Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations

Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations

Generally, fluorescence imaging needs to be done in a dark environment using molecules with spectrally separated emissions. Here, Quérard et al. develop a protocol for high-speed imaging and remote sensing of spectrally overlapping reversible photoswitchable fluorophores in ambient light.

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Detalles Bibliográficos
Autores principales: Jérôme Quérard, Ruikang Zhang, Zsolt Kelemen, Marie-Aude Plamont, Xiaojiang Xie, Raja Chouket, Insa Roemgens, Yulia Korepina, Samantha Albright, Eliane Ipendey, Michel Volovitch, Hanna L. Sladitschek, Pierre Neveu, Lionel Gissot, Arnaud Gautier, Jean-Denis Faure, Vincent Croquette, Thomas Le Saux, Ludovic Jullien
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
Materias:
Science
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Acceso en línea:https://doaj.org/article/d03b095697884916b74ba61ec21cc7f5
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Descripción
Sumario:Generally, fluorescence imaging needs to be done in a dark environment using molecules with spectrally separated emissions. Here, Quérard et al. develop a protocol for high-speed imaging and remote sensing of spectrally overlapping reversible photoswitchable fluorophores in ambient light.

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