Assessment of copy number in protooncogenes are predictive of poor survival in advanced gastric cancer

Abstract The copy number (CN) gain of protooncogenes is a frequent finding in gastric carcinoma (GC), but its prognostic implication remains elusive. The study aimed to characterize the clinicopathological features, including prognosis, of GCs with copy number gains in multiple protooncogenes. Three...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Meihui Li, Younghoon Kim, Tae-Shin Kim, Nam-Yun Cho, Jeong Mo Bae, Woo Ho Kim, Gyeong Hoon Kang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d0385f03259c451d86b5bed49fb52bfa
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:d0385f03259c451d86b5bed49fb52bfa
record_format	dspace
spelling	oai:doaj.org-article:d0385f03259c451d86b5bed49fb52bfa2021-12-02T17:52:12ZAssessment of copy number in protooncogenes are predictive of poor survival in advanced gastric cancer10.1038/s41598-021-91652-y2045-2322https://doaj.org/article/d0385f03259c451d86b5bed49fb52bfa2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-91652-yhttps://doaj.org/toc/2045-2322Abstract The copy number (CN) gain of protooncogenes is a frequent finding in gastric carcinoma (GC), but its prognostic implication remains elusive. The study aimed to characterize the clinicopathological features, including prognosis, of GCs with copy number gains in multiple protooncogenes. Three hundred thirty-three patients with advanced GC were analyzed for their gene ratios in EGFR, GATA6, IGF2, and SETDB1 using droplet dPCR (ddPCR) for an accurate assessment of CN changes in target genes. The number of GC patients with 3 or more genes with CN gain was 16 (4.8%). Compared with the GCs with 2 or less genes with CN gain, the GCs with 3 or more CN gains displayed more frequent venous invasion, a lower density of tumor-infiltrating lymphocytes, and lower methylation levels of L1 or SAT-alpha. Microsatellite instability-high tumors or Epstein–Barr virus-positive tumors were not found in the GCs with 3 or more genes with CN gain. Patients of this groups also showed the worst clinical outcomes for both overall survival and recurrence-free survival, which was persistent in the multivariate survival analyses. Our findings suggest that the ddPCR-based detection of multiple CN gain of protooncogenes might help to identify a subset of patients with poor prognosis.Meihui LiYounghoon KimTae-Shin KimNam-Yun ChoJeong Mo BaeWoo Ho KimGyeong Hoon KangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Meihui Li Younghoon Kim Tae-Shin Kim Nam-Yun Cho Jeong Mo Bae Woo Ho Kim Gyeong Hoon Kang Assessment of copy number in protooncogenes are predictive of poor survival in advanced gastric cancer
description	Abstract The copy number (CN) gain of protooncogenes is a frequent finding in gastric carcinoma (GC), but its prognostic implication remains elusive. The study aimed to characterize the clinicopathological features, including prognosis, of GCs with copy number gains in multiple protooncogenes. Three hundred thirty-three patients with advanced GC were analyzed for their gene ratios in EGFR, GATA6, IGF2, and SETDB1 using droplet dPCR (ddPCR) for an accurate assessment of CN changes in target genes. The number of GC patients with 3 or more genes with CN gain was 16 (4.8%). Compared with the GCs with 2 or less genes with CN gain, the GCs with 3 or more CN gains displayed more frequent venous invasion, a lower density of tumor-infiltrating lymphocytes, and lower methylation levels of L1 or SAT-alpha. Microsatellite instability-high tumors or Epstein–Barr virus-positive tumors were not found in the GCs with 3 or more genes with CN gain. Patients of this groups also showed the worst clinical outcomes for both overall survival and recurrence-free survival, which was persistent in the multivariate survival analyses. Our findings suggest that the ddPCR-based detection of multiple CN gain of protooncogenes might help to identify a subset of patients with poor prognosis.
format	article
author	Meihui Li Younghoon Kim Tae-Shin Kim Nam-Yun Cho Jeong Mo Bae Woo Ho Kim Gyeong Hoon Kang
author_facet	Meihui Li Younghoon Kim Tae-Shin Kim Nam-Yun Cho Jeong Mo Bae Woo Ho Kim Gyeong Hoon Kang
author_sort	Meihui Li
title	Assessment of copy number in protooncogenes are predictive of poor survival in advanced gastric cancer
title_short	Assessment of copy number in protooncogenes are predictive of poor survival in advanced gastric cancer
title_full	Assessment of copy number in protooncogenes are predictive of poor survival in advanced gastric cancer
title_fullStr	Assessment of copy number in protooncogenes are predictive of poor survival in advanced gastric cancer
title_full_unstemmed	Assessment of copy number in protooncogenes are predictive of poor survival in advanced gastric cancer
title_sort	assessment of copy number in protooncogenes are predictive of poor survival in advanced gastric cancer
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/d0385f03259c451d86b5bed49fb52bfa
work_keys_str_mv	AT meihuili assessmentofcopynumberinprotooncogenesarepredictiveofpoorsurvivalinadvancedgastriccancer AT younghoonkim assessmentofcopynumberinprotooncogenesarepredictiveofpoorsurvivalinadvancedgastriccancer AT taeshinkim assessmentofcopynumberinprotooncogenesarepredictiveofpoorsurvivalinadvancedgastriccancer AT namyuncho assessmentofcopynumberinprotooncogenesarepredictiveofpoorsurvivalinadvancedgastriccancer AT jeongmobae assessmentofcopynumberinprotooncogenesarepredictiveofpoorsurvivalinadvancedgastriccancer AT woohokim assessmentofcopynumberinprotooncogenesarepredictiveofpoorsurvivalinadvancedgastriccancer AT gyeonghoonkang assessmentofcopynumberinprotooncogenesarepredictiveofpoorsurvivalinadvancedgastriccancer
_version_	1718379254202236928

Assessment of copy number in protooncogenes are predictive of poor survival in advanced gastric cancer

Ejemplares similares