Comodulation of NO-Dependent Vasodilation by Erythroid Band 3 and Hemoglobin: A GP.Mur Athlete Study

GP.Mur, a red blood cell (RBC) hybrid protein encoded by glycophorin B-A-B, increases expression of erythroid band 3 (Anion Exchanger-1, SLC4A1). GP.Mur is extremely rare but has a prevalence of 1–10% in regions of Southeast Asia. We unexpectedly found slightly higher blood pressure (BP) among healt...

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Autores principales:	Kate Hsu, Yen-Yu Liu, Wei-Chin Tseng, Kuang-Tse Huang, Chia-Yuan Liu, Li-Yang Chen, Hui-Lin Lee, Hui-Ju Lin, Kuo-Wei Tseng, Hung-I Yeh
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	flow-mediated vasodilation (FMD) nitric oxide (NO) Band 3 GP.Mur red blood cell (RBC) hemoglobin (Hb) Diseases of the circulatory (Cardiovascular) system RC666-701
Acceso en línea:	https://doaj.org/article/d05cd3cdf4744cd783afc52f3eb916e3
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spelling	oai:doaj.org-article:d05cd3cdf4744cd783afc52f3eb916e32021-12-01T13:12:46ZComodulation of NO-Dependent Vasodilation by Erythroid Band 3 and Hemoglobin: A GP.Mur Athlete Study2297-055X10.3389/fcvm.2021.740100https://doaj.org/article/d05cd3cdf4744cd783afc52f3eb916e32021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fcvm.2021.740100/fullhttps://doaj.org/toc/2297-055XGP.Mur, a red blood cell (RBC) hybrid protein encoded by glycophorin B-A-B, increases expression of erythroid band 3 (Anion Exchanger-1, SLC4A1). GP.Mur is extremely rare but has a prevalence of 1–10% in regions of Southeast Asia. We unexpectedly found slightly higher blood pressure (BP) among healthy Taiwanese adults with GP.Mur. Since band 3 has been suggested to interact with hemoglobin (Hb) to modulate nitric oxide (NO)-dependent hypoxic vasodilation during the respiratory cycle, we hypothesized that GP.Mur red cells could exert differentiable effects on vascular tone. Here we recruited GP.Mur-positive and GP.Mur-negative elite male college athletes, as well as age-matched, GP.Mur-negative non-athletes, for NO-dependent flow-mediated dilation (FMD) and NO-independent dilation (NID). The subjects were also tested for plasma nitrite and nitrate before and after arterial occlusion in FMD. GP.Mur+ and non-GP.Mur athletes exhibited similar heart rates and blood pressure, but GP.Mur+ athletes showed significantly lower FMD (4.8 ± 2.4%) than non-GP.Mur athletes (6.5 ± 2.1%). NO-independent vasodilation was not affected by GP.Mur. As Hb controls intravascular NO bioavailability, we examined the effect of Hb on limiting FMD and found it to be significantly stronger in GP.Mur+ subjects. Biochemically, plasma nitrite levels were directly proportional to individual band 3 expression on the red cell membrane. The increase of plasma nitrite triggered by arterial occlusion also showed small dependency on band 3 levels in non-GP.Mur subjects. By the GP.Mur comparative study, we unveiled comodulation of NO-dependent vasodilation by band 3 and Hb, and verified the long-pending role of erythroid band 3 in this process.Kate HsuKate HsuKate HsuYen-Yu LiuYen-Yu LiuWei-Chin TsengKuang-Tse HuangChia-Yuan LiuChia-Yuan LiuLi-Yang ChenHui-Lin LeeHui-Ju LinKuo-Wei TsengHung-I YehHung-I YehFrontiers Media S.A.articleflow-mediated vasodilation (FMD)nitric oxide (NO)Band 3GP.Murred blood cell (RBC)hemoglobin (Hb)Diseases of the circulatory (Cardiovascular) systemRC666-701ENFrontiers in Cardiovascular Medicine, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	flow-mediated vasodilation (FMD) nitric oxide (NO) Band 3 GP.Mur red blood cell (RBC) hemoglobin (Hb) Diseases of the circulatory (Cardiovascular) system RC666-701
spellingShingle	flow-mediated vasodilation (FMD) nitric oxide (NO) Band 3 GP.Mur red blood cell (RBC) hemoglobin (Hb) Diseases of the circulatory (Cardiovascular) system RC666-701 Kate Hsu Kate Hsu Kate Hsu Yen-Yu Liu Yen-Yu Liu Wei-Chin Tseng Kuang-Tse Huang Chia-Yuan Liu Chia-Yuan Liu Li-Yang Chen Hui-Lin Lee Hui-Ju Lin Kuo-Wei Tseng Hung-I Yeh Hung-I Yeh Comodulation of NO-Dependent Vasodilation by Erythroid Band 3 and Hemoglobin: A GP.Mur Athlete Study
description	GP.Mur, a red blood cell (RBC) hybrid protein encoded by glycophorin B-A-B, increases expression of erythroid band 3 (Anion Exchanger-1, SLC4A1). GP.Mur is extremely rare but has a prevalence of 1–10% in regions of Southeast Asia. We unexpectedly found slightly higher blood pressure (BP) among healthy Taiwanese adults with GP.Mur. Since band 3 has been suggested to interact with hemoglobin (Hb) to modulate nitric oxide (NO)-dependent hypoxic vasodilation during the respiratory cycle, we hypothesized that GP.Mur red cells could exert differentiable effects on vascular tone. Here we recruited GP.Mur-positive and GP.Mur-negative elite male college athletes, as well as age-matched, GP.Mur-negative non-athletes, for NO-dependent flow-mediated dilation (FMD) and NO-independent dilation (NID). The subjects were also tested for plasma nitrite and nitrate before and after arterial occlusion in FMD. GP.Mur+ and non-GP.Mur athletes exhibited similar heart rates and blood pressure, but GP.Mur+ athletes showed significantly lower FMD (4.8 ± 2.4%) than non-GP.Mur athletes (6.5 ± 2.1%). NO-independent vasodilation was not affected by GP.Mur. As Hb controls intravascular NO bioavailability, we examined the effect of Hb on limiting FMD and found it to be significantly stronger in GP.Mur+ subjects. Biochemically, plasma nitrite levels were directly proportional to individual band 3 expression on the red cell membrane. The increase of plasma nitrite triggered by arterial occlusion also showed small dependency on band 3 levels in non-GP.Mur subjects. By the GP.Mur comparative study, we unveiled comodulation of NO-dependent vasodilation by band 3 and Hb, and verified the long-pending role of erythroid band 3 in this process.
format	article
author	Kate Hsu Kate Hsu Kate Hsu Yen-Yu Liu Yen-Yu Liu Wei-Chin Tseng Kuang-Tse Huang Chia-Yuan Liu Chia-Yuan Liu Li-Yang Chen Hui-Lin Lee Hui-Ju Lin Kuo-Wei Tseng Hung-I Yeh Hung-I Yeh
author_facet	Kate Hsu Kate Hsu Kate Hsu Yen-Yu Liu Yen-Yu Liu Wei-Chin Tseng Kuang-Tse Huang Chia-Yuan Liu Chia-Yuan Liu Li-Yang Chen Hui-Lin Lee Hui-Ju Lin Kuo-Wei Tseng Hung-I Yeh Hung-I Yeh
author_sort	Kate Hsu
title	Comodulation of NO-Dependent Vasodilation by Erythroid Band 3 and Hemoglobin: A GP.Mur Athlete Study
title_short	Comodulation of NO-Dependent Vasodilation by Erythroid Band 3 and Hemoglobin: A GP.Mur Athlete Study
title_full	Comodulation of NO-Dependent Vasodilation by Erythroid Band 3 and Hemoglobin: A GP.Mur Athlete Study
title_fullStr	Comodulation of NO-Dependent Vasodilation by Erythroid Band 3 and Hemoglobin: A GP.Mur Athlete Study
title_full_unstemmed	Comodulation of NO-Dependent Vasodilation by Erythroid Band 3 and Hemoglobin: A GP.Mur Athlete Study
title_sort	comodulation of no-dependent vasodilation by erythroid band 3 and hemoglobin: a gp.mur athlete study
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/d05cd3cdf4744cd783afc52f3eb916e3
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Comodulation of NO-Dependent Vasodilation by Erythroid Band 3 and Hemoglobin: A GP.Mur Athlete Study

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