MicroRNA-145 targets YES and STAT1 in colon cancer cells.

<h4>Background</h4>MicroRNAs (miRNAs) have emerged as important gene regulators and are recognized as key players in tumorigenesis. miR-145 is reported to be down-regulated in several cancers, but knowledge of its targets in colon cancer remains limited.<h4>Methodology/principal fi...

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Autores principales: Lea H Gregersen, Anders B Jacobsen, Lisa B Frankel, Jiayu Wen, Anders Krogh, Anders H Lund
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Publicado: Public Library of Science (PLoS) 2010
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Acceso en línea:https://doaj.org/article/d03cd24fb2eb43119c3b7ca7774005f5
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spelling oai:doaj.org-article:d03cd24fb2eb43119c3b7ca7774005f52021-11-25T06:26:29ZMicroRNA-145 targets YES and STAT1 in colon cancer cells.1932-620310.1371/journal.pone.0008836https://doaj.org/article/d03cd24fb2eb43119c3b7ca7774005f52010-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20098684/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>MicroRNAs (miRNAs) have emerged as important gene regulators and are recognized as key players in tumorigenesis. miR-145 is reported to be down-regulated in several cancers, but knowledge of its targets in colon cancer remains limited.<h4>Methodology/principal findings</h4>To investigate the role of miR-145 in colon cancer, we have employed a microarray based approach to identify miR-145 targets. Based on seed site enrichment analyses and unbiased word analyses, we found a significant enrichment of miRNA binding sites in the 3'-untranslated regions (UTRs) of transcripts down-regulated upon miRNA overexpression. Gene Ontology analysis showed an overrepresentation of genes involved in cell death, cellular growth and proliferation, cell cycle, gene expression and cancer. A number of the identified miRNA targets have previously been implicated in cancer, including YES, FSCN1, ADAM17, BIRC2, VANGL1 as well as the transcription factor STAT1. Both YES and STAT1 were verified as direct miR-145 targets.<h4>Conclusions/significance</h4>The study identifies and validates new cancer-relevant direct targets of miR-145 in colon cancer cells and hereby adds important mechanistic understanding of the tumor-suppressive functions of miR-145.Lea H GregersenAnders B JacobsenLisa B FrankelJiayu WenAnders KroghAnders H LundPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 1, p e8836 (2010)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Lea H Gregersen
Anders B Jacobsen
Lisa B Frankel
Jiayu Wen
Anders Krogh
Anders H Lund
MicroRNA-145 targets YES and STAT1 in colon cancer cells.
description <h4>Background</h4>MicroRNAs (miRNAs) have emerged as important gene regulators and are recognized as key players in tumorigenesis. miR-145 is reported to be down-regulated in several cancers, but knowledge of its targets in colon cancer remains limited.<h4>Methodology/principal findings</h4>To investigate the role of miR-145 in colon cancer, we have employed a microarray based approach to identify miR-145 targets. Based on seed site enrichment analyses and unbiased word analyses, we found a significant enrichment of miRNA binding sites in the 3'-untranslated regions (UTRs) of transcripts down-regulated upon miRNA overexpression. Gene Ontology analysis showed an overrepresentation of genes involved in cell death, cellular growth and proliferation, cell cycle, gene expression and cancer. A number of the identified miRNA targets have previously been implicated in cancer, including YES, FSCN1, ADAM17, BIRC2, VANGL1 as well as the transcription factor STAT1. Both YES and STAT1 were verified as direct miR-145 targets.<h4>Conclusions/significance</h4>The study identifies and validates new cancer-relevant direct targets of miR-145 in colon cancer cells and hereby adds important mechanistic understanding of the tumor-suppressive functions of miR-145.
format article
author Lea H Gregersen
Anders B Jacobsen
Lisa B Frankel
Jiayu Wen
Anders Krogh
Anders H Lund
author_facet Lea H Gregersen
Anders B Jacobsen
Lisa B Frankel
Jiayu Wen
Anders Krogh
Anders H Lund
author_sort Lea H Gregersen
title MicroRNA-145 targets YES and STAT1 in colon cancer cells.
title_short MicroRNA-145 targets YES and STAT1 in colon cancer cells.
title_full MicroRNA-145 targets YES and STAT1 in colon cancer cells.
title_fullStr MicroRNA-145 targets YES and STAT1 in colon cancer cells.
title_full_unstemmed MicroRNA-145 targets YES and STAT1 in colon cancer cells.
title_sort microrna-145 targets yes and stat1 in colon cancer cells.
publisher Public Library of Science (PLoS)
publishDate 2010
url https://doaj.org/article/d03cd24fb2eb43119c3b7ca7774005f5
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AT andersbjacobsen microrna145targetsyesandstat1incoloncancercells
AT lisabfrankel microrna145targetsyesandstat1incoloncancercells
AT jiayuwen microrna145targetsyesandstat1incoloncancercells
AT anderskrogh microrna145targetsyesandstat1incoloncancercells
AT andershlund microrna145targetsyesandstat1incoloncancercells
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