoglu Nesrin Hasirci
author_facet	Ozkan Cesur Tugba Endogan Tanir Pinar Celepli Fatma Ozarslan Sema Hucumenoglu Adnan Karaibrahimoglu Nesrin Hasirci
author_sort	Ozkan Cesur
title	Enhancing esophageal repair with bioactive bilayer mesh containing FGF
title_short	Enhancing esophageal repair with bioactive bilayer mesh containing FGF
title_full	Enhancing esophageal repair with bioactive bilayer mesh containing FGF
title_fullStr	Enhancing esophageal repair with bioactive bilayer mesh containing FGF
title_full_unstemmed	Enhancing esophageal repair with bioactive bilayer mesh containing FGF
title_sort	enhancing esophageal repair with bioactive bilayer mesh containing fgf
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cfcfd8a60c52425a8c40b992682f936b
work_keys_str_mv	AT ozkancesur enhancingesophagealrepairwithbioactivebilayermeshcontainingfgf AT tugbaendogantanir enhancingesophagealrepairwithbioactivebilayermeshcontainingfgf AT pinarcelepli enhancingesophagealrepairwithbioactivebilayermeshcontainingfgf AT fatmaozarslan enhancingesophagealrepairwithbioactivebilayermeshcontainingfgf AT semahucumenoglu enhancingesophagealrepairwithbioactivebilayermeshcontainingfgf AT adnankaraibrahimoglu enhancingesophagealrepairwithbioactivebilayermeshcontainingfgf AT nesrinhasirci enhancingesophagealrepairwithbioactivebilayermeshcontainingfgf
_version_	1718379910218645504

Enhancing esophageal repair with bioactive bilayer mesh containing FGF

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