Decrease in CD14++CD16+ Monocytes in Low-Immunological-Risk Kidney Transplant Patients with Subclinical Borderline Inflammation

We determined the association between CD14++CD16+ monocytes and subclinical infiltrates that do not reach the histological threshold for rejection (≥Banff IA). We studied low-immunological-risk kidney-transplant recipients in a clinical trial (NCT02284464; EudraCT 2012-003298-24) whose protocol biop...

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Autores principales:	Abelardo Caballero, Teresa Vazquez-Sanchez, Pedro Ruiz-Esteban, Myriam Leon, Juana Alonso-Titos, Veronica Lopez, Eugenia Sola, Elena Gutierrez, Mercedes Cabello, Cristina Casas-Gonzalez, Rafael Pozo-Alvarez, Juan Delgado-Burgos, Domingo Hernandez
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	kidney transplant monocytes CD14++CD16+ kidney biopsy borderline lesions Medicine R
Acceso en línea:	https://doaj.org/article/d0484b89ac4a422192176ba25494a54c
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spelling	oai:doaj.org-article:d0484b89ac4a422192176ba25494a54c2021-11-11T17:40:22ZDecrease in CD14++CD16+ Monocytes in Low-Immunological-Risk Kidney Transplant Patients with Subclinical Borderline Inflammation10.3390/jcm102150512077-0383https://doaj.org/article/d0484b89ac4a422192176ba25494a54c2021-10-01T00:00:00Zhttps://www.mdpi.com/2077-0383/10/21/5051https://doaj.org/toc/2077-0383We determined the association between CD14++CD16+ monocytes and subclinical infiltrates that do not reach the histological threshold for rejection (≥Banff IA). We studied low-immunological-risk kidney-transplant recipients in a clinical trial (NCT02284464; EudraCT 2012-003298-24) whose protocol biopsy in the third month showed no significant changes or borderline lesions (BL). Flow cytometry was used to analyze the percentage of CD14++CD16+ monocytes in peripheral blood (PB) and blood from a fine-needle-aspiration biopsy (FNAB). A protocol biopsy was performed in 81 low-immunological-risk patients, of whom 15 were excluded (BK polyomavirus and rejection). The 28 (42.4%) with borderline lesions had significantly low levels of CD14++CD16+ in PB compared to patients with normal biopsies (7.9 ± 5.4 vs. 13.0 ± 12.8; <i>p</i> = 0.047). Patients without significant changes had similar percentages of CD14++CD16+ monocytes in the graft blood (GB) and FNAB blood. The percentage of these monocytes in the patients with an interstitial infiltrate, however, increased significantly in the FNAB blood compared to the GB: 16.9 ± 16.6 vs. 7.9 ± 5.4; <i>p</i> = 0.006. A difference of 50% in CD14++CD16+ in the GB versus the PB was a significant risk factor (<i>p</i> = 0.002) for BL, increasing the risk seven times. A decrease in CD14++CD16+ in the PB could be associated with the recruitment of these cells to the graft tissue in cases of subclinical BL inflammatory infiltrates below the threshold for rejection.Abelardo CaballeroTeresa Vazquez-SanchezPedro Ruiz-EstebanMyriam LeonJuana Alonso-TitosVeronica LopezEugenia SolaElena GutierrezMercedes CabelloCristina Casas-GonzalezRafael Pozo-AlvarezJuan Delgado-BurgosDomingo HernandezMDPI AGarticlekidney transplantmonocytesCD14++CD16+kidney biopsyborderline lesionsMedicineRENJournal of Clinical Medicine, Vol 10, Iss 5051, p 5051 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	kidney transplant monocytes CD14++CD16+ kidney biopsy borderline lesions Medicine R
spellingShingle	kidney transplant monocytes CD14++CD16+ kidney biopsy borderline lesions Medicine R Abelardo Caballero Teresa Vazquez-Sanchez Pedro Ruiz-Esteban Myriam Leon Juana Alonso-Titos Veronica Lopez Eugenia Sola Elena Gutierrez Mercedes Cabello Cristina Casas-Gonzalez Rafael Pozo-Alvarez Juan Delgado-Burgos Domingo Hernandez Decrease in CD14++CD16+ Monocytes in Low-Immunological-Risk Kidney Transplant Patients with Subclinical Borderline Inflammation
description	We determined the association between CD14++CD16+ monocytes and subclinical infiltrates that do not reach the histological threshold for rejection (≥Banff IA). We studied low-immunological-risk kidney-transplant recipients in a clinical trial (NCT02284464; EudraCT 2012-003298-24) whose protocol biopsy in the third month showed no significant changes or borderline lesions (BL). Flow cytometry was used to analyze the percentage of CD14++CD16+ monocytes in peripheral blood (PB) and blood from a fine-needle-aspiration biopsy (FNAB). A protocol biopsy was performed in 81 low-immunological-risk patients, of whom 15 were excluded (BK polyomavirus and rejection). The 28 (42.4%) with borderline lesions had significantly low levels of CD14++CD16+ in PB compared to patients with normal biopsies (7.9 ± 5.4 vs. 13.0 ± 12.8; <i>p</i> = 0.047). Patients without significant changes had similar percentages of CD14++CD16+ monocytes in the graft blood (GB) and FNAB blood. The percentage of these monocytes in the patients with an interstitial infiltrate, however, increased significantly in the FNAB blood compared to the GB: 16.9 ± 16.6 vs. 7.9 ± 5.4; <i>p</i> = 0.006. A difference of 50% in CD14++CD16+ in the GB versus the PB was a significant risk factor (<i>p</i> = 0.002) for BL, increasing the risk seven times. A decrease in CD14++CD16+ in the PB could be associated with the recruitment of these cells to the graft tissue in cases of subclinical BL inflammatory infiltrates below the threshold for rejection.
format	article
author	Abelardo Caballero Teresa Vazquez-Sanchez Pedro Ruiz-Esteban Myriam Leon Juana Alonso-Titos Veronica Lopez Eugenia Sola Elena Gutierrez Mercedes Cabello Cristina Casas-Gonzalez Rafael Pozo-Alvarez Juan Delgado-Burgos Domingo Hernandez
author_facet	Abelardo Caballero Teresa Vazquez-Sanchez Pedro Ruiz-Esteban Myriam Leon Juana Alonso-Titos Veronica Lopez Eugenia Sola Elena Gutierrez Mercedes Cabello Cristina Casas-Gonzalez Rafael Pozo-Alvarez Juan Delgado-Burgos Domingo Hernandez
author_sort	Abelardo Caballero
title	Decrease in CD14++CD16+ Monocytes in Low-Immunological-Risk Kidney Transplant Patients with Subclinical Borderline Inflammation
title_short	Decrease in CD14++CD16+ Monocytes in Low-Immunological-Risk Kidney Transplant Patients with Subclinical Borderline Inflammation
title_full	Decrease in CD14++CD16+ Monocytes in Low-Immunological-Risk Kidney Transplant Patients with Subclinical Borderline Inflammation
title_fullStr	Decrease in CD14++CD16+ Monocytes in Low-Immunological-Risk Kidney Transplant Patients with Subclinical Borderline Inflammation
title_full_unstemmed	Decrease in CD14++CD16+ Monocytes in Low-Immunological-Risk Kidney Transplant Patients with Subclinical Borderline Inflammation
title_sort	decrease in cd14++cd16+ monocytes in low-immunological-risk kidney transplant patients with subclinical borderline inflammation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/d0484b89ac4a422192176ba25494a54c
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Decrease in CD14++CD16+ Monocytes in Low-Immunological-Risk Kidney Transplant Patients with Subclinical Borderline Inflammation

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