Molecular parentage analysis is essential in breeding Asian seabass.

In aquaculture species, maintaining pedigree information and genetic variation in each generation is essential, but very difficult. In this study, we used nine microsatellites to genotype 2,520 offspring from four independent full-factorial crosses (10 males × 10 females) of Asian seabass to reconst...

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Autores principales:	Peng Liu, Jun Hong Xia, Grace Lin, Fei Sun, Feng Liu, Huan Sein Lim, Hong Yan Pang, Gen Hua Yue
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/d05c430921e14d4ea3b89122490a0a69
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spelling	oai:doaj.org-article:d05c430921e14d4ea3b89122490a0a692021-11-18T08:06:13ZMolecular parentage analysis is essential in breeding Asian seabass.1932-620310.1371/journal.pone.0051142https://doaj.org/article/d05c430921e14d4ea3b89122490a0a692012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23227245/?tool=EBIhttps://doaj.org/toc/1932-6203In aquaculture species, maintaining pedigree information and genetic variation in each generation is essential, but very difficult. In this study, we used nine microsatellites to genotype 2,520 offspring from four independent full-factorial crosses (10 males × 10 females) of Asian seabass to reconstruct pedigree and monitor the change of genetic variations. In all four crosses, over 96.8% of the offspring could be assigned to their parents, indicating the high power of the nine microsatellites for parentage assignment. This study revealed several interesting results: (1). In all four crosses, the contribution of parents to offspring was significantly uneven, and some dominant breeding fishes (i.e. brooders) were found; (2). In two mass crosses where the brooders were carefully checked for reproductive status, a majority (≥ 90%) of brooders contributed to offspring, whereas in another two crosses, where the brooders were randomly picked without checking reproductive status, only a few brooders (40.0-45.0%) produced offspring; (3). Females had more problems in successful spawning compared to males; and (4). In the two crosses where a few brooders produced offspring, there was a substantial loss in allelic (24.1-34.3%) and gene (20.5-25.7%) diversities in offspring, while in the other two crosses, the majority of allelic (96.8-97.0%) and gene diversities (94.8-97.1%) were maintained. These observations suggest that a routine molecular parentage analysis is required to maintain both allelic and gene diversity in breeding Asian seabass.Peng LiuJun Hong XiaGrace LinFei SunFeng LiuHuan Sein LimHong Yan PangGen Hua YuePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e51142 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Peng Liu Jun Hong Xia Grace Lin Fei Sun Feng Liu Huan Sein Lim Hong Yan Pang Gen Hua Yue Molecular parentage analysis is essential in breeding Asian seabass.
description	In aquaculture species, maintaining pedigree information and genetic variation in each generation is essential, but very difficult. In this study, we used nine microsatellites to genotype 2,520 offspring from four independent full-factorial crosses (10 males × 10 females) of Asian seabass to reconstruct pedigree and monitor the change of genetic variations. In all four crosses, over 96.8% of the offspring could be assigned to their parents, indicating the high power of the nine microsatellites for parentage assignment. This study revealed several interesting results: (1). In all four crosses, the contribution of parents to offspring was significantly uneven, and some dominant breeding fishes (i.e. brooders) were found; (2). In two mass crosses where the brooders were carefully checked for reproductive status, a majority (≥ 90%) of brooders contributed to offspring, whereas in another two crosses, where the brooders were randomly picked without checking reproductive status, only a few brooders (40.0-45.0%) produced offspring; (3). Females had more problems in successful spawning compared to males; and (4). In the two crosses where a few brooders produced offspring, there was a substantial loss in allelic (24.1-34.3%) and gene (20.5-25.7%) diversities in offspring, while in the other two crosses, the majority of allelic (96.8-97.0%) and gene diversities (94.8-97.1%) were maintained. These observations suggest that a routine molecular parentage analysis is required to maintain both allelic and gene diversity in breeding Asian seabass.
format	article
author	Peng Liu Jun Hong Xia Grace Lin Fei Sun Feng Liu Huan Sein Lim Hong Yan Pang Gen Hua Yue
author_facet	Peng Liu Jun Hong Xia Grace Lin Fei Sun Feng Liu Huan Sein Lim Hong Yan Pang Gen Hua Yue
author_sort	Peng Liu
title	Molecular parentage analysis is essential in breeding Asian seabass.
title_short	Molecular parentage analysis is essential in breeding Asian seabass.
title_full	Molecular parentage analysis is essential in breeding Asian seabass.
title_fullStr	Molecular parentage analysis is essential in breeding Asian seabass.
title_full_unstemmed	Molecular parentage analysis is essential in breeding Asian seabass.
title_sort	molecular parentage analysis is essential in breeding asian seabass.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/d05c430921e14d4ea3b89122490a0a69
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Molecular parentage analysis is essential in breeding Asian seabass.

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