Antimicrobial Drug-Resistant <i>Salmonella</i> in Urban Cats: Is There an Actual Risk to Public Health?

The present study was undertaken to investigate the presence of <i>Salmonella</i> spp. in the faeces of client-owned cats in urban areas and to evaluate the risk that is posed to public health. Fresh faecal samples were collected directly from the rectums from 53 diarrhoeic and 32 non-di...

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Autores principales:	János Dégi, Kálmán Imre, Viorel Herman, Iulia Bucur, Isidora Radulov, Oana-Cătălina Petrec, Romeo Teodor Cristina
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Salmonella</i> zoonotic cats public health Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/d047911ccc5c4f2fa5212e4f3e8f8deb
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spelling	oai:doaj.org-article:d047911ccc5c4f2fa5212e4f3e8f8deb2021-11-25T16:24:51ZAntimicrobial Drug-Resistant <i>Salmonella</i> in Urban Cats: Is There an Actual Risk to Public Health?10.3390/antibiotics101114042079-6382https://doaj.org/article/d047911ccc5c4f2fa5212e4f3e8f8deb2021-11-01T00:00:00Zhttps://www.mdpi.com/2079-6382/10/11/1404https://doaj.org/toc/2079-6382The present study was undertaken to investigate the presence of <i>Salmonella</i> spp. in the faeces of client-owned cats in urban areas and to evaluate the risk that is posed to public health. Fresh faecal samples were collected directly from the rectums from 53 diarrhoeic and 32 non-diarrhoeic cats. The samples were individually screened for the presence of <i>Salmonella</i> spp. using standard methods and, in the case of positive findings, the resulting typical colonies were then biochemically confirmed using the VITEK<sup>®</sup>2 automated system. Subsequently, all of the <i>Salmonella</i> spp. isolates were molecularly tested for the presence of the <i>invA</i> gene. All of the isolates were serotyped using the slide agglutination technique according to the White–Kauffmann–Le Minor scheme. The phenotypic antimicrobial susceptibility profile of the isolated strains was obtained from the VITEK<sup>®</sup>2 system using specific cards from the Gram-negative bacteria. A total of 16 of the samples (18.82%) tested positive for <i>Salmonella</i> spp. according to conventional and molecular testing methods. Serotyping of the <i>Salmonella</i> isolates showed the presence of three serotypes, namely <i>S. enteritidis</i> (<i>n</i> = 9; 56.3%), <i>S. typhimurium</i> (<i>n</i> = 4; 25%), and <i>S. kentucky</i> (<i>n</i> = 3; 18.8%). All of the tested strains showed strong resistance towards cefazolin, cefepime, ceftazidime, and ceftriaxone. Additionally, resistance (listed in descending order of strength) was observed to trimethoprim/sulfamethoxazole (11/16; 68.8%), ampicillin (10/16; 62.5%), ampicillin/sulbactam (9/16; 56.3%), gentamicin (9/16; 56.3%), nitrofurantoin (8/16; 50.0%), and amikacin (5/16; 31.3%). No resistance was expressed against ciprofloxacin, ertapenem, imipenem, levofloxacin, piperacillin/tazobactam, and tobramycin. The results of this study highlight a substantial public health issue and medical concern, especially in vulnerable people, such as children, the elderly, and immunocompromised individuals.János DégiKálmán ImreViorel HermanIulia BucurIsidora RadulovOana-Cătălina PetrecRomeo Teodor CristinaMDPI AGarticle<i>Salmonella</i>zoonoticcatspublic healthTherapeutics. PharmacologyRM1-950ENAntibiotics, Vol 10, Iss 1404, p 1404 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Salmonella</i> zoonotic cats public health Therapeutics. Pharmacology RM1-950
spellingShingle	<i>Salmonella</i> zoonotic cats public health Therapeutics. Pharmacology RM1-950 János Dégi Kálmán Imre Viorel Herman Iulia Bucur Isidora Radulov Oana-Cătălina Petrec Romeo Teodor Cristina Antimicrobial Drug-Resistant <i>Salmonella</i> in Urban Cats: Is There an Actual Risk to Public Health?
description	The present study was undertaken to investigate the presence of <i>Salmonella</i> spp. in the faeces of client-owned cats in urban areas and to evaluate the risk that is posed to public health. Fresh faecal samples were collected directly from the rectums from 53 diarrhoeic and 32 non-diarrhoeic cats. The samples were individually screened for the presence of <i>Salmonella</i> spp. using standard methods and, in the case of positive findings, the resulting typical colonies were then biochemically confirmed using the VITEK<sup>®</sup>2 automated system. Subsequently, all of the <i>Salmonella</i> spp. isolates were molecularly tested for the presence of the <i>invA</i> gene. All of the isolates were serotyped using the slide agglutination technique according to the White–Kauffmann–Le Minor scheme. The phenotypic antimicrobial susceptibility profile of the isolated strains was obtained from the VITEK<sup>®</sup>2 system using specific cards from the Gram-negative bacteria. A total of 16 of the samples (18.82%) tested positive for <i>Salmonella</i> spp. according to conventional and molecular testing methods. Serotyping of the <i>Salmonella</i> isolates showed the presence of three serotypes, namely <i>S. enteritidis</i> (<i>n</i> = 9; 56.3%), <i>S. typhimurium</i> (<i>n</i> = 4; 25%), and <i>S. kentucky</i> (<i>n</i> = 3; 18.8%). All of the tested strains showed strong resistance towards cefazolin, cefepime, ceftazidime, and ceftriaxone. Additionally, resistance (listed in descending order of strength) was observed to trimethoprim/sulfamethoxazole (11/16; 68.8%), ampicillin (10/16; 62.5%), ampicillin/sulbactam (9/16; 56.3%), gentamicin (9/16; 56.3%), nitrofurantoin (8/16; 50.0%), and amikacin (5/16; 31.3%). No resistance was expressed against ciprofloxacin, ertapenem, imipenem, levofloxacin, piperacillin/tazobactam, and tobramycin. The results of this study highlight a substantial public health issue and medical concern, especially in vulnerable people, such as children, the elderly, and immunocompromised individuals.
format	article
author	János Dégi Kálmán Imre Viorel Herman Iulia Bucur Isidora Radulov Oana-Cătălina Petrec Romeo Teodor Cristina
author_facet	János Dégi Kálmán Imre Viorel Herman Iulia Bucur Isidora Radulov Oana-Cătălina Petrec Romeo Teodor Cristina
author_sort	János Dégi
title	Antimicrobial Drug-Resistant <i>Salmonella</i> in Urban Cats: Is There an Actual Risk to Public Health?
title_short	Antimicrobial Drug-Resistant <i>Salmonella</i> in Urban Cats: Is There an Actual Risk to Public Health?
title_full	Antimicrobial Drug-Resistant <i>Salmonella</i> in Urban Cats: Is There an Actual Risk to Public Health?
title_fullStr	Antimicrobial Drug-Resistant <i>Salmonella</i> in Urban Cats: Is There an Actual Risk to Public Health?
title_full_unstemmed	Antimicrobial Drug-Resistant <i>Salmonella</i> in Urban Cats: Is There an Actual Risk to Public Health?
title_sort	antimicrobial drug-resistant <i>salmonella</i> in urban cats: is there an actual risk to public health?
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d047911ccc5c4f2fa5212e4f3e8f8deb
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Antimicrobial Drug-Resistant <i>Salmonella</i> in Urban Cats: Is There an Actual Risk to Public Health?

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