Characterization of the Pig Gut Microbiome and Antibiotic Resistome in Industrialized Feedlots in China
ABSTRACT To characterize the diversity and richness and explore the function and structure of swine gut microbiome and resistome in common pig-farming feedlots, we sampled and metagenomic sequenced the feces of pigs from four different industrialized feedlots located in four distant provinces across...
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American Society for Microbiology
2019
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oai:doaj.org-article:d02ac5b201544119b9f757187f1fb9ce2021-12-02T19:47:35ZCharacterization of the Pig Gut Microbiome and Antibiotic Resistome in Industrialized Feedlots in China10.1128/mSystems.00206-192379-5077https://doaj.org/article/d02ac5b201544119b9f757187f1fb9ce2019-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00206-19https://doaj.org/toc/2379-5077ABSTRACT To characterize the diversity and richness and explore the function and structure of swine gut microbiome and resistome in common pig-farming feedlots, we sampled and metagenomic sequenced the feces of pigs from four different industrialized feedlots located in four distant provinces across China. Surprisingly, more than half of the nonredundant genes (1,937,648, 54.3%) in the current catalogue were newly found compared with the previously published reference gene catalogue (RGC) of the pig gut microbiome. Additionally, 16 high-completeness draft genomes were obtained by analyzing the dominant species on each feedlot. Notably, seven of these species often appeared in the human body sites. Despite a smaller number of nonredundant genes, our study identified more antibiotic resistance genes than those available in the RGC. Tetracycline, aminoglycoside, and multidrug resistance genes accounted for nearly 70% of the relative abundance in the current catalogue. Slightly higher sharing ratios were shown between the industrialized feedlot pig gut microbiomes and human gut microbiomes than that between the RGC and human counterpart (14.7% versus 12.6% in genes and 94.1% versus 87.7% in functional groups, respectively). Furthermore, a remarkably high number of the antibiotic resistance proteins (n =141) were identified to be shared by the pig, human, and mouse resistome, indicating the potential for horizontal transfer of resistance genes. Of the antibiotic resistance proteins shared by pigs and humans, 50 proteins were related to tetracycline resistance, and 49 were related to aminoglycoside resistance. IMPORTANCE The gut microbiota is believed to be closely related to many important physical functions in the host. Comprehensive data on mammalian gut metagenomes has facilitated research on host-microbiome interaction mechanisms, but less is known about pig gut microbiome, especially the gut microbiome in industrialized feedlot pigs, compared with human microbiome. On the other hand, pig production, as an important source of food, is believed to exacerbate the antibiotic resistance in humans due to the abuse of antibiotics in pig production in various parts of the world. This study delineates an intricate picture of swine gut microbiome and antibiotic resistome in industrialized feedlots and may provide insight for the pig producing industry.Chunlai WangPeng LiQiulong YanLiping ChenTiantian LiWanjiang ZhangHe LiChangming ChenXiuyan HanSiyi ZhangMiao XuBo LiXiaoxuan ZhangHongbo NiYufang MaBo DongShenghui LiSiguo LiuAmerican Society for Microbiologyarticleindustrialized feedlotgene cataloguepig gut microbiomeantibiotic resistance geneantibiotic resistomeMicrobiologyQR1-502ENmSystems, Vol 4, Iss 6 (2019) |
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industrialized feedlot gene catalogue pig gut microbiome antibiotic resistance gene antibiotic resistome Microbiology QR1-502 |
| spellingShingle |
industrialized feedlot gene catalogue pig gut microbiome antibiotic resistance gene antibiotic resistome Microbiology QR1-502 Chunlai Wang Peng Li Qiulong Yan Liping Chen Tiantian Li Wanjiang Zhang He Li Changming Chen Xiuyan Han Siyi Zhang Miao Xu Bo Li Xiaoxuan Zhang Hongbo Ni Yufang Ma Bo Dong Shenghui Li Siguo Liu Characterization of the Pig Gut Microbiome and Antibiotic Resistome in Industrialized Feedlots in China |
| description |
ABSTRACT To characterize the diversity and richness and explore the function and structure of swine gut microbiome and resistome in common pig-farming feedlots, we sampled and metagenomic sequenced the feces of pigs from four different industrialized feedlots located in four distant provinces across China. Surprisingly, more than half of the nonredundant genes (1,937,648, 54.3%) in the current catalogue were newly found compared with the previously published reference gene catalogue (RGC) of the pig gut microbiome. Additionally, 16 high-completeness draft genomes were obtained by analyzing the dominant species on each feedlot. Notably, seven of these species often appeared in the human body sites. Despite a smaller number of nonredundant genes, our study identified more antibiotic resistance genes than those available in the RGC. Tetracycline, aminoglycoside, and multidrug resistance genes accounted for nearly 70% of the relative abundance in the current catalogue. Slightly higher sharing ratios were shown between the industrialized feedlot pig gut microbiomes and human gut microbiomes than that between the RGC and human counterpart (14.7% versus 12.6% in genes and 94.1% versus 87.7% in functional groups, respectively). Furthermore, a remarkably high number of the antibiotic resistance proteins (n =141) were identified to be shared by the pig, human, and mouse resistome, indicating the potential for horizontal transfer of resistance genes. Of the antibiotic resistance proteins shared by pigs and humans, 50 proteins were related to tetracycline resistance, and 49 were related to aminoglycoside resistance. IMPORTANCE The gut microbiota is believed to be closely related to many important physical functions in the host. Comprehensive data on mammalian gut metagenomes has facilitated research on host-microbiome interaction mechanisms, but less is known about pig gut microbiome, especially the gut microbiome in industrialized feedlot pigs, compared with human microbiome. On the other hand, pig production, as an important source of food, is believed to exacerbate the antibiotic resistance in humans due to the abuse of antibiotics in pig production in various parts of the world. This study delineates an intricate picture of swine gut microbiome and antibiotic resistome in industrialized feedlots and may provide insight for the pig producing industry. |
| format |
article |
| author |
Chunlai Wang Peng Li Qiulong Yan Liping Chen Tiantian Li Wanjiang Zhang He Li Changming Chen Xiuyan Han Siyi Zhang Miao Xu Bo Li Xiaoxuan Zhang Hongbo Ni Yufang Ma Bo Dong Shenghui Li Siguo Liu |
| author_facet |
Chunlai Wang Peng Li Qiulong Yan Liping Chen Tiantian Li Wanjiang Zhang He Li Changming Chen Xiuyan Han Siyi Zhang Miao Xu Bo Li Xiaoxuan Zhang Hongbo Ni Yufang Ma Bo Dong Shenghui Li Siguo Liu |
| author_sort |
Chunlai Wang |
| title |
Characterization of the Pig Gut Microbiome and Antibiotic Resistome in Industrialized Feedlots in China |
| title_short |
Characterization of the Pig Gut Microbiome and Antibiotic Resistome in Industrialized Feedlots in China |
| title_full |
Characterization of the Pig Gut Microbiome and Antibiotic Resistome in Industrialized Feedlots in China |
| title_fullStr |
Characterization of the Pig Gut Microbiome and Antibiotic Resistome in Industrialized Feedlots in China |
| title_full_unstemmed |
Characterization of the Pig Gut Microbiome and Antibiotic Resistome in Industrialized Feedlots in China |
| title_sort |
characterization of the pig gut microbiome and antibiotic resistome in industrialized feedlots in china |
| publisher |
American Society for Microbiology |
| publishDate |
2019 |
| url |
https://doaj.org/article/d02ac5b201544119b9f757187f1fb9ce |
| work_keys_str_mv |
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| _version_ |
1718375991218274304 |