Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations

Generally, fluorescence imaging needs to be done in a dark environment using molecules with spectrally separated emissions. Here, Quérard et al. develop a protocol for high-speed imaging and remote sensing of spectrally overlapping reversible photoswitchable fluorophores in ambient light.

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Autores principales: Jérôme Quérard, Ruikang Zhang, Zsolt Kelemen, Marie-Aude Plamont, Xiaojiang Xie, Raja Chouket, Insa Roemgens, Yulia Korepina, Samantha Albright, Eliane Ipendey, Michel Volovitch, Hanna L. Sladitschek, Pierre Neveu, Lionel Gissot, Arnaud Gautier, Jean-Denis Faure, Vincent Croquette, Thomas Le Saux, Ludovic Jullien
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Lenguaje:EN
Publicado: Nature Portfolio 2017
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Acceso en línea:https://doaj.org/article/d03b095697884916b74ba61ec21cc7f5
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spelling oai:doaj.org-article:d03b095697884916b74ba61ec21cc7f52021-12-02T16:51:52ZResonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations10.1038/s41467-017-00847-32041-1723https://doaj.org/article/d03b095697884916b74ba61ec21cc7f52017-10-01T00:00:00Zhttps://doi.org/10.1038/s41467-017-00847-3https://doaj.org/toc/2041-1723Generally, fluorescence imaging needs to be done in a dark environment using molecules with spectrally separated emissions. Here, Quérard et al. develop a protocol for high-speed imaging and remote sensing of spectrally overlapping reversible photoswitchable fluorophores in ambient light.Jérôme QuérardRuikang ZhangZsolt KelemenMarie-Aude PlamontXiaojiang XieRaja ChouketInsa RoemgensYulia KorepinaSamantha AlbrightEliane IpendeyMichel VolovitchHanna L. SladitschekPierre NeveuLionel GissotArnaud GautierJean-Denis FaureVincent CroquetteThomas Le SauxLudovic JullienNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-8 (2017)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Jérôme Quérard
Ruikang Zhang
Zsolt Kelemen
Marie-Aude Plamont
Xiaojiang Xie
Raja Chouket
Insa Roemgens
Yulia Korepina
Samantha Albright
Eliane Ipendey
Michel Volovitch
Hanna L. Sladitschek
Pierre Neveu
Lionel Gissot
Arnaud Gautier
Jean-Denis Faure
Vincent Croquette
Thomas Le Saux
Ludovic Jullien
Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations
description Generally, fluorescence imaging needs to be done in a dark environment using molecules with spectrally separated emissions. Here, Quérard et al. develop a protocol for high-speed imaging and remote sensing of spectrally overlapping reversible photoswitchable fluorophores in ambient light.
format article
author Jérôme Quérard
Ruikang Zhang
Zsolt Kelemen
Marie-Aude Plamont
Xiaojiang Xie
Raja Chouket
Insa Roemgens
Yulia Korepina
Samantha Albright
Eliane Ipendey
Michel Volovitch
Hanna L. Sladitschek
Pierre Neveu
Lionel Gissot
Arnaud Gautier
Jean-Denis Faure
Vincent Croquette
Thomas Le Saux
Ludovic Jullien
author_facet Jérôme Quérard
Ruikang Zhang
Zsolt Kelemen
Marie-Aude Plamont
Xiaojiang Xie
Raja Chouket
Insa Roemgens
Yulia Korepina
Samantha Albright
Eliane Ipendey
Michel Volovitch
Hanna L. Sladitschek
Pierre Neveu
Lionel Gissot
Arnaud Gautier
Jean-Denis Faure
Vincent Croquette
Thomas Le Saux
Ludovic Jullien
author_sort Jérôme Quérard
title Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations
title_short Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations
title_full Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations
title_fullStr Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations
title_full_unstemmed Resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations
title_sort resonant out-of-phase fluorescence microscopy and remote imaging overcome spectral limitations
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/d03b095697884916b74ba61ec21cc7f5
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