Single-Cell Transcriptomic Analysis Revealed a Critical Role of SPP1/CD44-Mediated Crosstalk Between Macrophages and Cancer Cells in Glioma

High-grade glioma is one of the most lethal human cancers characterized by extensive tumor heterogeneity. In order to identify cellular and molecular mechanisms that drive tumor heterogeneity of this lethal disease, we performed single-cell RNA sequencing analysis of one high-grade glioma. According...

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Autores principales:	Cong He, Luoyan Sheng, Deshen Pan, Shuai Jiang, Li Ding, Xiaojun Ma, Yaohua Liu, Deshui Jia
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	glioma single-cell transcriptomic tumor heterogeneity SPP1 CD44 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/cfdd9169c15348faa87858d54e29a75c
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spelling	oai:doaj.org-article:cfdd9169c15348faa87858d54e29a75c2021-11-05T06:19:22ZSingle-Cell Transcriptomic Analysis Revealed a Critical Role of SPP1/CD44-Mediated Crosstalk Between Macrophages and Cancer Cells in Glioma2296-634X10.3389/fcell.2021.779319https://doaj.org/article/cfdd9169c15348faa87858d54e29a75c2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcell.2021.779319/fullhttps://doaj.org/toc/2296-634XHigh-grade glioma is one of the most lethal human cancers characterized by extensive tumor heterogeneity. In order to identify cellular and molecular mechanisms that drive tumor heterogeneity of this lethal disease, we performed single-cell RNA sequencing analysis of one high-grade glioma. Accordingly, we analyzed the individual cellular components in the ecosystem of this tumor. We found that tumor-associated macrophages are predominant in the immune microenvironment. Furthermore, we identified five distinct subpopulations of tumor cells, including one cycling, two OPC/NPC-like and two MES-like cell subpopulations. Moreover, we revealed the evolutionary transition from the cycling to OPC/NPC-like and MES-like cells by trajectory analysis. Importantly, we found that SPP1/CD44 interaction plays a critical role in macrophage-mediated activation of MES-like cells by exploring the cell-cell communication among all cellular components in the tumor ecosystem. Finally, we showed that high expression levels of both SPP1 and CD44 correlate with an increased infiltration of macrophages and poor prognosis of glioma patients. Taken together, this study provided a single-cell atlas of one high-grade glioma and revealed a critical role of macrophage-mediated SPP1/CD44 signaling in glioma progression, indicating that the SPP1/CD44 axis is a potential target for glioma treatment.Cong HeLuoyan ShengDeshen PanShuai JiangLi DingXiaojun MaYaohua LiuDeshui JiaFrontiers Media S.A.articlegliomasingle-cell transcriptomictumor heterogeneitySPP1CD44Biology (General)QH301-705.5ENFrontiers in Cell and Developmental Biology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	glioma single-cell transcriptomic tumor heterogeneity SPP1 CD44 Biology (General) QH301-705.5
spellingShingle	glioma single-cell transcriptomic tumor heterogeneity SPP1 CD44 Biology (General) QH301-705.5 Cong He Luoyan Sheng Deshen Pan Shuai Jiang Li Ding Xiaojun Ma Yaohua Liu Deshui Jia Single-Cell Transcriptomic Analysis Revealed a Critical Role of SPP1/CD44-Mediated Crosstalk Between Macrophages and Cancer Cells in Glioma
description	High-grade glioma is one of the most lethal human cancers characterized by extensive tumor heterogeneity. In order to identify cellular and molecular mechanisms that drive tumor heterogeneity of this lethal disease, we performed single-cell RNA sequencing analysis of one high-grade glioma. Accordingly, we analyzed the individual cellular components in the ecosystem of this tumor. We found that tumor-associated macrophages are predominant in the immune microenvironment. Furthermore, we identified five distinct subpopulations of tumor cells, including one cycling, two OPC/NPC-like and two MES-like cell subpopulations. Moreover, we revealed the evolutionary transition from the cycling to OPC/NPC-like and MES-like cells by trajectory analysis. Importantly, we found that SPP1/CD44 interaction plays a critical role in macrophage-mediated activation of MES-like cells by exploring the cell-cell communication among all cellular components in the tumor ecosystem. Finally, we showed that high expression levels of both SPP1 and CD44 correlate with an increased infiltration of macrophages and poor prognosis of glioma patients. Taken together, this study provided a single-cell atlas of one high-grade glioma and revealed a critical role of macrophage-mediated SPP1/CD44 signaling in glioma progression, indicating that the SPP1/CD44 axis is a potential target for glioma treatment.
format	article
author	Cong He Luoyan Sheng Deshen Pan Shuai Jiang Li Ding Xiaojun Ma Yaohua Liu Deshui Jia
author_facet	Cong He Luoyan Sheng Deshen Pan Shuai Jiang Li Ding Xiaojun Ma Yaohua Liu Deshui Jia
author_sort	Cong He
title	Single-Cell Transcriptomic Analysis Revealed a Critical Role of SPP1/CD44-Mediated Crosstalk Between Macrophages and Cancer Cells in Glioma
title_short	Single-Cell Transcriptomic Analysis Revealed a Critical Role of SPP1/CD44-Mediated Crosstalk Between Macrophages and Cancer Cells in Glioma
title_full	Single-Cell Transcriptomic Analysis Revealed a Critical Role of SPP1/CD44-Mediated Crosstalk Between Macrophages and Cancer Cells in Glioma
title_fullStr	Single-Cell Transcriptomic Analysis Revealed a Critical Role of SPP1/CD44-Mediated Crosstalk Between Macrophages and Cancer Cells in Glioma
title_full_unstemmed	Single-Cell Transcriptomic Analysis Revealed a Critical Role of SPP1/CD44-Mediated Crosstalk Between Macrophages and Cancer Cells in Glioma
title_sort	single-cell transcriptomic analysis revealed a critical role of spp1/cd44-mediated crosstalk between macrophages and cancer cells in glioma
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/cfdd9169c15348faa87858d54e29a75c
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Single-Cell Transcriptomic Analysis Revealed a Critical Role of SPP1/CD44-Mediated Crosstalk Between Macrophages and Cancer Cells in Glioma

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