In-depth quantitative proteomic characterization of organotypic hippocampal slice culture reveals sex-specific differences in biochemical pathways

Abstract Sex differences in the brain of mammals range from neuroarchitecture through cognition to cellular metabolism. The hippocampus, a structure mostly associated with learning and memory, presents high vulnerability to neurodegeneration and aging. Therefore, we explored basal sex-related differ...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Simone Nardin Weis, Jaques Miranda F. Souza, Juliana Bender Hoppe, Marina Firmino, Manfred Auer, Nassim N. Ataii, Leonardo Assis da Silva, Mariana Maier Gaelzer, Caroline Peres Klein, Alan R. Mól, Consuelo M. R. de Lima, Diogo Onofre Souza, Christianne G. Salbego, Carlos André O. Ricart, Wagner Fontes, Marcelo Valle de Sousa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/cfe2a9cf46204172b40da2ef677cf9aa
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:cfe2a9cf46204172b40da2ef677cf9aa
record_format	dspace
spelling	oai:doaj.org-article:cfe2a9cf46204172b40da2ef677cf9aa2021-12-02T10:48:13ZIn-depth quantitative proteomic characterization of organotypic hippocampal slice culture reveals sex-specific differences in biochemical pathways10.1038/s41598-021-82016-72045-2322https://doaj.org/article/cfe2a9cf46204172b40da2ef677cf9aa2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82016-7https://doaj.org/toc/2045-2322Abstract Sex differences in the brain of mammals range from neuroarchitecture through cognition to cellular metabolism. The hippocampus, a structure mostly associated with learning and memory, presents high vulnerability to neurodegeneration and aging. Therefore, we explored basal sex-related differences in the proteome of organotypic hippocampal slice culture, a major in vitro model for studying the cellular and molecular mechanisms related to neurodegenerative disorders. Results suggest a greater prevalence of astrocytic metabolism in females and significant neuronal metabolism in males. The preference for glucose use in glycolysis, pentose phosphate pathway and glycogen metabolism in females and high abundance of mitochondrial respiration subunits in males support this idea. An overall upregulation of lipid metabolism was observed in females. Upregulation of proteins responsible for neuronal glutamate and GABA synthesis, along with synaptic associated proteins, were observed in males. In general, the significant spectrum of pathways known to predominate in neurons or astrocytes, together with the well-known neuronal and glial markers observed, revealed sex-specific metabolic differences in the hippocampus. TEM qualitative analysis might indicate a greater presence of mitochondria at CA1 synapses in females. These findings are crucial to a better understanding of how sex chromosomes can influence the physiology of cultured hippocampal slices and allow us to gain insights into distinct responses of males and females on neurological diseases that present a sex-biased incidence.Simone Nardin WeisJaques Miranda F. SouzaJuliana Bender HoppeMarina FirminoManfred AuerNassim N. AtaiiLeonardo Assis da SilvaMariana Maier GaelzerCaroline Peres KleinAlan R. MólConsuelo M. R. de LimaDiogo Onofre SouzaChristianne G. SalbegoCarlos André O. RicartWagner FontesMarcelo Valle de SousaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Simone Nardin Weis Jaques Miranda F. Souza Juliana Bender Hoppe Marina Firmino Manfred Auer Nassim N. Ataii Leonardo Assis da Silva Mariana Maier Gaelzer Caroline Peres Klein Alan R. Mól Consuelo M. R. de Lima Diogo Onofre Souza Christianne G. Salbego Carlos André O. Ricart Wagner Fontes Marcelo Valle de Sousa In-depth quantitative proteomic characterization of organotypic hippocampal slice culture reveals sex-specific differences in biochemical pathways
description	Abstract Sex differences in the brain of mammals range from neuroarchitecture through cognition to cellular metabolism. The hippocampus, a structure mostly associated with learning and memory, presents high vulnerability to neurodegeneration and aging. Therefore, we explored basal sex-related differences in the proteome of organotypic hippocampal slice culture, a major in vitro model for studying the cellular and molecular mechanisms related to neurodegenerative disorders. Results suggest a greater prevalence of astrocytic metabolism in females and significant neuronal metabolism in males. The preference for glucose use in glycolysis, pentose phosphate pathway and glycogen metabolism in females and high abundance of mitochondrial respiration subunits in males support this idea. An overall upregulation of lipid metabolism was observed in females. Upregulation of proteins responsible for neuronal glutamate and GABA synthesis, along with synaptic associated proteins, were observed in males. In general, the significant spectrum of pathways known to predominate in neurons or astrocytes, together with the well-known neuronal and glial markers observed, revealed sex-specific metabolic differences in the hippocampus. TEM qualitative analysis might indicate a greater presence of mitochondria at CA1 synapses in females. These findings are crucial to a better understanding of how sex chromosomes can influence the physiology of cultured hippocampal slices and allow us to gain insights into distinct responses of males and females on neurological diseases that present a sex-biased incidence.
format	article
author	Simone Nardin Weis Jaques Miranda F. Souza Juliana Bender Hoppe Marina Firmino Manfred Auer Nassim N. Ataii Leonardo Assis da Silva Mariana Maier Gaelzer Caroline Peres Klein Alan R. Mól Consuelo M. R. de Lima Diogo Onofre Souza Christianne G. Salbego Carlos André O. Ricart Wagner Fontes Marcelo Valle de Sousa
author_facet	Simone Nardin Weis Jaques Miranda F. Souza Juliana Bender Hoppe Marina Firmino Manfred Auer Nassim N. Ataii Leonardo Assis da Silva Mariana Maier Gaelzer Caroline Peres Klein Alan R. Mól Consuelo M. R. de Lima Diogo Onofre Souza Christianne G. Salbego Carlos André O. Ricart Wagner Fontes Marcelo Valle de Sousa
author_sort	Simone Nardin Weis
title	In-depth quantitative proteomic characterization of organotypic hippocampal slice culture reveals sex-specific differences in biochemical pathways
title_short	In-depth quantitative proteomic characterization of organotypic hippocampal slice culture reveals sex-specific differences in biochemical pathways
title_full	In-depth quantitative proteomic characterization of organotypic hippocampal slice culture reveals sex-specific differences in biochemical pathways
title_fullStr	In-depth quantitative proteomic characterization of organotypic hippocampal slice culture reveals sex-specific differences in biochemical pathways
title_full_unstemmed	In-depth quantitative proteomic characterization of organotypic hippocampal slice culture reveals sex-specific differences in biochemical pathways
title_sort	in-depth quantitative proteomic characterization of organotypic hippocampal slice culture reveals sex-specific differences in biochemical pathways
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/cfe2a9cf46204172b40da2ef677cf9aa
work_keys_str_mv	AT simonenardinweis indepthquantitativeproteomiccharacterizationoforganotypichippocampalsliceculturerevealssexspecificdifferencesinbiochemicalpathways AT jaquesmirandafsouza indepthquantitativeproteomiccharacterizationoforganotypichippocampalsliceculturerevealssexspecificdifferencesinbiochemicalpathways AT julianabenderhoppe indepthquantitativeproteomiccharacterizationoforganotypichippocampalsliceculturerevealssexspecificdifferencesinbiochemicalpathways AT marinafirmino indepthquantitativeproteomiccharacterizationoforganotypichippocampalsliceculturerevealssexspecificdifferencesinbiochemicalpathways AT manfredauer indepthquantitativeproteomiccharacterizationoforganotypichippocampalsliceculturerevealssexspecificdifferencesinbiochemicalpathways AT nassimnataii indepthquantitativeproteomiccharacterizationoforganotypichippocampalsliceculturerevealssexspecificdifferencesinbiochemicalpathways AT leonardoassisdasilva indepthquantitativeproteomiccharacterizationoforganotypichippocampalsliceculturerevealssexspecificdifferencesinbiochemicalpathways AT marianamaiergaelzer indepthquantitativeproteomiccharacterizationoforganotypichippocampalsliceculturerevealssexspecificdifferencesinbiochemicalpathways AT carolineperesklein indepthquantitativeproteomiccharacterizationoforganotypichippocampalsliceculturerevealssexspecificdifferencesinbiochemicalpathways AT alanrmol indepthquantitativeproteomiccharacterizationoforganotypichippocampalsliceculturerevealssexspecificdifferencesinbiochemicalpathways AT consuelomrdelima indepthquantitativeproteomiccharacterizationoforganotypichippocampalsliceculturerevealssexspecificdifferencesinbiochemicalpathways AT diogoonofresouza indepthquantitativeproteomiccharacterizationoforganotypichippocampalsliceculturerevealssexspecificdifferencesinbiochemicalpathways AT christiannegsalbego indepthquantitativeproteomiccharacterizationoforganotypichippocampalsliceculturerevealssexspecificdifferencesinbiochemicalpathways AT carlosandreoricart indepthquantitativeproteomiccharacterizationoforganotypichippocampalsliceculturerevealssexspecificdifferencesinbiochemicalpathways AT wagnerfontes indepthquantitativeproteomiccharacterizationoforganotypichippocampalsliceculturerevealssexspecificdifferencesinbiochemicalpathways AT marcelovalledesousa indepthquantitativeproteomiccharacterizationoforganotypichippocampalsliceculturerevealssexspecificdifferencesinbiochemicalpathways
_version_	1718396675456761856

In-depth quantitative proteomic characterization of organotypic hippocampal slice culture reveals sex-specific differences in biochemical pathways

Ejemplares similares