Amplification mode differs along the length of the mouse cochlea as revealed by connexin 26 deletion from specific gap junctions

Abstract The sharp frequency tuning and exquisite sensitivity of the mammalian cochlea is due to active forces delivered by outer hair cells (OHCs) to the cochlear partition. Force transmission is mediated and modulated by specialized cells, including Deiters’ cells (DCs) and pillar cells (PCs), cou...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Victoria A. Lukashkina, Tetsuji Yamashita, Jian Zuo, Andrei N. Lukashkin, Ian J. Russell
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2017
Materias:
R
Q
Acceso en línea:https://doaj.org/article/d05a16c138454ed4b1706eae3d823603
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:d05a16c138454ed4b1706eae3d823603
record_format dspace
spelling oai:doaj.org-article:d05a16c138454ed4b1706eae3d8236032021-12-02T12:32:29ZAmplification mode differs along the length of the mouse cochlea as revealed by connexin 26 deletion from specific gap junctions10.1038/s41598-017-04279-32045-2322https://doaj.org/article/d05a16c138454ed4b1706eae3d8236032017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-04279-3https://doaj.org/toc/2045-2322Abstract The sharp frequency tuning and exquisite sensitivity of the mammalian cochlea is due to active forces delivered by outer hair cells (OHCs) to the cochlear partition. Force transmission is mediated and modulated by specialized cells, including Deiters’ cells (DCs) and pillar cells (PCs), coupled by gap-junctions composed of connexin 26 (Cx26) and Cx30. We created a mouse with conditional Cx26 knock-out (Cx26 cKO) in DCs and PCs that did not influence sensory transduction, receptor-current-driving-voltage, low-mid-frequency distortion-product-otoacoustic-emissions (DPOAEs), and passive basilar membrane (BM) responses. However, the Cx26 cKO desensitizes mid-high-frequency DPOAEs and active BM responses and sensitizes low-mid-frequency neural excitation. This functional segregation may indicate that the flexible, apical turn cochlear partition facilitates transfer of OHC displacements (isotonic forces) for cochlear amplification and neural excitation. DC and PC Cx26 expression is essential for cochlear amplification in the stiff basal turn, possibly through maintaining cochlear partition mechanical impedance, thereby ensuring effective transfer of OHC isometric forces.Victoria A. LukashkinaTetsuji YamashitaJian ZuoAndrei N. LukashkinIan J. RussellNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Victoria A. Lukashkina
Tetsuji Yamashita
Jian Zuo
Andrei N. Lukashkin
Ian J. Russell
Amplification mode differs along the length of the mouse cochlea as revealed by connexin 26 deletion from specific gap junctions
description Abstract The sharp frequency tuning and exquisite sensitivity of the mammalian cochlea is due to active forces delivered by outer hair cells (OHCs) to the cochlear partition. Force transmission is mediated and modulated by specialized cells, including Deiters’ cells (DCs) and pillar cells (PCs), coupled by gap-junctions composed of connexin 26 (Cx26) and Cx30. We created a mouse with conditional Cx26 knock-out (Cx26 cKO) in DCs and PCs that did not influence sensory transduction, receptor-current-driving-voltage, low-mid-frequency distortion-product-otoacoustic-emissions (DPOAEs), and passive basilar membrane (BM) responses. However, the Cx26 cKO desensitizes mid-high-frequency DPOAEs and active BM responses and sensitizes low-mid-frequency neural excitation. This functional segregation may indicate that the flexible, apical turn cochlear partition facilitates transfer of OHC displacements (isotonic forces) for cochlear amplification and neural excitation. DC and PC Cx26 expression is essential for cochlear amplification in the stiff basal turn, possibly through maintaining cochlear partition mechanical impedance, thereby ensuring effective transfer of OHC isometric forces.
format article
author Victoria A. Lukashkina
Tetsuji Yamashita
Jian Zuo
Andrei N. Lukashkin
Ian J. Russell
author_facet Victoria A. Lukashkina
Tetsuji Yamashita
Jian Zuo
Andrei N. Lukashkin
Ian J. Russell
author_sort Victoria A. Lukashkina
title Amplification mode differs along the length of the mouse cochlea as revealed by connexin 26 deletion from specific gap junctions
title_short Amplification mode differs along the length of the mouse cochlea as revealed by connexin 26 deletion from specific gap junctions
title_full Amplification mode differs along the length of the mouse cochlea as revealed by connexin 26 deletion from specific gap junctions
title_fullStr Amplification mode differs along the length of the mouse cochlea as revealed by connexin 26 deletion from specific gap junctions
title_full_unstemmed Amplification mode differs along the length of the mouse cochlea as revealed by connexin 26 deletion from specific gap junctions
title_sort amplification mode differs along the length of the mouse cochlea as revealed by connexin 26 deletion from specific gap junctions
publisher Nature Portfolio
publishDate 2017
url https://doaj.org/article/d05a16c138454ed4b1706eae3d823603
work_keys_str_mv AT victoriaalukashkina amplificationmodediffersalongthelengthofthemousecochleaasrevealedbyconnexin26deletionfromspecificgapjunctions
AT tetsujiyamashita amplificationmodediffersalongthelengthofthemousecochleaasrevealedbyconnexin26deletionfromspecificgapjunctions
AT jianzuo amplificationmodediffersalongthelengthofthemousecochleaasrevealedbyconnexin26deletionfromspecificgapjunctions
AT andreinlukashkin amplificationmodediffersalongthelengthofthemousecochleaasrevealedbyconnexin26deletionfromspecificgapjunctions
AT ianjrussell amplificationmodediffersalongthelengthofthemousecochleaasrevealedbyconnexin26deletionfromspecificgapjunctions
_version_ 1718394063346991104