Same same, but different: The response of diatoms to environmental gradients in Fennoscandian streams and lakes – barcodes, traits and microscope data compared

We developed and compared the performance of freshwater benthic diatom indices calculated from (i) traditional morphological species identification, (ii) Amplicon Sequence Variants (ASVs) obtained via DNA metabarcoding, and (iii) morphological traits to indicate eutrophication in rivers and lakes in...

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Autores principales:	Maria Kahlert, Bonnie Bailet, Teofana Chonova, Satu Maaria Karjalainen, Susanne C. Schneider, Kálmán Tapolczai
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Amplicon Sequence Variants Environmental assessment Index Indicator value Eutrophication Metabarcoding Ecology QH540-549.5
Acceso en línea:	https://doaj.org/article/cff5faff3c8c4853a93d1d0ed28abe47
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spelling	oai:doaj.org-article:cff5faff3c8c4853a93d1d0ed28abe472021-12-01T04:58:58ZSame same, but different: The response of diatoms to environmental gradients in Fennoscandian streams and lakes – barcodes, traits and microscope data compared1470-160X10.1016/j.ecolind.2021.108088https://doaj.org/article/cff5faff3c8c4853a93d1d0ed28abe472021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1470160X21007536https://doaj.org/toc/1470-160XWe developed and compared the performance of freshwater benthic diatom indices calculated from (i) traditional morphological species identification, (ii) Amplicon Sequence Variants (ASVs) obtained via DNA metabarcoding, and (iii) morphological traits to indicate eutrophication in rivers and lakes in Fennoscandia. Based on the results, we provided recommendations for the future routine use of diatom bioassessment tools in environmental monitoring and assessment. Our results show that ASVs are the most promising candidates to be used in environmental assessment. Indices based on ASVs correlated better with TotP concentrations than morphological taxa data, whereas the trait indices correlated least. We could see by studying the taxonomic assignments of the ASVs that traditional morphotaxa were divided up into several ASVs with different ecological profiles, which explained part of the better index performance and also encourages further studies on diatom diversity and ecological preferences. In general, ASV- and morphotaxon-specific optima differed slightly between streams and lakes, but were significantly correlated with each other. This means that it should be possible to develop a common index that is applicable in both streams and lakes, but boundary values with respect to TotP might need to be set separately for them. More knowledge on diatom traits is required to enable their use for environmental assessment.Maria KahlertBonnie BailetTeofana ChonovaSatu Maaria KarjalainenSusanne C. SchneiderKálmán TapolczaiElsevierarticleAmplicon Sequence VariantsEnvironmental assessmentIndexIndicator valueEutrophicationMetabarcodingEcologyQH540-549.5ENEcological Indicators, Vol 130, Iss , Pp 108088- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Amplicon Sequence Variants Environmental assessment Index Indicator value Eutrophication Metabarcoding Ecology QH540-549.5
spellingShingle	Amplicon Sequence Variants Environmental assessment Index Indicator value Eutrophication Metabarcoding Ecology QH540-549.5 Maria Kahlert Bonnie Bailet Teofana Chonova Satu Maaria Karjalainen Susanne C. Schneider Kálmán Tapolczai Same same, but different: The response of diatoms to environmental gradients in Fennoscandian streams and lakes – barcodes, traits and microscope data compared
description	We developed and compared the performance of freshwater benthic diatom indices calculated from (i) traditional morphological species identification, (ii) Amplicon Sequence Variants (ASVs) obtained via DNA metabarcoding, and (iii) morphological traits to indicate eutrophication in rivers and lakes in Fennoscandia. Based on the results, we provided recommendations for the future routine use of diatom bioassessment tools in environmental monitoring and assessment. Our results show that ASVs are the most promising candidates to be used in environmental assessment. Indices based on ASVs correlated better with TotP concentrations than morphological taxa data, whereas the trait indices correlated least. We could see by studying the taxonomic assignments of the ASVs that traditional morphotaxa were divided up into several ASVs with different ecological profiles, which explained part of the better index performance and also encourages further studies on diatom diversity and ecological preferences. In general, ASV- and morphotaxon-specific optima differed slightly between streams and lakes, but were significantly correlated with each other. This means that it should be possible to develop a common index that is applicable in both streams and lakes, but boundary values with respect to TotP might need to be set separately for them. More knowledge on diatom traits is required to enable their use for environmental assessment.
format	article
author	Maria Kahlert Bonnie Bailet Teofana Chonova Satu Maaria Karjalainen Susanne C. Schneider Kálmán Tapolczai
author_facet	Maria Kahlert Bonnie Bailet Teofana Chonova Satu Maaria Karjalainen Susanne C. Schneider Kálmán Tapolczai
author_sort	Maria Kahlert
title	Same same, but different: The response of diatoms to environmental gradients in Fennoscandian streams and lakes – barcodes, traits and microscope data compared
title_short	Same same, but different: The response of diatoms to environmental gradients in Fennoscandian streams and lakes – barcodes, traits and microscope data compared
title_full	Same same, but different: The response of diatoms to environmental gradients in Fennoscandian streams and lakes – barcodes, traits and microscope data compared
title_fullStr	Same same, but different: The response of diatoms to environmental gradients in Fennoscandian streams and lakes – barcodes, traits and microscope data compared
title_full_unstemmed	Same same, but different: The response of diatoms to environmental gradients in Fennoscandian streams and lakes – barcodes, traits and microscope data compared
title_sort	same same, but different: the response of diatoms to environmental gradients in fennoscandian streams and lakes – barcodes, traits and microscope data compared
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/cff5faff3c8c4853a93d1d0ed28abe47
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Same same, but different: The response of diatoms to environmental gradients in Fennoscandian streams and lakes – barcodes, traits and microscope data compared

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