Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota

Oplopanax elatus (Nakai) Nakai, in the Araliaceae family, has been used in traditional Chinese medicine (TCM) to treat diseases as an adaptogen for thousands of years. This study established an ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (U...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jin-Yi Wan, Jing-Xuan Wan, Shilei Wang, Xiaolu Wang, Wenqian Guo, Han Ma, Yuqi Wu, Chong-Zhi Wang, Lian-Wen Qi, Ping Li, Haiqiang Yao, Chun-Su Yuan
Formato:	article
Lenguaje:	EN
Publicado:	PeerJ Inc. 2021
Materias:	Oplopanax elatus Intestinal microbiota UPLC-Q-TOF/MS Metabolic profiles Biotransformation Medicine R
Acceso en línea:	https://doaj.org/article/cfe184d7b04248d191b44f8739578246
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cfe184d7b04248d191b44f8739578246
record_format	dspace
spelling	oai:doaj.org-article:cfe184d7b04248d191b44f87395782462021-11-26T15:05:43ZChemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota10.7717/peerj.125132167-8359https://doaj.org/article/cfe184d7b04248d191b44f87395782462021-11-01T00:00:00Zhttps://peerj.com/articles/12513.pdfhttps://peerj.com/articles/12513/https://doaj.org/toc/2167-8359Oplopanax elatus (Nakai) Nakai, in the Araliaceae family, has been used in traditional Chinese medicine (TCM) to treat diseases as an adaptogen for thousands of years. This study established an ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF/MS) method to identify chemical components and biotransformation metabolites of root bark extract from O. elatus. A total of 18 compounds were characterized in O. elatus extract, and 62 metabolites by human intestinal microbiota were detected. Two polyynes, falcarindiol and oplopandiol were recognized as the main components of O. elatus, whose metabolites are further illustrated. Several metabolic pathways were proposed to generate the detected metabolites, including methylation, hydrogenation, demethylation, dehydroxylation, and hydroxylation. These findings indicated that intestinal microbiota might play an essential role in mediating the bioactivity of O. elatus.Jin-Yi WanJing-Xuan WanShilei WangXiaolu WangWenqian GuoHan MaYuqi WuChong-Zhi WangLian-Wen QiPing LiHaiqiang YaoChun-Su YuanPeerJ Inc.articleOplopanax elatusIntestinal microbiotaUPLC-Q-TOF/MSMetabolic profilesBiotransformationMedicineRENPeerJ, Vol 9, p e12513 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Oplopanax elatus Intestinal microbiota UPLC-Q-TOF/MS Metabolic profiles Biotransformation Medicine R
spellingShingle	Oplopanax elatus Intestinal microbiota UPLC-Q-TOF/MS Metabolic profiles Biotransformation Medicine R Jin-Yi Wan Jing-Xuan Wan Shilei Wang Xiaolu Wang Wenqian Guo Han Ma Yuqi Wu Chong-Zhi Wang Lian-Wen Qi Ping Li Haiqiang Yao Chun-Su Yuan Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota
description	Oplopanax elatus (Nakai) Nakai, in the Araliaceae family, has been used in traditional Chinese medicine (TCM) to treat diseases as an adaptogen for thousands of years. This study established an ultra-performance liquid chromatography coupled with quadrupole time-of-flight tandem mass spectrometry (UPLC-Q-TOF/MS) method to identify chemical components and biotransformation metabolites of root bark extract from O. elatus. A total of 18 compounds were characterized in O. elatus extract, and 62 metabolites by human intestinal microbiota were detected. Two polyynes, falcarindiol and oplopandiol were recognized as the main components of O. elatus, whose metabolites are further illustrated. Several metabolic pathways were proposed to generate the detected metabolites, including methylation, hydrogenation, demethylation, dehydroxylation, and hydroxylation. These findings indicated that intestinal microbiota might play an essential role in mediating the bioactivity of O. elatus.
format	article
author	Jin-Yi Wan Jing-Xuan Wan Shilei Wang Xiaolu Wang Wenqian Guo Han Ma Yuqi Wu Chong-Zhi Wang Lian-Wen Qi Ping Li Haiqiang Yao Chun-Su Yuan
author_facet	Jin-Yi Wan Jing-Xuan Wan Shilei Wang Xiaolu Wang Wenqian Guo Han Ma Yuqi Wu Chong-Zhi Wang Lian-Wen Qi Ping Li Haiqiang Yao Chun-Su Yuan
author_sort	Jin-Yi Wan
title	Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota
title_short	Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota
title_full	Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota
title_fullStr	Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota
title_full_unstemmed	Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota
title_sort	chemical profiling of root bark extract from oplopanax elatus and its in vitro biotransformation by human intestinal microbiota
publisher	PeerJ Inc.
publishDate	2021
url	https://doaj.org/article/cfe184d7b04248d191b44f8739578246
work_keys_str_mv	AT jinyiwan chemicalprofilingofrootbarkextractfromoplopanaxelatusanditsinvitrobiotransformationbyhumanintestinalmicrobiota AT jingxuanwan chemicalprofilingofrootbarkextractfromoplopanaxelatusanditsinvitrobiotransformationbyhumanintestinalmicrobiota AT shileiwang chemicalprofilingofrootbarkextractfromoplopanaxelatusanditsinvitrobiotransformationbyhumanintestinalmicrobiota AT xiaoluwang chemicalprofilingofrootbarkextractfromoplopanaxelatusanditsinvitrobiotransformationbyhumanintestinalmicrobiota AT wenqianguo chemicalprofilingofrootbarkextractfromoplopanaxelatusanditsinvitrobiotransformationbyhumanintestinalmicrobiota AT hanma chemicalprofilingofrootbarkextractfromoplopanaxelatusanditsinvitrobiotransformationbyhumanintestinalmicrobiota AT yuqiwu chemicalprofilingofrootbarkextractfromoplopanaxelatusanditsinvitrobiotransformationbyhumanintestinalmicrobiota AT chongzhiwang chemicalprofilingofrootbarkextractfromoplopanaxelatusanditsinvitrobiotransformationbyhumanintestinalmicrobiota AT lianwenqi chemicalprofilingofrootbarkextractfromoplopanaxelatusanditsinvitrobiotransformationbyhumanintestinalmicrobiota AT pingli chemicalprofilingofrootbarkextractfromoplopanaxelatusanditsinvitrobiotransformationbyhumanintestinalmicrobiota AT haiqiangyao chemicalprofilingofrootbarkextractfromoplopanaxelatusanditsinvitrobiotransformationbyhumanintestinalmicrobiota AT chunsuyuan chemicalprofilingofrootbarkextractfromoplopanaxelatusanditsinvitrobiotransformationbyhumanintestinalmicrobiota
_version_	1718409318415466496

Chemical profiling of root bark extract from Oplopanax elatus and its in vitro biotransformation by human intestinal microbiota

Ejemplares similares