Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (<i>Synodus macrops</i>)

Marine collagen is gaining vast interest because of its high biocompatibility and lack of religious and social restrictions compared with collagen from terrestrial sources. In this study, lizardfish (<i>Synodus macrops</i>) scales were used to isolate acid-soluble collagen (ASC) and peps...

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Autores principales:	Junde Chen, Guangyu Wang, Yushuang Li
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	lizardfish scale marine collagen thermal stability cell viability Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/cfe53f4ad30b4c98b81f834825d05387
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spelling	oai:doaj.org-article:cfe53f4ad30b4c98b81f834825d053872021-11-25T18:12:42ZPreparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (<i>Synodus macrops</i>)10.3390/md191105971660-3397https://doaj.org/article/cfe53f4ad30b4c98b81f834825d053872021-10-01T00:00:00Zhttps://www.mdpi.com/1660-3397/19/11/597https://doaj.org/toc/1660-3397Marine collagen is gaining vast interest because of its high biocompatibility and lack of religious and social restrictions compared with collagen from terrestrial sources. In this study, lizardfish (<i>Synodus macrops</i>) scales were used to isolate acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC). Both ASC and PSC were identified as type I collagen with intact triple-helix structures by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and spectroscopy. The ASC and PSC had high amino acids of 237 residues/1000 residues and 236 residues/1000 residues, respectively. Thus, the maximum transition temperature (T<sub>max</sub>) of ASC (43.2 °C) was higher than that of PSC (42.5 °C). Interestingly, the T<sub>max</sub> of both ASC and PSC was higher than that of rat tail collagen (39.4 °C) and calf skin collagen (35.0 °C), the terrestrial collagen. Solubility tests showed that both ASC and PSC exhibited high solubility in the acidic pH ranges. ASC was less susceptible to the “salting out” effect compared with PSC. Both collagen types were nontoxic to HaCaT and MC3T3-E1 cells, and ASC was associated with a higher cell viability than PSC. These results indicated that ASC from lizardfish scales could be an alternative to terrestrial sources of collagen, with potential for biomedical applications.Junde ChenGuangyu WangYushuang LiMDPI AGarticlelizardfish scalemarine collagenthermal stabilitycell viabilityBiology (General)QH301-705.5ENMarine Drugs, Vol 19, Iss 597, p 597 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lizardfish scale marine collagen thermal stability cell viability Biology (General) QH301-705.5
spellingShingle	lizardfish scale marine collagen thermal stability cell viability Biology (General) QH301-705.5 Junde Chen Guangyu Wang Yushuang Li Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (<i>Synodus macrops</i>)
description	Marine collagen is gaining vast interest because of its high biocompatibility and lack of religious and social restrictions compared with collagen from terrestrial sources. In this study, lizardfish (<i>Synodus macrops</i>) scales were used to isolate acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC). Both ASC and PSC were identified as type I collagen with intact triple-helix structures by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and spectroscopy. The ASC and PSC had high amino acids of 237 residues/1000 residues and 236 residues/1000 residues, respectively. Thus, the maximum transition temperature (T<sub>max</sub>) of ASC (43.2 °C) was higher than that of PSC (42.5 °C). Interestingly, the T<sub>max</sub> of both ASC and PSC was higher than that of rat tail collagen (39.4 °C) and calf skin collagen (35.0 °C), the terrestrial collagen. Solubility tests showed that both ASC and PSC exhibited high solubility in the acidic pH ranges. ASC was less susceptible to the “salting out” effect compared with PSC. Both collagen types were nontoxic to HaCaT and MC3T3-E1 cells, and ASC was associated with a higher cell viability than PSC. These results indicated that ASC from lizardfish scales could be an alternative to terrestrial sources of collagen, with potential for biomedical applications.
format	article
author	Junde Chen Guangyu Wang Yushuang Li
author_facet	Junde Chen Guangyu Wang Yushuang Li
author_sort	Junde Chen
title	Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (<i>Synodus macrops</i>)
title_short	Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (<i>Synodus macrops</i>)
title_full	Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (<i>Synodus macrops</i>)
title_fullStr	Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (<i>Synodus macrops</i>)
title_full_unstemmed	Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (<i>Synodus macrops</i>)
title_sort	preparation and characterization of thermally stable collagens from the scales of lizardfish (<i>synodus macrops</i>)
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/cfe53f4ad30b4c98b81f834825d05387
work_keys_str_mv	AT jundechen preparationandcharacterizationofthermallystablecollagensfromthescalesoflizardfishisynodusmacropsi AT guangyuwang preparationandcharacterizationofthermallystablecollagensfromthescalesoflizardfishisynodusmacropsi AT yushuangli preparationandcharacterizationofthermallystablecollagensfromthescalesoflizardfishisynodusmacropsi
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Preparation and Characterization of Thermally Stable Collagens from the Scales of Lizardfish (<i>Synodus macrops</i>)

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