Zingiber Officinale Var. Rubrum Extract Increases The Cytotoxic Activity Of 5-Fluorouracil In Colon Adenocarcinoma Widr Cells

One of the main chemotherapy agents for colon cancer is 5-fluorouracil (5-FU). However, the effectiveness of 5-FU decreased; therefore, co-chemotherapy with another agent is needed to enhance the activity. This study aims to determine the effect of red ginger extract as a co-chemotherapy agent with...

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Autores principales:	Sarmoko Sarmoko, Iin Solihati, Joko Setyono, Heny Ekowati, Arif Fadlan
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Gadjah Mada 2020
Materias:	red ginger, 5-fluorouracil, widr cell lines, cochemotherapy, cytotoxic effect Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/d0213fac548e45a9a5fd59ade2c3add8
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spelling	oai:doaj.org-article:d0213fac548e45a9a5fd59ade2c3add82021-11-15T06:08:47ZZingiber Officinale Var. Rubrum Extract Increases The Cytotoxic Activity Of 5-Fluorouracil In Colon Adenocarcinoma Widr Cells2338-94272338-948610.22146/ijp.859https://doaj.org/article/d0213fac548e45a9a5fd59ade2c3add82020-12-01T00:00:00Zhttps://jurnal.ugm.ac.id/v3/IJP/article/view/859https://doaj.org/toc/2338-9427https://doaj.org/toc/2338-9486One of the main chemotherapy agents for colon cancer is 5-fluorouracil (5-FU). However, the effectiveness of 5-FU decreased; therefore, co-chemotherapy with another agent is needed to enhance the activity. This study aims to determine the effect of red ginger extract as a co-chemotherapy agent with 5-FU on WiDr colon adenocarcinoma cells. We investigated the cytotoxic activity of the ethanolic extract of rhizome red ginger and combination with 5-fluorouracil (5-FU) in WiDr human colorectal cancer cell line. Red ginger extract was extracted by using 96% ethanol. Cytotoxic assay of red ginger extract and 5-FU was performed using MTT assay for 24 and 48 hours. Treatment of 5-FU in WiDr cells for 48 hours showed an IC50 value of 130 µg/mL, while no IC50 value was obtained for the 24 hours treatment. Treatment of red ginger extract with an incubation time of 24 and 48 hours had a cytotoxic effect on WiDr cells with IC50 values of 68 µg/mL and 65 µg/mL, respectively. The combination of 1000 μg/mL 5-fluorouracil with 35 μg/mL red ginger extract at 24-hour incubation resulted in a smaller cell viability value than every single treatment. The combination treatment of 5-FU 60 μg/mL with red ginger extract at a 25 μg/mL concentration causes a more significant decrease in cell viability than a single 5-FU treatment. In conclusion, red ginger extract may increase the cytotoxic activity of 5-FU in colon adenocarcinoma WiDr cells.Sarmoko SarmokoIin SolihatiJoko SetyonoHeny EkowatiArif FadlanUniversitas Gadjah Madaarticlered ginger, 5-fluorouracil, widr cell lines, cochemotherapy, cytotoxic effectPharmacy and materia medicaRS1-441ENIndonesian Journal of Pharmacy, Pp 266-272 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	red ginger, 5-fluorouracil, widr cell lines, cochemotherapy, cytotoxic effect Pharmacy and materia medica RS1-441
spellingShingle	red ginger, 5-fluorouracil, widr cell lines, cochemotherapy, cytotoxic effect Pharmacy and materia medica RS1-441 Sarmoko Sarmoko Iin Solihati Joko Setyono Heny Ekowati Arif Fadlan Zingiber Officinale Var. Rubrum Extract Increases The Cytotoxic Activity Of 5-Fluorouracil In Colon Adenocarcinoma Widr Cells
description	One of the main chemotherapy agents for colon cancer is 5-fluorouracil (5-FU). However, the effectiveness of 5-FU decreased; therefore, co-chemotherapy with another agent is needed to enhance the activity. This study aims to determine the effect of red ginger extract as a co-chemotherapy agent with 5-FU on WiDr colon adenocarcinoma cells. We investigated the cytotoxic activity of the ethanolic extract of rhizome red ginger and combination with 5-fluorouracil (5-FU) in WiDr human colorectal cancer cell line. Red ginger extract was extracted by using 96% ethanol. Cytotoxic assay of red ginger extract and 5-FU was performed using MTT assay for 24 and 48 hours. Treatment of 5-FU in WiDr cells for 48 hours showed an IC50 value of 130 µg/mL, while no IC50 value was obtained for the 24 hours treatment. Treatment of red ginger extract with an incubation time of 24 and 48 hours had a cytotoxic effect on WiDr cells with IC50 values of 68 µg/mL and 65 µg/mL, respectively. The combination of 1000 μg/mL 5-fluorouracil with 35 μg/mL red ginger extract at 24-hour incubation resulted in a smaller cell viability value than every single treatment. The combination treatment of 5-FU 60 μg/mL with red ginger extract at a 25 μg/mL concentration causes a more significant decrease in cell viability than a single 5-FU treatment. In conclusion, red ginger extract may increase the cytotoxic activity of 5-FU in colon adenocarcinoma WiDr cells.
format	article
author	Sarmoko Sarmoko Iin Solihati Joko Setyono Heny Ekowati Arif Fadlan
author_facet	Sarmoko Sarmoko Iin Solihati Joko Setyono Heny Ekowati Arif Fadlan
author_sort	Sarmoko Sarmoko
title	Zingiber Officinale Var. Rubrum Extract Increases The Cytotoxic Activity Of 5-Fluorouracil In Colon Adenocarcinoma Widr Cells
title_short	Zingiber Officinale Var. Rubrum Extract Increases The Cytotoxic Activity Of 5-Fluorouracil In Colon Adenocarcinoma Widr Cells
title_full	Zingiber Officinale Var. Rubrum Extract Increases The Cytotoxic Activity Of 5-Fluorouracil In Colon Adenocarcinoma Widr Cells
title_fullStr	Zingiber Officinale Var. Rubrum Extract Increases The Cytotoxic Activity Of 5-Fluorouracil In Colon Adenocarcinoma Widr Cells
title_full_unstemmed	Zingiber Officinale Var. Rubrum Extract Increases The Cytotoxic Activity Of 5-Fluorouracil In Colon Adenocarcinoma Widr Cells
title_sort	zingiber officinale var. rubrum extract increases the cytotoxic activity of 5-fluorouracil in colon adenocarcinoma widr cells
publisher	Universitas Gadjah Mada
publishDate	2020
url	https://doaj.org/article/d0213fac548e45a9a5fd59ade2c3add8
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Zingiber Officinale Var. Rubrum Extract Increases The Cytotoxic Activity Of 5-Fluorouracil In Colon Adenocarcinoma Widr Cells

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