Simultaneous Quantification of 11 Phenolic Compounds and Consistency Evaluation in Four Dendrobium Species Used as Ingredients of the Traditional Chinese Medicine Shihu
The interchangeable use of different herbs to prepare the same formulation is a common practice in Traditional Chinese Medicine (TCM). However, this practice would require the component herbs to share similar compositions, at least in terms of the bioactive agents, to ensure they can replace each ot...
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Frontiers Media S.A.
2021
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oai:doaj.org-article:d04eb8ed6ce34058afe504a8f32ee4c82021-11-04T08:10:53ZSimultaneous Quantification of 11 Phenolic Compounds and Consistency Evaluation in Four Dendrobium Species Used as Ingredients of the Traditional Chinese Medicine Shihu2296-861X10.3389/fnut.2021.771078https://doaj.org/article/d04eb8ed6ce34058afe504a8f32ee4c82021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fnut.2021.771078/fullhttps://doaj.org/toc/2296-861XThe interchangeable use of different herbs to prepare the same formulation is a common practice in Traditional Chinese Medicine (TCM). However, this practice would require the component herbs to share similar compositions, at least in terms of the bioactive agents, to ensure they can replace each other in drug preparation. In this study, we developed an effective and comprehensive high-performance liquid chromatography-diode array detector (HPLC-DAD) method for simultaneous analysis of 11 phenolic compounds in the methanol extracts of Dendrobium huoshanense, Dendrobium nobile (D. nobile), Dendrobium chrysotoxum (D. chrysotoxum), and Dendrobium fimbriatum (D. fimbriatum), which have been identified as interchangeable ingredients for the same TCM preparation “Shihu” in the Chinese pharmacopeia (ChP). The consistency of the four Dendrobium species was evaluated on the basis of the presence of the 11 investigated compounds and the HPLC fingerprints of the methanol extracts of the plants. When gradient elution was performed with a solvent system of acetonitrile and water on a Zorbax Eclipse XDB-C18 (150 mm × 4.6 mm, 5 μm) with monitoring at 220 nm, all 11 investigated compounds were isolated at the baseline. The established HPLC method showed excellent linearity (all analytical curves showed relative coefficients [R2] > 0.999), sensitivity, precision (relative standard deviation [RSD] < 2%), and accuracy (recovery, 90.65–99.17%). These findings confirmed that the method we constructed was reliable. Quantification analysis showed significant differences in the contents of the investigated polyphenols in the four Dendrobium species. Evaluations of consistency revealed that the similarities among the four species were 0.299–0.906 in assessments based on the 11 polyphenols and 0.685–0.968 in assessments based on HPLC fingerprints. Thus, the components of the four Dendrobium species may be significantly different, and more experiments are required to determine whether they can be used interchangeably in the same amounts for preparing the formulation according to ChP.An-Ling ZhuAn-Ling ZhuAn-Ling ZhuJing-Wen HaoJing-Wen HaoLei LiuLei LiuQi WangQi WangNai-Dong ChenNai-Dong ChenNai-Dong ChenGuang-Lin WangGuang-Lin WangXiao-Quan LiuXiao-Quan LiuQiang LiQiang LiHui-Min XuHui-Min XuWei-Han YangWei-Han YangFrontiers Media S.A.articleDendrobiumShihuone medicine from multiple originspolyphenolconsistency evaluationNutrition. Foods and food supplyTX341-641ENFrontiers in Nutrition, Vol 8 (2021) |
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DOAJ |
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Dendrobium Shihu one medicine from multiple origins polyphenol consistency evaluation Nutrition. Foods and food supply TX341-641 |
| spellingShingle |
Dendrobium Shihu one medicine from multiple origins polyphenol consistency evaluation Nutrition. Foods and food supply TX341-641 An-Ling Zhu An-Ling Zhu An-Ling Zhu Jing-Wen Hao Jing-Wen Hao Lei Liu Lei Liu Qi Wang Qi Wang Nai-Dong Chen Nai-Dong Chen Nai-Dong Chen Guang-Lin Wang Guang-Lin Wang Xiao-Quan Liu Xiao-Quan Liu Qiang Li Qiang Li Hui-Min Xu Hui-Min Xu Wei-Han Yang Wei-Han Yang Simultaneous Quantification of 11 Phenolic Compounds and Consistency Evaluation in Four Dendrobium Species Used as Ingredients of the Traditional Chinese Medicine Shihu |
| description |
The interchangeable use of different herbs to prepare the same formulation is a common practice in Traditional Chinese Medicine (TCM). However, this practice would require the component herbs to share similar compositions, at least in terms of the bioactive agents, to ensure they can replace each other in drug preparation. In this study, we developed an effective and comprehensive high-performance liquid chromatography-diode array detector (HPLC-DAD) method for simultaneous analysis of 11 phenolic compounds in the methanol extracts of Dendrobium huoshanense, Dendrobium nobile (D. nobile), Dendrobium chrysotoxum (D. chrysotoxum), and Dendrobium fimbriatum (D. fimbriatum), which have been identified as interchangeable ingredients for the same TCM preparation “Shihu” in the Chinese pharmacopeia (ChP). The consistency of the four Dendrobium species was evaluated on the basis of the presence of the 11 investigated compounds and the HPLC fingerprints of the methanol extracts of the plants. When gradient elution was performed with a solvent system of acetonitrile and water on a Zorbax Eclipse XDB-C18 (150 mm × 4.6 mm, 5 μm) with monitoring at 220 nm, all 11 investigated compounds were isolated at the baseline. The established HPLC method showed excellent linearity (all analytical curves showed relative coefficients [R2] > 0.999), sensitivity, precision (relative standard deviation [RSD] < 2%), and accuracy (recovery, 90.65–99.17%). These findings confirmed that the method we constructed was reliable. Quantification analysis showed significant differences in the contents of the investigated polyphenols in the four Dendrobium species. Evaluations of consistency revealed that the similarities among the four species were 0.299–0.906 in assessments based on the 11 polyphenols and 0.685–0.968 in assessments based on HPLC fingerprints. Thus, the components of the four Dendrobium species may be significantly different, and more experiments are required to determine whether they can be used interchangeably in the same amounts for preparing the formulation according to ChP. |
| format |
article |
| author |
An-Ling Zhu An-Ling Zhu An-Ling Zhu Jing-Wen Hao Jing-Wen Hao Lei Liu Lei Liu Qi Wang Qi Wang Nai-Dong Chen Nai-Dong Chen Nai-Dong Chen Guang-Lin Wang Guang-Lin Wang Xiao-Quan Liu Xiao-Quan Liu Qiang Li Qiang Li Hui-Min Xu Hui-Min Xu Wei-Han Yang Wei-Han Yang |
| author_facet |
An-Ling Zhu An-Ling Zhu An-Ling Zhu Jing-Wen Hao Jing-Wen Hao Lei Liu Lei Liu Qi Wang Qi Wang Nai-Dong Chen Nai-Dong Chen Nai-Dong Chen Guang-Lin Wang Guang-Lin Wang Xiao-Quan Liu Xiao-Quan Liu Qiang Li Qiang Li Hui-Min Xu Hui-Min Xu Wei-Han Yang Wei-Han Yang |
| author_sort |
An-Ling Zhu |
| title |
Simultaneous Quantification of 11 Phenolic Compounds and Consistency Evaluation in Four Dendrobium Species Used as Ingredients of the Traditional Chinese Medicine Shihu |
| title_short |
Simultaneous Quantification of 11 Phenolic Compounds and Consistency Evaluation in Four Dendrobium Species Used as Ingredients of the Traditional Chinese Medicine Shihu |
| title_full |
Simultaneous Quantification of 11 Phenolic Compounds and Consistency Evaluation in Four Dendrobium Species Used as Ingredients of the Traditional Chinese Medicine Shihu |
| title_fullStr |
Simultaneous Quantification of 11 Phenolic Compounds and Consistency Evaluation in Four Dendrobium Species Used as Ingredients of the Traditional Chinese Medicine Shihu |
| title_full_unstemmed |
Simultaneous Quantification of 11 Phenolic Compounds and Consistency Evaluation in Four Dendrobium Species Used as Ingredients of the Traditional Chinese Medicine Shihu |
| title_sort |
simultaneous quantification of 11 phenolic compounds and consistency evaluation in four dendrobium species used as ingredients of the traditional chinese medicine shihu |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/d04eb8ed6ce34058afe504a8f32ee4c8 |
| work_keys_str_mv |
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