Diversity of biocontrol agents, isolated from several sources, inhibitory to several fungal plant pathogens

Abstract. Ramona Y, Darmayasa IBG, Kusuma AANN, Line MA. 2021. Diversity of biocontrol agents, isolated from several sources, inhibitory to several fungal plant pathogens. Biodiversitas 22: 298-303. This study investigated the inhibitory potential of diversity of antagonist bacteria residing in the...

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Autores principales:	Yan Ramona, IDA BAGUS GEDE DARMAYASA, ANAK AGUNG NGURAH NARA KUSUMA, Martin Line
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	antagonist, biological control, compost, diversity, plant pathogens, rhizosphere Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/d0174cff80c743c984aa81b8586cc511
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spelling	oai:doaj.org-article:d0174cff80c743c984aa81b8586cc5112021-11-22T00:53:56ZDiversity of biocontrol agents, isolated from several sources, inhibitory to several fungal plant pathogens1412-033X2085-472210.13057/biodiv/d220136https://doaj.org/article/d0174cff80c743c984aa81b8586cc5112020-12-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7198https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Ramona Y, Darmayasa IBG, Kusuma AANN, Line MA. 2021. Diversity of biocontrol agents, isolated from several sources, inhibitory to several fungal plant pathogens. Biodiversitas 22: 298-303. This study investigated the inhibitory potential of diversity of antagonist bacteria residing in the rhizosphere zone and mature compost to counter fungal plant pathogens. Soils collected from rhizosphere of lettuce farms in Bali-Indonesia and Tasmania-Australia, mature compost, commercial biocontrol (Dipel®), and laboratory contaminants with significant inhibition against tested fungal pathogens were used as sources of antagonist bacteria. These antagonists were isolated by applying dilution and spread method on trypticase soya agar (TSA) or potato dextrose agar (PDA), and their ability to inhibit Sclerotinia minor, Sclerotinia sclerotiorum, Fusarium spp., and Rhizoctonia solani was assessed in dual culture assays. The results showed that 67 out of more than 100 isolates had antagonistic activity in vitro against at least one of tested fungal pathogens. In the preliminary identification, Bacillus spp. or Pseudomonas spp. were found to be pre-dominant isolates. Following screening studies in a non-replicated glasshouse experiment against S. minor and S. sclerotiorum, 8 of the most promising isolates were further identified using molecular methods based on their 16s rDNA sequences aligned with those deposited at the GeneBank. These 8 isolates were identified as Pseudomonas corrugata, Bacillus megaterium, Bacillus polymyxa, Bacillus mojavensis, Bacillus pumilus, Bacillus thuringiensis, Exiguobacterium acetylicum, and Chryseobacterium indologenes.Yan RamonaIDA BAGUS GEDE DARMAYASAANAK AGUNG NGURAH NARA KUSUMAMartin LineMBI & UNS Soloarticleantagonist, biological control, compost, diversity, plant pathogens, rhizosphereBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 1 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	antagonist, biological control, compost, diversity, plant pathogens, rhizosphere Biology (General) QH301-705.5
spellingShingle	antagonist, biological control, compost, diversity, plant pathogens, rhizosphere Biology (General) QH301-705.5 Yan Ramona IDA BAGUS GEDE DARMAYASA ANAK AGUNG NGURAH NARA KUSUMA Martin Line Diversity of biocontrol agents, isolated from several sources, inhibitory to several fungal plant pathogens
description	Abstract. Ramona Y, Darmayasa IBG, Kusuma AANN, Line MA. 2021. Diversity of biocontrol agents, isolated from several sources, inhibitory to several fungal plant pathogens. Biodiversitas 22: 298-303. This study investigated the inhibitory potential of diversity of antagonist bacteria residing in the rhizosphere zone and mature compost to counter fungal plant pathogens. Soils collected from rhizosphere of lettuce farms in Bali-Indonesia and Tasmania-Australia, mature compost, commercial biocontrol (Dipel®), and laboratory contaminants with significant inhibition against tested fungal pathogens were used as sources of antagonist bacteria. These antagonists were isolated by applying dilution and spread method on trypticase soya agar (TSA) or potato dextrose agar (PDA), and their ability to inhibit Sclerotinia minor, Sclerotinia sclerotiorum, Fusarium spp., and Rhizoctonia solani was assessed in dual culture assays. The results showed that 67 out of more than 100 isolates had antagonistic activity in vitro against at least one of tested fungal pathogens. In the preliminary identification, Bacillus spp. or Pseudomonas spp. were found to be pre-dominant isolates. Following screening studies in a non-replicated glasshouse experiment against S. minor and S. sclerotiorum, 8 of the most promising isolates were further identified using molecular methods based on their 16s rDNA sequences aligned with those deposited at the GeneBank. These 8 isolates were identified as Pseudomonas corrugata, Bacillus megaterium, Bacillus polymyxa, Bacillus mojavensis, Bacillus pumilus, Bacillus thuringiensis, Exiguobacterium acetylicum, and Chryseobacterium indologenes.
format	article
author	Yan Ramona IDA BAGUS GEDE DARMAYASA ANAK AGUNG NGURAH NARA KUSUMA Martin Line
author_facet	Yan Ramona IDA BAGUS GEDE DARMAYASA ANAK AGUNG NGURAH NARA KUSUMA Martin Line
author_sort	Yan Ramona
title	Diversity of biocontrol agents, isolated from several sources, inhibitory to several fungal plant pathogens
title_short	Diversity of biocontrol agents, isolated from several sources, inhibitory to several fungal plant pathogens
title_full	Diversity of biocontrol agents, isolated from several sources, inhibitory to several fungal plant pathogens
title_fullStr	Diversity of biocontrol agents, isolated from several sources, inhibitory to several fungal plant pathogens
title_full_unstemmed	Diversity of biocontrol agents, isolated from several sources, inhibitory to several fungal plant pathogens
title_sort	diversity of biocontrol agents, isolated from several sources, inhibitory to several fungal plant pathogens
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/d0174cff80c743c984aa81b8586cc511
work_keys_str_mv	AT yanramona diversityofbiocontrolagentsisolatedfromseveralsourcesinhibitorytoseveralfungalplantpathogens AT idabagusgededarmayasa diversityofbiocontrolagentsisolatedfromseveralsourcesinhibitorytoseveralfungalplantpathogens AT anakagungngurahnarakusuma diversityofbiocontrolagentsisolatedfromseveralsourcesinhibitorytoseveralfungalplantpathogens AT martinline diversityofbiocontrolagentsisolatedfromseveralsourcesinhibitorytoseveralfungalplantpathogens
_version_	1718418489663815680

Diversity of biocontrol agents, isolated from several sources, inhibitory to several fungal plant pathogens

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