Characterization of microRNA Profiles in Pasteurella multocida-Infected Rabbits and Identification of miR-29-5p as a Regulator of Antibacterial Immune Response

Pasteurella multocida is the pathogenic agent for a variety of severe diseases in livestock, including rabbits. MicroRNAs (miRNAs) participate in the immune response to the pathogen. Distinct miRNA expression patterns were explored in rabbit lung by small-RNA deep sequencing to assess dysregulated m...

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Autores principales:	Jiaqing Hu, Wenqiang Li, Xibo Qiao, Wenjie Li, Kerui Xie, Yanyan Wang, Bing Huang, Qiaoya Zhao, Lei Liu, Xinzhong Fan
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Pasteurella multocida small RNA-seq ocu-miR-29-5p target gene antibacterial immune Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/d0111ff32e694c9d89e69ae6e707cf08
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spelling	oai:doaj.org-article:d0111ff32e694c9d89e69ae6e707cf082021-11-17T05:39:24ZCharacterization of microRNA Profiles in Pasteurella multocida-Infected Rabbits and Identification of miR-29-5p as a Regulator of Antibacterial Immune Response2297-176910.3389/fvets.2021.746638https://doaj.org/article/d0111ff32e694c9d89e69ae6e707cf082021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.746638/fullhttps://doaj.org/toc/2297-1769Pasteurella multocida is the pathogenic agent for a variety of severe diseases in livestock, including rabbits. MicroRNAs (miRNAs) participate in the immune response to the pathogen. Distinct miRNA expression patterns were explored in rabbit lung by small-RNA deep sequencing to assess dysregulated miRNAs during P. multocida infection. Totally, 571 miRNAs were screened, of which, 62 were novel, and 32 exhibited differential expression (DE). Of the 32 known DE-miRNAs, 13 and 15 occurred at 1 day and 3 days post-infection (dpi); and ocu-miR-107-3p and ocu-miR-29b-5p were shared between the two time points. Moreover, 7,345 non-redundant target genes were predicted for the 32 DE-miRNAs. Putative target genes were enriched in diverse GO and KEGG pathways and might be crucial for disease resistance. Interestingly, upregulation of ocu-miR-29-5p suppresses P. multocida propagation and downregulates expression of epithelial membrane protein-2 (EMP2) and T-box 4 (TBX4) genes by binding to their 3′ untranslated region in RK13 cells. Thus, ocu-miR-29-5p may indirectly inhibit P. multocida invasion by modulating genes related to the host immune response, such as EMP2 and TBX4.Jiaqing HuWenqiang LiXibo QiaoWenjie LiKerui XieYanyan WangBing HuangQiaoya ZhaoLei LiuXinzhong FanFrontiers Media S.A.articlePasteurella multocidasmall RNA-seqocu-miR-29-5ptarget geneantibacterial immuneVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pasteurella multocida small RNA-seq ocu-miR-29-5p target gene antibacterial immune Veterinary medicine SF600-1100
spellingShingle	Pasteurella multocida small RNA-seq ocu-miR-29-5p target gene antibacterial immune Veterinary medicine SF600-1100 Jiaqing Hu Wenqiang Li Xibo Qiao Wenjie Li Kerui Xie Yanyan Wang Bing Huang Qiaoya Zhao Lei Liu Xinzhong Fan Characterization of microRNA Profiles in Pasteurella multocida-Infected Rabbits and Identification of miR-29-5p as a Regulator of Antibacterial Immune Response
description	Pasteurella multocida is the pathogenic agent for a variety of severe diseases in livestock, including rabbits. MicroRNAs (miRNAs) participate in the immune response to the pathogen. Distinct miRNA expression patterns were explored in rabbit lung by small-RNA deep sequencing to assess dysregulated miRNAs during P. multocida infection. Totally, 571 miRNAs were screened, of which, 62 were novel, and 32 exhibited differential expression (DE). Of the 32 known DE-miRNAs, 13 and 15 occurred at 1 day and 3 days post-infection (dpi); and ocu-miR-107-3p and ocu-miR-29b-5p were shared between the two time points. Moreover, 7,345 non-redundant target genes were predicted for the 32 DE-miRNAs. Putative target genes were enriched in diverse GO and KEGG pathways and might be crucial for disease resistance. Interestingly, upregulation of ocu-miR-29-5p suppresses P. multocida propagation and downregulates expression of epithelial membrane protein-2 (EMP2) and T-box 4 (TBX4) genes by binding to their 3′ untranslated region in RK13 cells. Thus, ocu-miR-29-5p may indirectly inhibit P. multocida invasion by modulating genes related to the host immune response, such as EMP2 and TBX4.
format	article
author	Jiaqing Hu Wenqiang Li Xibo Qiao Wenjie Li Kerui Xie Yanyan Wang Bing Huang Qiaoya Zhao Lei Liu Xinzhong Fan
author_facet	Jiaqing Hu Wenqiang Li Xibo Qiao Wenjie Li Kerui Xie Yanyan Wang Bing Huang Qiaoya Zhao Lei Liu Xinzhong Fan
author_sort	Jiaqing Hu
title	Characterization of microRNA Profiles in Pasteurella multocida-Infected Rabbits and Identification of miR-29-5p as a Regulator of Antibacterial Immune Response
title_short	Characterization of microRNA Profiles in Pasteurella multocida-Infected Rabbits and Identification of miR-29-5p as a Regulator of Antibacterial Immune Response
title_full	Characterization of microRNA Profiles in Pasteurella multocida-Infected Rabbits and Identification of miR-29-5p as a Regulator of Antibacterial Immune Response
title_fullStr	Characterization of microRNA Profiles in Pasteurella multocida-Infected Rabbits and Identification of miR-29-5p as a Regulator of Antibacterial Immune Response
title_full_unstemmed	Characterization of microRNA Profiles in Pasteurella multocida-Infected Rabbits and Identification of miR-29-5p as a Regulator of Antibacterial Immune Response
title_sort	characterization of microrna profiles in pasteurella multocida-infected rabbits and identification of mir-29-5p as a regulator of antibacterial immune response
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/d0111ff32e694c9d89e69ae6e707cf08
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Characterization of microRNA Profiles in Pasteurella multocida-Infected Rabbits and Identification of miR-29-5p as a Regulator of Antibacterial Immune Response

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