Size Dependent Uptake and Hemolytic Effect of Zinc Oxide Nanoparticles on Erythrocytes and Biomedical Potential of ZnO-Ferulic acid Conjugates

Abstract Despite zinc oxide nanoparticles (ZnONPs) being increasingly used as carriers in biomedical fields due to their multifaceted properties and therapeutic importance, better understanding of the mechanisms and cellular consequences resulting from their interaction with cells and cellular compo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	E. Preedia Babu, A. Subastri, A. Suyavaran, K. Premkumar, V. Sujatha, B. Aristatile, Ghedeir M. Alshammari, V. Dharuman, C. Thirunavukkarasu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d0281795e31a4548bc25e5f1173141d3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d0281795e31a4548bc25e5f1173141d3
record_format	dspace
spelling	oai:doaj.org-article:d0281795e31a4548bc25e5f1173141d32021-12-02T16:07:01ZSize Dependent Uptake and Hemolytic Effect of Zinc Oxide Nanoparticles on Erythrocytes and Biomedical Potential of ZnO-Ferulic acid Conjugates10.1038/s41598-017-04440-y2045-2322https://doaj.org/article/d0281795e31a4548bc25e5f1173141d32017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04440-yhttps://doaj.org/toc/2045-2322Abstract Despite zinc oxide nanoparticles (ZnONPs) being increasingly used as carriers in biomedical fields due to their multifaceted properties and therapeutic importance, better understanding of the mechanisms and cellular consequences resulting from their interaction with cells and cellular components has been warranted. In the present study, we investigate the size-dependent interaction of ZnONPs on RBCs, and its impact on cell viability, DNA damage, ROS generation and morphological changes, employing cellular and analytical methods. Size, charge, stability and solubility were confirmed by DLS, zeta potential, ICP-AES and TEM analysis. Further ICP-AES, TEM, spectroscopic observations and cell based assays showed that ZnONPs exhibited a size dependent impact on RBCs and haemoglobin (Hb), particularly size <50 nm. Conversely, ferulic acid (FA) conjugates and serum albumin significantly reduced the adverse effects exhibited by ZnONPs. The extent of DNA damage and ROS generation is comparatively low in ZnONPs-FA than in ZnONPs alone treated cells. Thus our study documents a novel conceptualization delineating the influence of size on the material properties and therapeutic potential of nanoparticle.E. Preedia BabuA. SubastriA. SuyavaranK. PremkumarV. SujathaB. AristatileGhedeir M. AlshammariV. DharumanC. ThirunavukkarasuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q E. Preedia Babu A. Subastri A. Suyavaran K. Premkumar V. Sujatha B. Aristatile Ghedeir M. Alshammari V. Dharuman C. Thirunavukkarasu Size Dependent Uptake and Hemolytic Effect of Zinc Oxide Nanoparticles on Erythrocytes and Biomedical Potential of ZnO-Ferulic acid Conjugates
description	Abstract Despite zinc oxide nanoparticles (ZnONPs) being increasingly used as carriers in biomedical fields due to their multifaceted properties and therapeutic importance, better understanding of the mechanisms and cellular consequences resulting from their interaction with cells and cellular components has been warranted. In the present study, we investigate the size-dependent interaction of ZnONPs on RBCs, and its impact on cell viability, DNA damage, ROS generation and morphological changes, employing cellular and analytical methods. Size, charge, stability and solubility were confirmed by DLS, zeta potential, ICP-AES and TEM analysis. Further ICP-AES, TEM, spectroscopic observations and cell based assays showed that ZnONPs exhibited a size dependent impact on RBCs and haemoglobin (Hb), particularly size <50 nm. Conversely, ferulic acid (FA) conjugates and serum albumin significantly reduced the adverse effects exhibited by ZnONPs. The extent of DNA damage and ROS generation is comparatively low in ZnONPs-FA than in ZnONPs alone treated cells. Thus our study documents a novel conceptualization delineating the influence of size on the material properties and therapeutic potential of nanoparticle.
format	article
author	E. Preedia Babu A. Subastri A. Suyavaran K. Premkumar V. Sujatha B. Aristatile Ghedeir M. Alshammari V. Dharuman C. Thirunavukkarasu
author_facet	E. Preedia Babu A. Subastri A. Suyavaran K. Premkumar V. Sujatha B. Aristatile Ghedeir M. Alshammari V. Dharuman C. Thirunavukkarasu
author_sort	E. Preedia Babu
title	Size Dependent Uptake and Hemolytic Effect of Zinc Oxide Nanoparticles on Erythrocytes and Biomedical Potential of ZnO-Ferulic acid Conjugates
title_short	Size Dependent Uptake and Hemolytic Effect of Zinc Oxide Nanoparticles on Erythrocytes and Biomedical Potential of ZnO-Ferulic acid Conjugates
title_full	Size Dependent Uptake and Hemolytic Effect of Zinc Oxide Nanoparticles on Erythrocytes and Biomedical Potential of ZnO-Ferulic acid Conjugates
title_fullStr	Size Dependent Uptake and Hemolytic Effect of Zinc Oxide Nanoparticles on Erythrocytes and Biomedical Potential of ZnO-Ferulic acid Conjugates
title_full_unstemmed	Size Dependent Uptake and Hemolytic Effect of Zinc Oxide Nanoparticles on Erythrocytes and Biomedical Potential of ZnO-Ferulic acid Conjugates
title_sort	size dependent uptake and hemolytic effect of zinc oxide nanoparticles on erythrocytes and biomedical potential of zno-ferulic acid conjugates
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/d0281795e31a4548bc25e5f1173141d3
work_keys_str_mv	AT epreediababu sizedependentuptakeandhemolyticeffectofzincoxidenanoparticlesonerythrocytesandbiomedicalpotentialofznoferulicacidconjugates AT asubastri sizedependentuptakeandhemolyticeffectofzincoxidenanoparticlesonerythrocytesandbiomedicalpotentialofznoferulicacidconjugates AT asuyavaran sizedependentuptakeandhemolyticeffectofzincoxidenanoparticlesonerythrocytesandbiomedicalpotentialofznoferulicacidconjugates AT kpremkumar sizedependentuptakeandhemolyticeffectofzincoxidenanoparticlesonerythrocytesandbiomedicalpotentialofznoferulicacidconjugates AT vsujatha sizedependentuptakeandhemolyticeffectofzincoxidenanoparticlesonerythrocytesandbiomedicalpotentialofznoferulicacidconjugates AT baristatile sizedependentuptakeandhemolyticeffectofzincoxidenanoparticlesonerythrocytesandbiomedicalpotentialofznoferulicacidconjugates AT ghedeirmalshammari sizedependentuptakeandhemolyticeffectofzincoxidenanoparticlesonerythrocytesandbiomedicalpotentialofznoferulicacidconjugates AT vdharuman sizedependentuptakeandhemolyticeffectofzincoxidenanoparticlesonerythrocytesandbiomedicalpotentialofznoferulicacidconjugates AT cthirunavukkarasu sizedependentuptakeandhemolyticeffectofzincoxidenanoparticlesonerythrocytesandbiomedicalpotentialofznoferulicacidconjugates
_version_	1718384806566297600

Size Dependent Uptake and Hemolytic Effect of Zinc Oxide Nanoparticles on Erythrocytes and Biomedical Potential of ZnO-Ferulic acid Conjugates

Ejemplares similares