Divergence of bacterial communities in the lower airways of CF patients in early childhood.

<h4>Rationale</h4>Chronic airway infection and inflammation resulting in progressive, obstructive lung disease is the leading cause of morbidity and mortality in cystic fibrosis. Understanding the lower airway microbiota across the ages can provide valuable insight and potential therapeu...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	John B O'Connor, Madison M Mottlowitz, Brandie D Wagner, Kathleen L Boyne, Mark J Stevens, Charles E Robertson, Jonathan K Harris, Theresa A Laguna
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d0321265d8184464ad546af551bcee45
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d0321265d8184464ad546af551bcee45
record_format	dspace
spelling	oai:doaj.org-article:d0321265d8184464ad546af551bcee452021-12-02T20:17:18ZDivergence of bacterial communities in the lower airways of CF patients in early childhood.1932-620310.1371/journal.pone.0257838https://doaj.org/article/d0321265d8184464ad546af551bcee452021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257838https://doaj.org/toc/1932-6203<h4>Rationale</h4>Chronic airway infection and inflammation resulting in progressive, obstructive lung disease is the leading cause of morbidity and mortality in cystic fibrosis. Understanding the lower airway microbiota across the ages can provide valuable insight and potential therapeutic targets.<h4>Objectives</h4>To characterize and compare the lower airway microbiota in cystic fibrosis and disease control subjects across the pediatric age spectrum.<h4>Methods</h4>Bronchoalveolar lavage fluid samples from 191 subjects (63 with cystic fibrosis) aged 0 to 21 years were collected along with relevant clinical data. We measured total bacterial load using quantitative polymerase chain reaction and performed 16S rRNA gene sequencing to characterize bacterial communities with species-level sensitivity for select genera. Clinical comparisons were investigated.<h4>Measurements and main results</h4>Cystic fibrosis samples had higher total bacterial load and lower microbial diversity, with a divergence from disease controls around 2-5 years of age, as well as higher neutrophilic inflammation relative to bacterial burden. Cystic fibrosis samples had increased abundance of traditional cystic fibrosis pathogens and decreased abundance of the Streptococcus mitis species group in older subjects. Interestingly, increased diversity in the heterogeneous disease controls was independent of diagnosis and indication. Sequencing was more sensitive than culture, and antibiotic exposure was more common in disease controls, which showed a negative relationship with load and neutrophilic inflammation.<h4>Conclusions</h4>Analysis of lower airway samples from people with cystic fibrosis and disease controls across the ages revealed key differences in airway microbiota and inflammation. The divergence in subjects during early childhood may represent a window of opportunity for intervention and additional study.John B O'ConnorMadison M MottlowitzBrandie D WagnerKathleen L BoyneMark J StevensCharles E RobertsonJonathan K HarrisTheresa A LagunaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 10, p e0257838 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q John B O'Connor Madison M Mottlowitz Brandie D Wagner Kathleen L Boyne Mark J Stevens Charles E Robertson Jonathan K Harris Theresa A Laguna Divergence of bacterial communities in the lower airways of CF patients in early childhood.
description	<h4>Rationale</h4>Chronic airway infection and inflammation resulting in progressive, obstructive lung disease is the leading cause of morbidity and mortality in cystic fibrosis. Understanding the lower airway microbiota across the ages can provide valuable insight and potential therapeutic targets.<h4>Objectives</h4>To characterize and compare the lower airway microbiota in cystic fibrosis and disease control subjects across the pediatric age spectrum.<h4>Methods</h4>Bronchoalveolar lavage fluid samples from 191 subjects (63 with cystic fibrosis) aged 0 to 21 years were collected along with relevant clinical data. We measured total bacterial load using quantitative polymerase chain reaction and performed 16S rRNA gene sequencing to characterize bacterial communities with species-level sensitivity for select genera. Clinical comparisons were investigated.<h4>Measurements and main results</h4>Cystic fibrosis samples had higher total bacterial load and lower microbial diversity, with a divergence from disease controls around 2-5 years of age, as well as higher neutrophilic inflammation relative to bacterial burden. Cystic fibrosis samples had increased abundance of traditional cystic fibrosis pathogens and decreased abundance of the Streptococcus mitis species group in older subjects. Interestingly, increased diversity in the heterogeneous disease controls was independent of diagnosis and indication. Sequencing was more sensitive than culture, and antibiotic exposure was more common in disease controls, which showed a negative relationship with load and neutrophilic inflammation.<h4>Conclusions</h4>Analysis of lower airway samples from people with cystic fibrosis and disease controls across the ages revealed key differences in airway microbiota and inflammation. The divergence in subjects during early childhood may represent a window of opportunity for intervention and additional study.
format	article
author	John B O'Connor Madison M Mottlowitz Brandie D Wagner Kathleen L Boyne Mark J Stevens Charles E Robertson Jonathan K Harris Theresa A Laguna
author_facet	John B O'Connor Madison M Mottlowitz Brandie D Wagner Kathleen L Boyne Mark J Stevens Charles E Robertson Jonathan K Harris Theresa A Laguna
author_sort	John B O'Connor
title	Divergence of bacterial communities in the lower airways of CF patients in early childhood.
title_short	Divergence of bacterial communities in the lower airways of CF patients in early childhood.
title_full	Divergence of bacterial communities in the lower airways of CF patients in early childhood.
title_fullStr	Divergence of bacterial communities in the lower airways of CF patients in early childhood.
title_full_unstemmed	Divergence of bacterial communities in the lower airways of CF patients in early childhood.
title_sort	divergence of bacterial communities in the lower airways of cf patients in early childhood.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/d0321265d8184464ad546af551bcee45
work_keys_str_mv	AT johnboconnor divergenceofbacterialcommunitiesinthelowerairwaysofcfpatientsinearlychildhood AT madisonmmottlowitz divergenceofbacterialcommunitiesinthelowerairwaysofcfpatientsinearlychildhood AT brandiedwagner divergenceofbacterialcommunitiesinthelowerairwaysofcfpatientsinearlychildhood AT kathleenlboyne divergenceofbacterialcommunitiesinthelowerairwaysofcfpatientsinearlychildhood AT markjstevens divergenceofbacterialcommunitiesinthelowerairwaysofcfpatientsinearlychildhood AT charleserobertson divergenceofbacterialcommunitiesinthelowerairwaysofcfpatientsinearlychildhood AT jonathankharris divergenceofbacterialcommunitiesinthelowerairwaysofcfpatientsinearlychildhood AT theresaalaguna divergenceofbacterialcommunitiesinthelowerairwaysofcfpatientsinearlychildhood
_version_	1718374431808552960

Divergence of bacterial communities in the lower airways of CF patients in early childhood.

Ejemplares similares