Probiotic Enhancement of Antioxidant Capacity and Alterations of Gut Microbiota Composition in 6-Hydroxydopamin-Induced Parkinson’s Disease Rats

Oxidative stress plays a key role in the degeneration of dopaminergic neurons in Parkinson’s disease (PD), which may be aggravated by concomitant PD-associated gut dysbiosis. Probiotics and prebiotics are therapeutically relevant to these conditions due to their antioxidant, anti-inflammatory, and g...

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Autores principales:	Shu-Ping Tsao, Bira Arumndari Nurrahma, Ravi Kumar, Chieh-Hsi Wu, Tu-Hsueh Yeh, Ching-Chi Chiu, Yen-Peng Lee, Yi-Chi Liao, Cheng-Hsieh Huang, Yao-Tsung Yeh, Hui-Yu Huang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	probiotics neuroprotection fecal microbiota composition antioxidant activities Parkin-son’s disease Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/d040fdd4b546411cb01e2ec8ca100a10
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spelling	oai:doaj.org-article:d040fdd4b546411cb01e2ec8ca100a102021-11-25T16:29:09ZProbiotic Enhancement of Antioxidant Capacity and Alterations of Gut Microbiota Composition in 6-Hydroxydopamin-Induced Parkinson’s Disease Rats10.3390/antiox101118232076-3921https://doaj.org/article/d040fdd4b546411cb01e2ec8ca100a102021-11-01T00:00:00Zhttps://www.mdpi.com/2076-3921/10/11/1823https://doaj.org/toc/2076-3921Oxidative stress plays a key role in the degeneration of dopaminergic neurons in Parkinson’s disease (PD), which may be aggravated by concomitant PD-associated gut dysbiosis. Probiotics and prebiotics are therapeutically relevant to these conditions due to their antioxidant, anti-inflammatory, and gut microbiome modulation properties. However, the mechanisms by which probiotic/prebiotic supplementation affects antioxidant capacity and the gut microbiome in PD remains poorly characterized. In this study, we assessed the effects of a <i>Lactobacillus salivarius</i> AP-32 probiotic, a prebiotic (dried AP-32 culture medium supernatant), and a probiotic/prebiotic cocktail in rats with unilateral 6-hydroxydopamine (6-OHDA)-induced PD. The neuroprotective effects and levels of oxidative stress were evaluated after eight weeks of daily supplementation. Fecal microbiota composition was analyzed by fecal 16S rRNA gene sequencing. The supplements were associated with direct increases in host antioxidant enzyme activities and short-chain fatty acid production, protected dopaminergic neurons, and improved motor functions. The supplements also altered the fecal microbiota composition, and some specifically enriched commensal taxa correlated positively with superoxide dismutase, glutathione peroxidase, and catalase activity, indicating supplementation also promotes antioxidant activity via an indirect pathway. Therefore, <i>L. salivarius</i> AP-32 supplementation enhanced the activity of host antioxidant enzymes via direct and indirect modes of action in rats with 6-OHDA-induced PD.Shu-Ping TsaoBira Arumndari NurrahmaRavi KumarChieh-Hsi WuTu-Hsueh YehChing-Chi ChiuYen-Peng LeeYi-Chi LiaoCheng-Hsieh HuangYao-Tsung YehHui-Yu HuangMDPI AGarticleprobioticsneuroprotectionfecal microbiota compositionantioxidant activitiesParkin-son’s diseaseTherapeutics. PharmacologyRM1-950ENAntioxidants, Vol 10, Iss 1823, p 1823 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	probiotics neuroprotection fecal microbiota composition antioxidant activities Parkin-son’s disease Therapeutics. Pharmacology RM1-950
spellingShingle	probiotics neuroprotection fecal microbiota composition antioxidant activities Parkin-son’s disease Therapeutics. Pharmacology RM1-950 Shu-Ping Tsao Bira Arumndari Nurrahma Ravi Kumar Chieh-Hsi Wu Tu-Hsueh Yeh Ching-Chi Chiu Yen-Peng Lee Yi-Chi Liao Cheng-Hsieh Huang Yao-Tsung Yeh Hui-Yu Huang Probiotic Enhancement of Antioxidant Capacity and Alterations of Gut Microbiota Composition in 6-Hydroxydopamin-Induced Parkinson’s Disease Rats
description	Oxidative stress plays a key role in the degeneration of dopaminergic neurons in Parkinson’s disease (PD), which may be aggravated by concomitant PD-associated gut dysbiosis. Probiotics and prebiotics are therapeutically relevant to these conditions due to their antioxidant, anti-inflammatory, and gut microbiome modulation properties. However, the mechanisms by which probiotic/prebiotic supplementation affects antioxidant capacity and the gut microbiome in PD remains poorly characterized. In this study, we assessed the effects of a <i>Lactobacillus salivarius</i> AP-32 probiotic, a prebiotic (dried AP-32 culture medium supernatant), and a probiotic/prebiotic cocktail in rats with unilateral 6-hydroxydopamine (6-OHDA)-induced PD. The neuroprotective effects and levels of oxidative stress were evaluated after eight weeks of daily supplementation. Fecal microbiota composition was analyzed by fecal 16S rRNA gene sequencing. The supplements were associated with direct increases in host antioxidant enzyme activities and short-chain fatty acid production, protected dopaminergic neurons, and improved motor functions. The supplements also altered the fecal microbiota composition, and some specifically enriched commensal taxa correlated positively with superoxide dismutase, glutathione peroxidase, and catalase activity, indicating supplementation also promotes antioxidant activity via an indirect pathway. Therefore, <i>L. salivarius</i> AP-32 supplementation enhanced the activity of host antioxidant enzymes via direct and indirect modes of action in rats with 6-OHDA-induced PD.
format	article
author	Shu-Ping Tsao Bira Arumndari Nurrahma Ravi Kumar Chieh-Hsi Wu Tu-Hsueh Yeh Ching-Chi Chiu Yen-Peng Lee Yi-Chi Liao Cheng-Hsieh Huang Yao-Tsung Yeh Hui-Yu Huang
author_facet	Shu-Ping Tsao Bira Arumndari Nurrahma Ravi Kumar Chieh-Hsi Wu Tu-Hsueh Yeh Ching-Chi Chiu Yen-Peng Lee Yi-Chi Liao Cheng-Hsieh Huang Yao-Tsung Yeh Hui-Yu Huang
author_sort	Shu-Ping Tsao
title	Probiotic Enhancement of Antioxidant Capacity and Alterations of Gut Microbiota Composition in 6-Hydroxydopamin-Induced Parkinson’s Disease Rats
title_short	Probiotic Enhancement of Antioxidant Capacity and Alterations of Gut Microbiota Composition in 6-Hydroxydopamin-Induced Parkinson’s Disease Rats
title_full	Probiotic Enhancement of Antioxidant Capacity and Alterations of Gut Microbiota Composition in 6-Hydroxydopamin-Induced Parkinson’s Disease Rats
title_fullStr	Probiotic Enhancement of Antioxidant Capacity and Alterations of Gut Microbiota Composition in 6-Hydroxydopamin-Induced Parkinson’s Disease Rats
title_full_unstemmed	Probiotic Enhancement of Antioxidant Capacity and Alterations of Gut Microbiota Composition in 6-Hydroxydopamin-Induced Parkinson’s Disease Rats
title_sort	probiotic enhancement of antioxidant capacity and alterations of gut microbiota composition in 6-hydroxydopamin-induced parkinson’s disease rats
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d040fdd4b546411cb01e2ec8ca100a10
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Probiotic Enhancement of Antioxidant Capacity and Alterations of Gut Microbiota Composition in 6-Hydroxydopamin-Induced Parkinson’s Disease Rats

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