COVID-19 wastewater based epidemiology: long-term monitoring of 10 WWTP in France reveals the importance of the sampling context
SARS-CoV-2 wastewater-based epidemiology (WBE) has been advanced as a relevant indicator of distribution of COVID-19 in communities, supporting classical testing and tracing epidemiological approaches. An extensive sampling campaign, including ten municipal wastewater treatment plants, has been cond...
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2021
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oai:doaj.org-article:d054455e8c8844c28a49879d5110f0c52021-11-06T11:26:22ZCOVID-19 wastewater based epidemiology: long-term monitoring of 10 WWTP in France reveals the importance of the sampling context0273-12231996-973210.2166/wst.2021.418https://doaj.org/article/d054455e8c8844c28a49879d5110f0c52021-10-01T00:00:00Zhttp://wst.iwaponline.com/content/84/8/1997https://doaj.org/toc/0273-1223https://doaj.org/toc/1996-9732SARS-CoV-2 wastewater-based epidemiology (WBE) has been advanced as a relevant indicator of distribution of COVID-19 in communities, supporting classical testing and tracing epidemiological approaches. An extensive sampling campaign, including ten municipal wastewater treatment plants, has been conducted in different cities of France over a 20-week period, encompassing the second peak of COVID-19 outbreak in France. A well-recognised ultrafiltration – RNA extraction – RT-qPCR protocol was used and qualified, showing 5.5 +/− 0.5% recovery yield on heat-inactivated SARS-CoV-2. Importantly the whole, solid and liquid, fraction of wastewater was used for virus concentration in this study. Campaign results showed medium- to strong- correlation between SARS-CoV-2 WBE data and COVID-19 prevalence. To go further, statistical relationships between WWTP inlet flow rate and rainfall were studied and taken into account for each WWTP in order to calculate contextualized SARS-CoV-2 loads. This metric presented improved correlation strengths with COVID-19 prevalence for WWTP particularly submitted and sensitive to rain. Such findings highlighted that SARS-CoV-2 WBE data ultimately require to be contextualized for relevant interpretation. HIGHLIGHTS First study monitoring inlet of 10 WWTPs located in France for SARS-CoV-2 RNA quantification over a 20-week period encompassing the second peak of COVID-19 outbreak.; Viral recovery yield was 5.5% +/− 0.5% using heat-inactivated SARS-CoV-2.; Medium to high Spearman's correlation strength was observed between SARS-CoV-2 WBE and COVID-19 prevalence data.; Considering sampling context (i.e. rain events) improved data consistency and correlation strength.;A. LazukaC. ArnalE. SoyeuxM. SampsonA.-S. LepeupleY. DeleuzeS. Pouradier DuteilS. LacroixIWA Publishingarticlecovid-19public healthrt-qpcrsampling contextsars-cov-2wastewater-based epidemiologyEnvironmental technology. Sanitary engineeringTD1-1066ENWater Science and Technology, Vol 84, Iss 8, Pp 1997-2013 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
covid-19 public health rt-qpcr sampling context sars-cov-2 wastewater-based epidemiology Environmental technology. Sanitary engineering TD1-1066 |
| spellingShingle |
covid-19 public health rt-qpcr sampling context sars-cov-2 wastewater-based epidemiology Environmental technology. Sanitary engineering TD1-1066 A. Lazuka C. Arnal E. Soyeux M. Sampson A.-S. Lepeuple Y. Deleuze S. Pouradier Duteil S. Lacroix COVID-19 wastewater based epidemiology: long-term monitoring of 10 WWTP in France reveals the importance of the sampling context |
| description |
SARS-CoV-2 wastewater-based epidemiology (WBE) has been advanced as a relevant indicator of distribution of COVID-19 in communities, supporting classical testing and tracing epidemiological approaches. An extensive sampling campaign, including ten municipal wastewater treatment plants, has been conducted in different cities of France over a 20-week period, encompassing the second peak of COVID-19 outbreak in France. A well-recognised ultrafiltration – RNA extraction – RT-qPCR protocol was used and qualified, showing 5.5 +/− 0.5% recovery yield on heat-inactivated SARS-CoV-2. Importantly the whole, solid and liquid, fraction of wastewater was used for virus concentration in this study. Campaign results showed medium- to strong- correlation between SARS-CoV-2 WBE data and COVID-19 prevalence. To go further, statistical relationships between WWTP inlet flow rate and rainfall were studied and taken into account for each WWTP in order to calculate contextualized SARS-CoV-2 loads. This metric presented improved correlation strengths with COVID-19 prevalence for WWTP particularly submitted and sensitive to rain. Such findings highlighted that SARS-CoV-2 WBE data ultimately require to be contextualized for relevant interpretation. HIGHLIGHTS
First study monitoring inlet of 10 WWTPs located in France for SARS-CoV-2 RNA quantification over a 20-week period encompassing the second peak of COVID-19 outbreak.;
Viral recovery yield was 5.5% +/− 0.5% using heat-inactivated SARS-CoV-2.;
Medium to high Spearman's correlation strength was observed between SARS-CoV-2 WBE and COVID-19 prevalence data.;
Considering sampling context (i.e. rain events) improved data consistency and correlation strength.; |
| format |
article |
| author |
A. Lazuka C. Arnal E. Soyeux M. Sampson A.-S. Lepeuple Y. Deleuze S. Pouradier Duteil S. Lacroix |
| author_facet |
A. Lazuka C. Arnal E. Soyeux M. Sampson A.-S. Lepeuple Y. Deleuze S. Pouradier Duteil S. Lacroix |
| author_sort |
A. Lazuka |
| title |
COVID-19 wastewater based epidemiology: long-term monitoring of 10 WWTP in France reveals the importance of the sampling context |
| title_short |
COVID-19 wastewater based epidemiology: long-term monitoring of 10 WWTP in France reveals the importance of the sampling context |
| title_full |
COVID-19 wastewater based epidemiology: long-term monitoring of 10 WWTP in France reveals the importance of the sampling context |
| title_fullStr |
COVID-19 wastewater based epidemiology: long-term monitoring of 10 WWTP in France reveals the importance of the sampling context |
| title_full_unstemmed |
COVID-19 wastewater based epidemiology: long-term monitoring of 10 WWTP in France reveals the importance of the sampling context |
| title_sort |
covid-19 wastewater based epidemiology: long-term monitoring of 10 wwtp in france reveals the importance of the sampling context |
| publisher |
IWA Publishing |
| publishDate |
2021 |
| url |
https://doaj.org/article/d054455e8c8844c28a49879d5110f0c5 |
| work_keys_str_mv |
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