Refinement of Active and Passive Membrane Properties of Layer V Pyramidal Neurons in Rat Primary Motor Cortex During Postnatal Development

Achieving the distinctive complex behaviors of adult mammals requires the development of a great variety of specialized neural circuits. Although the development of these circuits begins during the embryonic stage, they remain immature at birth, requiring a postnatal maturation process to achieve th...

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Autores principales:	Patricia Perez-García, Ricardo Pardillo-Díaz, Noelia Geribaldi-Doldán, Ricardo Gómez-Oliva, Samuel Domínguez-García, Carmen Castro, Pedro Nunez-Abades, Livia Carrascal
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	development motor cortex motor neurons pyramidal neurons membrane properties patch clamp Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/cfd1085ac09b4dc7b8af6077518dc26b
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spelling	oai:doaj.org-article:cfd1085ac09b4dc7b8af6077518dc26b2021-12-01T21:21:14ZRefinement of Active and Passive Membrane Properties of Layer V Pyramidal Neurons in Rat Primary Motor Cortex During Postnatal Development1662-509910.3389/fnmol.2021.754393https://doaj.org/article/cfd1085ac09b4dc7b8af6077518dc26b2021-12-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnmol.2021.754393/fullhttps://doaj.org/toc/1662-5099Achieving the distinctive complex behaviors of adult mammals requires the development of a great variety of specialized neural circuits. Although the development of these circuits begins during the embryonic stage, they remain immature at birth, requiring a postnatal maturation process to achieve these complex tasks. Understanding how the neuronal membrane properties and circuits change during development is the first step to understand their transition into efficient ones. Thus, using whole cell patch clamp recordings, we have studied the changes in the electrophysiological properties of layer V pyramidal neurons of the rat primary motor cortex during postnatal development. Among all the parameters studied, only the voltage threshold was established at birth and, although some of the changes occurred mainly during the second postnatal week, other properties such as membrane potential, capacitance, duration of the post-hyperpolarization phase or the maximum firing rate were not defined until the beginning of adulthood. Those modifications lead to a decrease in neuronal excitability and to an increase in the working range in young adult neurons, allowing more sensitive and accurate responses. This maturation process, that involves an increase in neuronal size and changes in ionic conductances, seems to be influenced by the neuronal type and by the task that neurons perform as inferred from the comparison with other pyramidal and motor neuron populations.Patricia Perez-GarcíaPatricia Perez-GarcíaRicardo Pardillo-DíazRicardo Pardillo-DíazNoelia Geribaldi-DoldánNoelia Geribaldi-DoldánRicardo Gómez-OlivaRicardo Gómez-OlivaSamuel Domínguez-GarcíaSamuel Domínguez-GarcíaCarmen CastroCarmen CastroPedro Nunez-AbadesPedro Nunez-AbadesLivia CarrascalLivia CarrascalFrontiers Media S.A.articledevelopmentmotor cortexmotor neuronspyramidal neuronsmembrane propertiespatch clampNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENFrontiers in Molecular Neuroscience, Vol 14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	development motor cortex motor neurons pyramidal neurons membrane properties patch clamp Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	development motor cortex motor neurons pyramidal neurons membrane properties patch clamp Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Patricia Perez-García Patricia Perez-García Ricardo Pardillo-Díaz Ricardo Pardillo-Díaz Noelia Geribaldi-Doldán Noelia Geribaldi-Doldán Ricardo Gómez-Oliva Ricardo Gómez-Oliva Samuel Domínguez-García Samuel Domínguez-García Carmen Castro Carmen Castro Pedro Nunez-Abades Pedro Nunez-Abades Livia Carrascal Livia Carrascal Refinement of Active and Passive Membrane Properties of Layer V Pyramidal Neurons in Rat Primary Motor Cortex During Postnatal Development
description	Achieving the distinctive complex behaviors of adult mammals requires the development of a great variety of specialized neural circuits. Although the development of these circuits begins during the embryonic stage, they remain immature at birth, requiring a postnatal maturation process to achieve these complex tasks. Understanding how the neuronal membrane properties and circuits change during development is the first step to understand their transition into efficient ones. Thus, using whole cell patch clamp recordings, we have studied the changes in the electrophysiological properties of layer V pyramidal neurons of the rat primary motor cortex during postnatal development. Among all the parameters studied, only the voltage threshold was established at birth and, although some of the changes occurred mainly during the second postnatal week, other properties such as membrane potential, capacitance, duration of the post-hyperpolarization phase or the maximum firing rate were not defined until the beginning of adulthood. Those modifications lead to a decrease in neuronal excitability and to an increase in the working range in young adult neurons, allowing more sensitive and accurate responses. This maturation process, that involves an increase in neuronal size and changes in ionic conductances, seems to be influenced by the neuronal type and by the task that neurons perform as inferred from the comparison with other pyramidal and motor neuron populations.
format	article
author	Patricia Perez-García Patricia Perez-García Ricardo Pardillo-Díaz Ricardo Pardillo-Díaz Noelia Geribaldi-Doldán Noelia Geribaldi-Doldán Ricardo Gómez-Oliva Ricardo Gómez-Oliva Samuel Domínguez-García Samuel Domínguez-García Carmen Castro Carmen Castro Pedro Nunez-Abades Pedro Nunez-Abades Livia Carrascal Livia Carrascal
author_facet	Patricia Perez-García Patricia Perez-García Ricardo Pardillo-Díaz Ricardo Pardillo-Díaz Noelia Geribaldi-Doldán Noelia Geribaldi-Doldán Ricardo Gómez-Oliva Ricardo Gómez-Oliva Samuel Domínguez-García Samuel Domínguez-García Carmen Castro Carmen Castro Pedro Nunez-Abades Pedro Nunez-Abades Livia Carrascal Livia Carrascal
author_sort	Patricia Perez-García
title	Refinement of Active and Passive Membrane Properties of Layer V Pyramidal Neurons in Rat Primary Motor Cortex During Postnatal Development
title_short	Refinement of Active and Passive Membrane Properties of Layer V Pyramidal Neurons in Rat Primary Motor Cortex During Postnatal Development
title_full	Refinement of Active and Passive Membrane Properties of Layer V Pyramidal Neurons in Rat Primary Motor Cortex During Postnatal Development
title_fullStr	Refinement of Active and Passive Membrane Properties of Layer V Pyramidal Neurons in Rat Primary Motor Cortex During Postnatal Development
title_full_unstemmed	Refinement of Active and Passive Membrane Properties of Layer V Pyramidal Neurons in Rat Primary Motor Cortex During Postnatal Development
title_sort	refinement of active and passive membrane properties of layer v pyramidal neurons in rat primary motor cortex during postnatal development
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/cfd1085ac09b4dc7b8af6077518dc26b
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Refinement of Active and Passive Membrane Properties of Layer V Pyramidal Neurons in Rat Primary Motor Cortex During Postnatal Development

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