Interaction of erythrocyte membranes with derivatives of 3-oxypiridines

In connection with the increased interest in the use of auto-erythrocytes for directional drug transport, the study of the ability of erythrocytes to sorb an antioxidant drug is becoming topical. The article considers the method of spectrophotometry developed for evaluating the ability of erythrocyt...

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Autores principales:	E. E. Kuznetsova, V. G. Gorokhova, Y. I. Pivovarov, L. A. Dmitrieva, O. V. Bogdanova
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2017
Materias:	red blood cells the spectrophotometric method mexidol Science Q
Acceso en línea:	https://doaj.org/article/d00185caf4f34023a448a094f7700a86
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spelling	oai:doaj.org-article:d00185caf4f34023a448a094f7700a862021-11-23T06:14:38ZInteraction of erythrocyte membranes with derivatives of 3-oxypiridines2541-94202587-959610.12737/article_5a3a0dbe2be5a0.22172442https://doaj.org/article/d00185caf4f34023a448a094f7700a862017-09-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/508https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596In connection with the increased interest in the use of auto-erythrocytes for directional drug transport, the study of the ability of erythrocytes to sorb an antioxidant drug is becoming topical. The article considers the method of spectrophotometry developed for evaluating the ability of erythrocytes to load with a mexidol preparation under conditions of natural sorption and a method created for determining the drug concentration in erythrocyte biological medias. Materials and methods. The peripheral blood of 15 clinically healthy males aged 20 to 45 years was used as an object of the study. A pharmaceutical preparation of oxymethylethylpyridine succinate-mexidol (ZAO «Pharmasoft», Russia) was used for clinical studies. The drug belongs to the group of 3-hydroxypyridines. The inclusion of mexidol was carried out by direct incubation of erythrocytes in a medium containing this preparation. The concentrations of the preparation were 1.25,2.5 and 5 ßg/ml and the incubation time was 15,20 and 3o minutes. The supernatant was obtained by centrifugation for 10 minutes at 3000 rpm on a SF-2000 spectrophotometer at a wavelength of 630 nm. In addition to the pharmacopoeial method, the oxidation-reduction reaction of the supernatant with methylene blue was used. Results. When measuring the spectra of the supernatant with different concentration of the preparation and using methylene blue, a regression relationship between mexidol and optical density was established, and the optimal exposure time of red blood cells and the drug was determined. These data can be used to control the directional transport of the drug to target organs. A model of the equation for determination of the mexidol content in biomedids is proposed.E. E. KuznetsovaV. G. GorokhovaY. I. PivovarovL. A. DmitrievaO. V. BogdanovaScientific Сentre for Family Health and Human Reproduction Problemsarticlered blood cellsthe spectrophotometric methodmexidolScienceQRUActa Biomedica Scientifica, Vol 2, Iss 5(2), Pp 60-64 (2017)
institution	DOAJ
collection	DOAJ
language	RU
topic	red blood cells the spectrophotometric method mexidol Science Q
spellingShingle	red blood cells the spectrophotometric method mexidol Science Q E. E. Kuznetsova V. G. Gorokhova Y. I. Pivovarov L. A. Dmitrieva O. V. Bogdanova Interaction of erythrocyte membranes with derivatives of 3-oxypiridines
description	In connection with the increased interest in the use of auto-erythrocytes for directional drug transport, the study of the ability of erythrocytes to sorb an antioxidant drug is becoming topical. The article considers the method of spectrophotometry developed for evaluating the ability of erythrocytes to load with a mexidol preparation under conditions of natural sorption and a method created for determining the drug concentration in erythrocyte biological medias. Materials and methods. The peripheral blood of 15 clinically healthy males aged 20 to 45 years was used as an object of the study. A pharmaceutical preparation of oxymethylethylpyridine succinate-mexidol (ZAO «Pharmasoft», Russia) was used for clinical studies. The drug belongs to the group of 3-hydroxypyridines. The inclusion of mexidol was carried out by direct incubation of erythrocytes in a medium containing this preparation. The concentrations of the preparation were 1.25,2.5 and 5 ßg/ml and the incubation time was 15,20 and 3o minutes. The supernatant was obtained by centrifugation for 10 minutes at 3000 rpm on a SF-2000 spectrophotometer at a wavelength of 630 nm. In addition to the pharmacopoeial method, the oxidation-reduction reaction of the supernatant with methylene blue was used. Results. When measuring the spectra of the supernatant with different concentration of the preparation and using methylene blue, a regression relationship between mexidol and optical density was established, and the optimal exposure time of red blood cells and the drug was determined. These data can be used to control the directional transport of the drug to target organs. A model of the equation for determination of the mexidol content in biomedids is proposed.
format	article
author	E. E. Kuznetsova V. G. Gorokhova Y. I. Pivovarov L. A. Dmitrieva O. V. Bogdanova
author_facet	E. E. Kuznetsova V. G. Gorokhova Y. I. Pivovarov L. A. Dmitrieva O. V. Bogdanova
author_sort	E. E. Kuznetsova
title	Interaction of erythrocyte membranes with derivatives of 3-oxypiridines
title_short	Interaction of erythrocyte membranes with derivatives of 3-oxypiridines
title_full	Interaction of erythrocyte membranes with derivatives of 3-oxypiridines
title_fullStr	Interaction of erythrocyte membranes with derivatives of 3-oxypiridines
title_full_unstemmed	Interaction of erythrocyte membranes with derivatives of 3-oxypiridines
title_sort	interaction of erythrocyte membranes with derivatives of 3-oxypiridines
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2017
url	https://doaj.org/article/d00185caf4f34023a448a094f7700a86
work_keys_str_mv	AT eekuznetsova interactionoferythrocytemembraneswithderivativesof3oxypiridines AT vggorokhova interactionoferythrocytemembraneswithderivativesof3oxypiridines AT yipivovarov interactionoferythrocytemembraneswithderivativesof3oxypiridines AT ladmitrieva interactionoferythrocytemembraneswithderivativesof3oxypiridines AT ovbogdanova interactionoferythrocytemembraneswithderivativesof3oxypiridines
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Interaction of erythrocyte membranes with derivatives of 3-oxypiridines

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